Cellular oncogenes (c-erb-A and c-erb-B) located on different chicken chromosomes can be transduced into the same retroviral genome.

Abstract

Avian erythroblastosis virus has transduced two cellular genes, c-erb-A and c-erb-B. Using fractionated chicken chromosomes, we found that the two genes are located on different chromosomes in the chicken genome: c-erb-A is on a microchromosome, and c-erb-B is on a large chromosome. The locations of two other cellular oncogenes (c-fps and c-myb) were also determined: c-fps is on a microchromosome, and c-myb is on chromosome of an intermediate size. Our results suggest that avian erythroblastosis virus had transduced the two cellular genes independently, conforming to previous indications that cellular oncogenes are dispersed among multiple chromosomes in every species that has been examined.

Cytogenetic mapping of 31 functional genes on chicken chromosomes by direct R-banding FISH

“…4). HISA@ and EGFR had previously been mapped cytogenetically (Symonds et al, 1984;Crittenden et al, 1993;ChickGBASE, January 1999). They were precisely localized to R-banded chromosomes in this study.…”

Transformation by v-Myb

“…As a result, the virus encodes a single 135 kDa Gag-Myb-Ets fusion protein. The cmyb and c-ets proto-oncogenes are present on two di erent chicken chromosomes, implying that either two successive recombinations with cellular DNA occurred or that the virus captured a pre-existing chromosomal translocation (Symonds et al, 1984b). The portion of c-Myb encoded by the E26 virus is a subset of that encoded by AMV (Nunn et al, 1984).…”

Dispersed chromosomal localization of the proto-oncogenes transduced into the genome of Mill Hill 2 or E26 leukemia virus

“…We chose to use rate zonal centrifugation to separate chromo-somes. Chromosomes from the chicken genome were fractionated on a sucrose gradient as previously described (13,24,34). Two chromosome gradients from the MSB-1 lymphoid line (22) were examined, and similar results were obtained with both.…”