Chicken-to-Human Infection with Campylobacter jejuni and Campylobacter coli: Biotype and Serotype Correlation

Annan-Prah, A.; Janc, M.

doi:10.4315/0362-028x-51.7.562

Cited by 22 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Campylobacteriosis is mainly a food-borne infection, and poultry products may play an important role in the transmission to humans [1,8,10,21]. C. jejuni contamination of poultry meat during processing has been well documented [2,11,18,24].…”

Section: Methodsmentioning

confidence: 99%

Analysis of Distribution of Campylobacter jejuni and Campylobacter coli in Broilers by Using Restriction Fragment Length Polymorphism of Flagellin Gene.

Chuma

Makino

Okamoto

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The incidence of Campylobacter jejuni and Campylobacter coli in broiler farms was 33.9% (19/56). C. jejuni-positive flocks accounted for 20.0% (17/85) and C. coli-positive ones was 4.7% (4/85). There were 14 patterns (fla type) of restriction fragment length polymorphism (RFLP) of flagellin A gene among these 22 strains of C. jejuni and C. coli including the standard strain C. jejuni ATCC 33560. Different fla types of Campylobacter were isolated from broilers in different growing cycles on the same farms. Four strains of C. jejuni were isolated from four breeder farms and four fla types of C. jejuni were detected from their progenies reared on growing farms. Three fla types of C. jejuni detected from the progenies were different from those of each breeder. Also, the other three fla types of C. jejuni were detected from different progenies of each growing farm during the next growing cycle. These findings indicate that the RFLP analysis may contribute to epidemiological studies of C. jejuni and C. coli contamination of broilers and suggest the risk of contamination with different types of Campylobacter in every growing cycle of broilers on the farm even on the same farm. They also supported that there was little likeliness of the vertical transmission of C. jejuni and C. coli from breeders to broilers. -KEY WORDS : broiler, Campylobacter jejuni, flagellin gene typing, PCR, restriction fragment length polymorphism.

show abstract

Section: Methodsmentioning

confidence: 99%

Analysis of Distribution of Campylobacter jejuni and Campylobacter coli in Broilers by Using Restriction Fragment Length Polymorphism of Flagellin Gene.

Chuma

Makino

Okamoto

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…Serotypes from poultry only partly agree with isolates from human patients. According to the study by Annan-Prah (1988), such serotypes as HS 1, 2, 5, 7, 9 and 22 were most frequently isolated from slaughtered poultry and ill human patients. The most prevalent serotype was HS 2.…”

Section: Discussionmentioning

confidence: 99%

“…Poultry, hosting very frequently C. jejuni, are considered as one of the main sources of the agent. A great number of papers (Aarts et al 1995;Annan-Prah et al 1988;Jacobs-Reitsma et al 1995) were engaged in studying and comparing serotypes isolated from patients and poultry. Serotypes from poultry only partly agree with isolates from human patients.…”

Section: Discussionmentioning

confidence: 99%

Serotyping and Identification of Campylobacter jejuni and Campylobacter coli Strains of Human and Animal Origin Using the PCR Method

Steinhauserova¹,

Fojtíková²

1999

Acta Vet. Brno

View full text Add to dashboard Cite

Steinhauserová I., K. Fojtíková: Serotyping and Identification of Campylobacter jejuni and Campylobacter coli Strains of Human and Animal Origin Using the PCR Method. Acta Vet. Brno 1999, 68: 149-154. A total number of 178 strains of Campylobacter spp. from human patients suffering from diarrhea and from the intestinal content of slaughtered pigs and poultry was isolated during years 1996 to 1997. Campylobacter strains were isolated and identified by standard tests and the PCR method using fla A and fla B, as well. Isolates originating from human patients were identified as Campylobacter jejuni (98% = 108 strains) and C. coli (2% = 2 strains). Campylobacter spp. (20) strains obtained from slaughtered pigs were identified using standard tests in 45% (9 strains) and 40% (8 strains) as C. coli and C. jejuni, respectively. Strains of Campylobacter spp. (48) isolated from slaughtered poultry were identified as C. jejuni (89% = 42 strains). Standard tests failed to identify 9 strains; 6 strains were identified as Campylobacter jejuni and C. coli using the PCR method. Serotyping using 34 antisera of the Penner's serotyping scheme was performed in 130 strains of Campylobacter jejuni isolated from poultry and human patients. This method was suitable for identification of almost 80% of C. jejuni strains. We found 10 different serotypes of human C. jejuni strains. There were serotypes (HS) 4, 1 and 9 (32%, 23% and 15%, respectively) predominating in the human population; they were followed by serotypes (HS) 10, 2 and 23 (8%, 5% and 3%, respectively). Animal strains included 7 (HS) serotypes. Like in the human strains, the serotype 4, found in 37% of examined isolates, was predominating. Serotypes 23 and 1 (17% and 15%, respectively) were the next most frequent ones. Comparing the obtained serotypes of human and animal strains we see that there are only 4 identical ones (i.e., 4, 1, 10, 23), which are, however, most frequently occurring in both groups of strains.The PCR methods and serotyping may facilitate and make more accurate the results of standard cultivation and in epidemiologically serious cases help in explaining the route of transmission.

show abstract

“…are common among the intestinal flora in normal broiler chickens, and the majority of commercial flocks are infected (13). Conventional typing methods, such as biotyping and serotyping, have been used to investigate the epidemiology of Campylobacter infections (1,16). However, the limited discriminating ability of these methods restricts their application for investigating likely sources of Campylobacter spp.…”

mentioning

confidence: 99%

Genotypic Diversity among Campylobacter jejuni Isolates in a Commercial Broiler Flock

Thomas¹,

Long²,

Good³

et al. 1997

Appl Environ Microbiol

View full text Add to dashboard Cite

Analysis of nucleic acid polymorphism in the flagellin genes of Campylobacter jejuni was used to investigate genetic diversity among Campylobacter spp. in a commercial broiler flock. Three hundred single colonies of C. jejuni were isolated from fecal samples collected weekly for 3 weeks immediately before slaughter. Both the flaA and flaB genes were amplified by PCR, and the PCR product was digested with the restriction enzyme AluI. The fragments generated were then analyzed by agarose gel electrophoresis. Among the 300 recovered isolates, five different restriction fragment length polymorphism profiles were observed. Three of these profiles were dominant during the course of the study, and the other two profiles were detected at low frequency. Analysis of genetic variation in C. jejuni over the course of an experimental infection lasting 7 weeks indicated that there was no obvious drift in the flagellin gene type. These findings demonstrate that a range of bacterial genotypes can constitute the bacterial population within a commercial poultry flock, with the most likely sources of these types being multiple environmental exposure and/or genetic drift within the population. This degree of diversity must be considered in epidemiological analyses which utilize genetic typing methods that investigate Campylobacter contamination of any food source, including poultry, to ensure that the total gene pool for C. jejuni is evaluated.

show abstract

Chicken-to-Human Infection with Campylobacter jejuni and Campylobacter coli: Biotype and Serotype Correlation

Cited by 22 publications

References 0 publications

Analysis of Distribution of Campylobacter jejuni and Campylobacter coli in Broilers by Using Restriction Fragment Length Polymorphism of Flagellin Gene.

Analysis of Distribution of Campylobacter jejuni and Campylobacter coli in Broilers by Using Restriction Fragment Length Polymorphism of Flagellin Gene.

Serotyping and Identification of Campylobacter jejuni and Campylobacter coli Strains of Human and Animal Origin Using the PCR Method

Genotypic Diversity among Campylobacter jejuni Isolates in a Commercial Broiler Flock

Contact Info

Product

Resources

About