Children have similar RT-PCR cycle threshold for SARS-CoV-2 in comparison with adults

Polese-Bonatto, Márcia; Its, Sartor; Hammes-Varela, F; Glt, Gianinni; Tr, Azevedo; Ir, Fernandes; Go, Zavaglia; Kern, Luciane Beatriz; Ap, Santos; Cn, de David; Waf, de Almeida; Vbg, Porto; Mc, Scotta; Rt, Stein

doi:10.1101/2021.04.20.21255059

Cited by 6 publications

(3 citation statements)

References 23 publications

(44 reference statements)

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“…At enrollment, oropharyngeal and bilateral nasopharyngeal swab collection for SARS-CoV-2 detection was performed for all participants and analyzed through qualitative reverse transcription polymerase chain reaction (RT-PCR) assay, as described elsewhere [24] . Participants with detection of SARS-CoV-2, regardless of the occurrence of other respiratory pathogens co-detections, were classified as having COVID-19.…”

Section: Methodsmentioning

confidence: 99%

Impact of rhinovirus on hospitalization during the COVID-19 pandemic: A prospective cohort study.

Scottá

Kern²,

Polese-Bonatto³

et al. 2022

Journal of Clinical Virology

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Section: Methodsmentioning

confidence: 99%

Impact of rhinovirus on hospitalization during the COVID-19 pandemic: A prospective cohort study.

Scottá

Kern²,

Polese-Bonatto³

et al. 2022

Journal of Clinical Virology

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“…All participants performed qualitative RT-PCR assay (TaqManTM 2019-nCoV Kit v1, catalog number A47532, ThermoFisher Scientific, Pleasanton, California, EUA) to SARS-CoV-2 detection as described elsewhere ( Polese-Bonatto et al, 2021 ). Additionally, S gene dropout samples with cycle threshold less than 30 (Ct < 30.0) were submitted to high-throughput sequencing (HTS) using the Illumina MiSeq.…”

Section: Methodsmentioning

confidence: 99%

Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction

Sartor,

Varela,

Meireles

et al. 2022

Diagnostic Microbiology and Infectious Disease

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We aimed to describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize the receptor-binding domain (RBD) of viral spike protein. Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October in Porto Alegre, Brazil. All participants performed RT-PCR assay, and samples with S gene dropout and cycle threshold < 30 were submitted to high-throughput sequencing (HTS). 484 out of 1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) and a peak was observed in August. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein. The Y380Q and C379W may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304).

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“…All participants underwent bilateral nasopharyngeal and oropharyngeal swab collection for SARS-CoV-2 detection via RT-PCR assay. The samples were analyzed in the Molecular Biology Laboratory at Hospital Moinhos de Vento, as described previously (6). Reported weight and height were used to calculate body mass index (BMI).…”

Section: Study Proceduresmentioning

confidence: 99%

Association between obesity and hospitalization in mild COVID-19 adult outpatients in Brazil: a prospective cohort study

Sartor¹,

David²,

Teló³

et al. 2022

Archives of Endocrinology and Metabolism

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Objective: To evaluate the association between obesity and hospitalization in mild COVID-19 adult outpatients in Brazil. Subjects and methods: Adults with signs and symptoms suggestive of acute SARS-CoV-2 infection who sought treatment in two hospital (public and private) emergency departments were prospectively enrolled. Patients with confirmed COVID-19 at inclusion were followed by phone calls at days D7, D14 and D28. Multivariable logistic regression models were employed to explore the association between obesity and other potential predictors for hospitalization. Results: A total of 1,050 participants were screened, and 297 completed the 28-day follow-up and were diagnosed with COVID-19 by RT-PCR. The median age was 37.2 (IQR 29.7-44.6) years, and 179 (60.0%) were female. The duration of symptoms was 3.0 (IQR 2.0-5.0) days, and 10.0 (IQR 8.0-12.0) was the median number of symptoms at inclusion. Ninety-five (32.0%) individuals had obesity, and 233 (78.5%) had no previous medical conditions. Twentythree participants (7.7%) required hospitalization during the follow-up period. After adjusting, obesity (BMI ≥ 30.0 kg/m 2 ) (OR = 2.69, 95% CI 1.63-4.83, P < 0.001) and older age (OR = 1.05, 95% CI 1.01-1.09, P < 0.001) were significantly associated with higher risks of hospitalization. Conclusion: Obesity, followed by aging, was the main factor associated with hospital admission for COVID-19 in a young population in a lowmiddle income country. Our findings highlighted the need to promote additional protection for individuals with obesity, such as vaccination, and to encourage lifestyle changes.

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Children have similar RT-PCR cycle threshold for SARS-CoV-2 in comparison with adults

Cited by 6 publications

References 23 publications

Impact of rhinovirus on hospitalization during the COVID-19 pandemic: A prospective cohort study.

Impact of rhinovirus on hospitalization during the COVID-19 pandemic: A prospective cohort study.

Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction

Association between obesity and hospitalization in mild COVID-19 adult outpatients in Brazil: a prospective cohort study

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