Chili pepper extends lifespan in a concentration-dependent manner and confers cold resistance on <i>Drosophila melanogaster</i> cohorts by influencing specific metabolic pathways

Semaniuk, Uliana; Gospodaryov, Dmytro V.; Strilbytska, Olha; Kucharska, Alicja Z.; Sokół‐Łętowska, Anna; Burdyliuk, Nadia; Storey, Kenneth B.; Bayliak, Maria M.; Lushchak, Oleh

doi:10.1039/d2fo00930g

Cited by 6 publications

(11 citation statements)

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“…Previously, we have shown that consumption of chili-supplemented food extended mean lifespan in D. melanogaster cohorts of both sexes by 10%-14%, although effect was more pronounced in females [ 3 ]. Significant lifespan extension in males was caused by food supplemented with 0.04% and 0.12% chili powder.…”

Section: Resultsmentioning

confidence: 99%

“…Capsaicin, a constituent of chili pepper, was found to prolong lifespan in the fruit fly Drosophila melanogaster [ 2 ]. Our recent study has revealed that fruit flies reared on the food supplemented with chili powder live longer than the counterparts on the control diet [ 3 ]. The effect can be accounted for by capsaicin as well as by other phenolic substances in chili pepper.…”

Section: Discussionmentioning

confidence: 99%

“…10 min) carbon dioxide anaesthesia and put into mortality cages at a density of approx. 150 individuals per cage [ 3 ]. All females used in experiments were mated.…”

Section: Methodsmentioning

confidence: 99%

“…It was recently shown that capsaicin, a derivative of the phenol-containing compound, vanillylamine and a pungent principle component of chili pepper, prolongs lifespan in the fruit fly, Drosophila melanogaster , model [ 2 ]. We have conducted a complex study [ 3 ] to find whether the same capability to extend lifespan is attributable to chili powder, and not only to pure capsaicin. As in the above study of capsaicin effects [ 2 ], we used D. melanogaster as a tractable model, very suitable for a quick and inexpensive study.…”

Section: Introductionmentioning

confidence: 99%

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Chili-supplemented food decreases glutathione-S-transferase activity in Drosophila melanogaster females without a change in other parameters of antioxidant system

et al. 2022

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Objectives Many plant-derived anti-aging preparations influence antioxidant defense system. Consumption of food supplemented with chili pepper powder was found to extend lifespan in the fruit fly, Drosophila melanogaster . The present study aimed to test a connection between life-extending effect of chili powder and antioxidant defense system of D. melanogaster . Methods Flies were reared for 15 days in the mortality cages on food with 0% (control), 0.04%, 0.12%, 0.4%, or 3% chili powder. Antioxidant and related enzymes, as well as oxidative stress indices were measured. Results Female flies that consumed chili-supplemented food had a 40–60% lower glutathione- S -transferase (GST) activity as compared with the control cohort. Activity of superoxide dismutase (SOD) was about 37% higher in males that consumed food with 3% chili powder in comparison with the control cohort. Many of the parameters studied were sex-dependent. Conclusions Consumption of chili-supplemented food extends lifespan in fruit fly cohorts in a concentration- and gender-dependent manner. However, this extension is not mediated by a strengthening of antioxidant defenses. Consumption of chili-supplemented food does not change the specific relationship between antioxidant and related enzymes in D. melanogaster , and does not change the linkage of the activities of these enzymes to fly gender.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…10 min) carbon dioxide anaesthesia and put into mortality cages at a density of approx. 150 individuals per cage [ 3 ]. All females used in experiments were mated.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chili-supplemented food decreases glutathione-S-transferase activity in Drosophila melanogaster females without a change in other parameters of antioxidant system

et al. 2022

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“…Dietary capsaicin consumption could have a beneficial effect on weight management by activating brown adipose tissue activity and reducing energy intake via appetite and satiety regulation [ 26 ]. In Drosophila, capsaicin effects have been described displaying contrasting responses, for instance, Li et al, [ 27 ] showed that capsaicin repels Drosophila females from oviposition, meanwhile, Semaniuk et al [ 28 ] reported that chili pepper extends lifespan in a concentration-dependent manner and also confers cold resistance. Our food intake analysis revealed that there was a slight reduction in the food consumption of flies reared on the habanero-pepper diet when compared to control; however, this reduction was not significant indicating that the habanero-pepper diet did not induce a repulsive response in Drosophila flies.…”

