Cytogenetic mapping of 31 functional genes on chicken chromosomes by direct R-banding FISH

Suzuki, Tomohiro; Kurosaki, Tomohiro; Shimada, Kiyoshi; Kansaku, Norio; Kühnlein, U.; Zadworny, D.; Agata, Kiyokazu; Hashimoto, Ari; Koide, Masafumi; Koike, Manabu; Takata, Minoru; Kuroiwa, Asato; Minai, S.; Namikawa, Takao; Matsuda, Yoichi

doi:10.1159/000015388

Cited by 53 publications

(49 citation statements)

References 41 publications

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“…The replication R-banded chromosomes were prepared following Suzuki et al (1999), and used for chromosome painting, Ag-NOR staining and chromosome mapping of DNA clones.…”

Section: Chromosome Bandingmentioning

confidence: 99%

“…The karyotypic similarities and differences between bird species have been studied morphologically by conventional Giemsa staining and chromosome banding, and, for the past 10 years, molecular cytogenetically by comparative FISH mapping with chromosome-specific paints, cDNA and genomic DNA clones, mostly developed in chicken (Gallus gallus) , Suzuki et al 1999, Schmid et al 2000, Shibusawa et al 2001. Cross-species chromosome hybridization (termed Zoo-FISH) and subsequent comparative gene mapping delineates accurately the chromosomal orthologies between distantly related species and the chromosome rearrangements that have occurred during evolution.…”

Section: Introductionmentioning

confidence: 99%

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The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

et al. 2007

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Palaeognathous birds (Struthioniformes and Tinamiformes) have morphologically conserved karyotypes and less differentiated ZW sex chromosomes. To delineate interspecific chromosome orthologies in palaeognathous birds we conducted comparative chromosome painting with chicken (Gallus gallus, GGA) chromosome 1-9 and Z chromosome paints (GGA1-9 and GGAZ) for emu, double-wattled cassowary, ostrich, greater rhea, lesser rhea and elegant crested tinamou. All six species showed the same painting patterns: each probe was hybridized to a single pair of chromosomes with the exception that the GGA4 was hybridized to the fourth largest chromosome and a single pair of microchromosomes. The GGAZ was also hybridized to the entire region of the W chromosome, indicating that extensive homology remains between the Z and W chromosomes on the molecular level. Comparative FISH mapping of four Z-and/or W-linked markers, the ACO1/IREBP, ZOV3 and CHD1 genes and the EE0.6 sequence, revealed the presence of a small deletion in the proximal region of the long arm of the W chromosome in greater rhea and lesser rhea. These results suggest that the karyotypes and sex chromosomes of palaeognathous birds are highly conserved not only morphologically, but also at the molecular level; moreover, palaeognathous birds appear to retain the ancestral lineage of avian karyotypes.2

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“…The replication R-banded chromosomes were prepared following Suzuki et al (1999), and used for chromosome painting, Ag-NOR staining and chromosome mapping of DNA clones.…”

Section: Chromosome Bandingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

et al. 2007

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“…Preparation of R-banded chromosomes and FISH were performed as described previously (Matsuda & Chapman 1995;Suzuki et al 1999).…”

Section: Chromosome Preparation and Fishmentioning

confidence: 99%

“…Mapping information of chicken genes was based on the previous studies (Suzuki et al 1999;Guttenbach et al 2000;Schmid et al 2000) and Ensembl.…”

Section: Gene Mapping Information For Human Mouse and Chicken Chromomentioning

confidence: 99%

cDNA-based gene mapping and GC3 profiling in the soft-shelled turtle suggest a chromosomal size-dependent GC bias shared by sauropsids

Kuraku¹,

Ishijima

Nishida‐Umehara

et al. 2006

Chromosome Res

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Mammalian and avian genomes comprise several classes of chromosomal segments that vary dramatically in GC-content. Especially in chicken, microchromosomes exhibit a higher GC-content and a higher gene density than macrochromosomes.To understand the evolutionary history of the intra-genome GC heterogeneity in amniotes, it is necessary to examine the equivalence of this GC heterogeneity at the nucleotide level between these animals including reptiles, from which birds diverged. We isolated cDNAs for 39 protein-coding genes from the Chinese soft-shelled turtle, Pelodiscus sinensis, and performed chromosome mapping of 31 genes. The GC-content of exonic third positions (GC 3 ) of P. sinensis genes showed a heterogeneous distribution, and exhibited a significant positive correlation with that of chicken and human orthologs, indicating that the last common ancestor of extant amniotes had already established a GC-compartmentalized genomic structure. Furthermore, chromosome mapping in P. sinensis revealed that microchromosomes tend to contain more GC-rich genes than GC-poor genes, as in chicken. These results illustrate two modes of genome evolution in amniotes: mammals sophisticated the genomic configuration in which GC-rich and GC-poor regions coexist in individual chromosomes, whereas sauropsids (reptiles and birds) refined the chromosomal size-dependent GC compartmentalization in which GC-rich genomic fractions tend to be confined to microchromosomes.2

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“…Five genes (TH, INS, CCND1, PAX6, RAG2) that lie in the p15-q13 region of human chromosome 11 have been mapped to this region of chicken chromosome 5 by in situ hybridization [23,24]. Whereas TH, INS, and CCND1 have been mapped to 11p15 and to mouse chromosome 7, PAX6 and RAG2 lie in 11p13 and on mouse chromosome 2 (Mouse Genome Database, 2001).…”

Section: Discussionmentioning

confidence: 99%

Structure and Chromosomal Localization of Chicken CD5

Koskinen¹,

Salomonsen

Goodchild

et al. 2001

Scand J Immunol

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CD5 is a transmembrane glycoprotein on all T cells and on a subpopulation of B cells. Based on the analysis of chicken CD5-cDNA we have previously shown that the structure of the CD5 protein is conserved between species. Here we report the isolation and chromosomal mapping of the chicken CD5 gene. The gene spans 3.4 kb and is extremely compact with a high GC-nucleotide content. There are 10 exons and the introns are spliced out similarly to those in the human CD5 gene. Each of the three extracellular scavenger receptor cysteine-rich (SRCR) domains is encoded as an exon of its own, as is the proline-rich hinge region that separates the first two membrane-distal SRCR domains. The fluorescence in situ hybridization (FISH) technique was used to map the gene to chromosome five. This is the first report describing the organization of the CD5 gene from a nonmammalian species.

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Cytogenetic mapping of 31 functional genes on chicken chromosomes by direct R-banding FISH

Cited by 53 publications

References 41 publications

The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds

cDNA-based gene mapping and GC3 profiling in the soft-shelled turtle suggest a chromosomal size-dependent GC bias shared by sauropsids

Structure and Chromosomal Localization of Chicken CD5

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