Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV

Padgett, Thomas; Stubblefield, Elton; Varmus, Harold

doi:10.1016/0092-8674(77)90098-8

Cited by 75 publications

(42 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromosomes were fractionated by sucrose gradient centrifugation (10,17); two chromosome gradients from the MSB-1 lymphoid line (15) were examined, and similar results were obtained with both. The results presented in Fig.…”

mentioning

confidence: 66%

“…Each chromosome fraction (1 ml each of fractions 8 to 21, Fig. 1), prepared as previously described (10,17) Filters were then hybridized with specific v-erb-A and verb-B probes (29). The results are shown in Fig.…”

mentioning

confidence: 99%

“…The presence of two transduced genes in a single retroviral genome poses unique problems for current models of how transduction occurs and raises the possibility that the genes are linked within the cell of origin (1). We therefore determined the chromosomal locations of c-erb-A and c-erb-B by using fractionated chicken chromosomes (10,17) and molecular probes specific for these cellular genes.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Cellular oncogenes (c-erb-A and c-erb-B) located on different chicken chromosomes can be transduced into the same retroviral genome.

Symonds¹,

Stubblefield²,

Guyaux³

et al. 1984

Mol. Cell. Biol.

View full text Add to dashboard Cite

Avian erythroblastosis virus has transduced two cellular genes, c-erb-A and c-erb-B. Using fractionated chicken chromosomes, we found that the two genes are located on different chromosomes in the chicken genome: c-erb-A is on a microchromosome, and c-erb-B is on a large chromosome. The locations of two other cellular oncogenes (c-fps and c-myb) were also determined: c-fps is on a microchromosome, and c-myb is on chromosome of an intermediate size. Our results suggest that avian erythroblastosis virus had transduced the two cellular genes independently, conforming to previous indications that cellular oncogenes are dispersed among multiple chromosomes in every species that has been examined.

show abstract

mentioning

confidence: 66%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Cellular oncogenes (c-erb-A and c-erb-B) located on different chicken chromosomes can be transduced into the same retroviral genome.

Symonds¹,

Stubblefield²,

Guyaux³

et al. 1984

Mol. Cell. Biol.

View full text Add to dashboard Cite

show abstract

“…It is remarkable that the introduction into the cell of a single protein, the product of the src gene, can induce a new steady state. In this connection, it is of great interest that the genome of normal chicken cells contains a gene that is substantially, but apparently not completely, homologous to the DNA that is complementary to the src gene of the viral RNA (29)(30)(31). This suggests that, if the src gene product is indeed a protein kinase, it is closely related to a normal cell protein kinase.…”

Section: Discussionmentioning

confidence: 99%

Reversion of transformed glycolysis to normal by inhibition of protein synthesis in rat kidney cells infected with temperature-sensitive mutant of Rous sarcoma virus.

Carroll¹,

Ash²,

Vogt³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Normal rat kidney cells infected with a temperature-sensitive mutant (LA23) of Rous sarcoma virus exhibit the transformed~phenotype when grown at 330 and the normal phenotype at 39 . We have previously shown [Ash, J. F., Vogt, P. K. & Singer, S. J. (1976) Proc. NatL. Acad. Sci. USA 73, [3603][3604][3605][3606][3607] In a previous study (1), we investigated certain of the characteristics of malignant transformation in a particularly favorable experimental system, utilizing a normal rat kidney (NRK) cell line infected with a temperature-sensitive mutant (LA23) of Rous sarcoma virus (2). At 330 (the permissive temperature) these infected cells exhibit the transformed phenotype, and at 390 (the nonpermissive temperature) the cells appear normal; they can be rapidly switched (within hours) from one state to the other by changing the growth temperature. With such cells, we found (1) that certain structural features of the transformed phenotype would revert to normal if the cells were grown at the permissive temperature in the presence of inhibitors of protein synthesis. These structural characteristics included overall cell morphology, the degree of organization of intracellular myosin filaments, and the mobilities of concanavalin A receptors in the cell surface. These results seemed to us of great interest because they indicated not only that protein synthesis was required to maintain the transformed state in these cells but also that protein synthesis was not required to revert from the transformed to the normal phenotype, at least with respect to structural characteristics (i.e., some of the components involved in determining those structural characteristics might be altered, but not irreversibly, upon transformation). There is substantial evidence that transformation by the Rous sarcoma virus is effected by the product of a single viral gene, the src gene (3). We therefore suggested that our results were consistent with the hypothesis that the src gene product "is directly or indirectly involved in a reversibleThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement

show abstract

“…In view of current interest in protein-DNA interactions (Igo-Kemenes et at., 1982) and evidence that the site of integration of virus DNA sequences may influence the outcome of virus infection (Neel et al, 1981 ;Payne et al, 198 I), it would seem that chromosomal transfer will continue to be a valuable experimental tool, particularly in conjunction with new techniques for chromosome fractionation (Carrano J.G. LEVIN AND OTHERS et al, 1979;Padgett et al, 1977) and detection of unique DNA sequences by in situ hybridization (Tereba et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Transfer of Murine Leukaemia and Murine Sarcoma Virus Genetic Information by Transfection with Isolated Metaphase Chromosomes

Levin

Hughes²,

Graeter

et al. 1982

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYChromosome-mediated transfer of murine leukaemia (MuLV) and murine sarcoma (MuSV) virus genetic information to uninfected recipient cells was investigated. Metaphase chromosomes from AKR MuLV-infected SC-1 mouse cells were incubated with NIH/3T3 cells. After several passages (1 to 3 weeks), infectious virions exhibiting reverse transcriptase activity and the characteristic host range of ecotropic, N-tropic AKR virus appeared in the supernatant fluids of the treated cells. Restriction endonuclease analysis of genomic DNA from transfected cells indicated that AKR proviral DNA was associated with the high molecular weight DNA of the host. These results demonstrate that the AKR MuLV genome can be stably transferred to uninfected recipient cells via isolated metaphase chromosomes. Although AKR virions are not able to infect heterologous cells, chromosomemediated transfection resulted in the establishment of productive AKR MuLV infection in mink cells. Thus, the use of chromosomes to transfer virus genes can circumvent the natural host restriction barrier. In other experiments, it was shown that normal NIH/3T3 cells were transformed after exposure to metaphase chromosomes isolated from an MuSV-infected, non-producer line. Foci were detected 14 to 21 days after chromosome treatment and were shown to contain true viral transformants since transforming virus was produced after superinfection with MuLV.

show abstract

Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV

Cited by 75 publications

References 28 publications

Cellular oncogenes (c-erb-A and c-erb-B) located on different chicken chromosomes can be transduced into the same retroviral genome.

Cellular oncogenes (c-erb-A and c-erb-B) located on different chicken chromosomes can be transduced into the same retroviral genome.

Reversion of transformed glycolysis to normal by inhibition of protein synthesis in rat kidney cells infected with temperature-sensitive mutant of Rous sarcoma virus.

Transfer of Murine Leukaemia and Murine Sarcoma Virus Genetic Information by Transfection with Isolated Metaphase Chromosomes

Contact Info

Product

Resources

About