Section: Discussionmentioning

confidence: 99%

Peppers in Diet: Genome-Wide Transcriptome and Metabolome Changes in Drosophila melanogaster

Lopez-Ortiz

Edwards

Natarajan

et al. 2022

IJMS

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The habanero pepper (Capsicum chinense) is an increasingly important spice and vegetable crop worldwide because of its high capsaicin content and pungent flavor. Diets supplemented with the phytochemicals found in habanero peppers might cause shifts in an organism’s metabolism and gene expression. Thus, understanding how these interactions occur can reveal the potential health effects associated with such changes. We performed transcriptomic and metabolomic analyses of Drosophila melanogaster adult flies reared on a habanero pepper diet. We found 539 genes/59 metabolites that were differentially expressed/accumulated in flies fed a pepper versus control diet. Transcriptome results indicated that olfactory sensitivity and behavioral responses to the pepper diet were mediated by olfactory and nutrient-related genes including gustatory receptors (Gr63a, Gr66a, and Gr89a), odorant receptors (Or23a, Or59a, Or82a, and Orco), and odorant-binding proteins (Obp28a, Obp83a, Obp83b, Obp93a, and Obp99a). Metabolome analysis revealed that campesterol, sitosterol, and sucrose were highly upregulated and azelaic acid, ethyl phosphoric acid, and citric acid were the major metabolites downregulated in response to the habanero pepper diet. Further investigation by integration analysis between transcriptome and metabolome data at gene pathway levels revealed six unique enriched pathways, including phenylalanine metabolism; insect hormone biosynthesis; pyrimidine metabolism; glyoxylate, and dicarboxylate metabolism; glycine, serine, threonine metabolism; and glycerolipid metabolism. In view of the transcriptome and metabolome findings, our comprehensive analysis of the response to a pepper diet in Drosophila have implications for exploring the molecular mechanism of pepper consumption.

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Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Demianchuk,

Lylyk,

Balatskiy

et al. 2024

Arch Insect Biochem Physiol

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Studies on antiaging remedies in insect models sometimes show discrepancies in results. These discrepancies could be explained by different responses of short‐ and long‐lived strains on the antiaging remedies. The purpose of the study was to test whether life‐prolonging effects of alpha‐ketoglutarate (AKG), observed in nematodes and fruit flies, would be reproduced in long‐lived Drosophila melanogaster flies. Lifespan was assayed in flies kept in demographic cages. Fecundity, proportion of flies capable of negative geotaxis, starvation resistance, time of heat coma onset, levels of triacyglycerols, body glucose, glycogen, activities of glutamate dehydrogenase, catalase, glutathione‐S‐transferase, hexokinase, phosphofructokinase, pyruvate kinase, lactate, and glutamate dehydrogenases were assessed. Dietary AKG did not affect fly lifespan on the diet with 5% yeast and 5% sucrose (5Y:5S) and on the diet with 9% yeast and 1% sucrose (9Y:1S), but increased lifespan on the low‐protein diet (1Y:9S). Twenty‐five‐day‐old female flies fed a 5Y:5S diet with 10 mM AKG for 3 weeks, did not differ from the control group (without AKG) in climbing activity, resistance to heat stress, and starvation. The levels of glucose and glycogen were unaffected but the levels of triacylglycerols were lower in AKG‐fed female flies. No differences in activities of glycolytic enzymes, NADPH‐producing enzymes, glutamate dehydrogenase, oxygen consumption, and levels of oxidative stress markers were observed between the control and AKG‐fed flies. However, AKG‐fed flies had lower activities of catalase and glutathione‐S‐transferase. These results suggest that potential antiaging remedies, such as AKG, may not extend lifespan in long‐living organisms despite influencing several metabolic parameters.

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Chili pepper extends lifespan in a concentration-dependent manner and confers cold resistance on Drosophila melanogaster cohorts by influencing specific metabolic pathways

Abstract: Chili powder is a widely used spice with pungent taste, often consumed on a daily basis in several countries. Recent prospective cohort studies showed that the regular use of chili...

Cited by 6 publications

References 79 publications

Chili-supplemented food decreases glutathione-S-transferase activity in Drosophila melanogaster females without a change in other parameters of antioxidant system

Chili-supplemented food decreases glutathione-S-transferase activity in Drosophila melanogaster females without a change in other parameters of antioxidant system

Peppers in Diet: Genome-Wide Transcriptome and Metabolome Changes in Drosophila melanogaster

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

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