Chicken IgY: utilizing the evolutionary advantage

Karlsson, Marie; Kollberg, H; Larsson, Anders

doi:10.1079/wps200422

Cited by 61 publications

(58 citation statements)

References 29 publications

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“…Furthermore, in the event that entirely new viral strains appear, IgY can be produced within 6 weeks of vaccinating the hens. Finally, egg IgY is well tolerated in humans and has been used for long-term treatments of oral and intestinal pathogens without any serious side effects (5,6). Thus, this approach has great potential in helping to control highly pathogenic influenza viruses at the source of infection before they spread worldwide and cause a pandemic.…”

Section: Discussionmentioning

confidence: 99%

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice

Wallach¹,

Webby²,

Islam³

et al. 2011

Clin. Vaccine Immunol.

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Influenza viruses remain a major threat to global health due to their ability to undergo change through antigenic drift and antigenic shift. We postulated that avian IgY antibodies represent a low-cost, effective, and well-tolerated approach that can easily be scaled up to produce enormous quantities of protective antibodies. These IgY antibodies can be administered passively in humans (orally and intranasally) and can be used quickly and safely to help in the fight against an influenza pandemic. In this study, we raised IgY antibodies against H1N1, H3N2, and H5N1 influenza viruses. We demonstrated that, using whole inactivated viruses alone and in combination to immunize hens, we were able to induce a high level of anti-influenza virus IgY in the sera and eggs, which lasted for at least 2 months after two immunizations. Furthermore, we found that by use of in vitro assays to test for the ability of IgY to inhibit hemagglutination (HI test) and virus infectivity (serum neutralization test), IgYs inhibited the homologous as well as in some cases heterologous clades and strains of viruses. Using an in vivo mouse model system, we found that, when administered intranasally 1 h prior to infection, IgY to H5N1 protected 100% of the mice against lethal challenge with H5N1. Of particular interest was the finding that IgY to H5N1 cross-protected against A/Puerto Rico/8/34 (H1N1) both in vitro and in vivo. Based on our results, we conclude that anti-influenza virus IgY can be used to help prevent influenza virus infection.

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Section: Discussionmentioning

confidence: 99%

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice

Wallach¹,

Webby²,

Islam³

et al. 2011

Clin. Vaccine Immunol.

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“…Os anticorpos são moléculas com elevada especificidade para a ligação e inativação de substâncias externas, tais como moléculas tóxicas ou antígenos, que podem penetrar no organismo de animais vertebrados (Karlsson et al 2004). Em razão disto, tem sido utilizados para fins diagnósticos, terapêuticos e inclusive para a inativação de substâncias (Reilly et al 1996).…”

Section: Introductionunclassified

Produção e purificação de imunoglobulinas Y policlonais anti-Leptospira spp.

et al. 2013

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Objetivou-se verificar se galinhas imunizadas com uma solução de Leptospira interrogans inativadas e proteínas de membrana externa do sorovar Hardjo, poderiam produzir anticorpos policlonais específicos anti-leptospiras, detectáveis em testes ELISA. Foram imunizados oito galinhas com 25 semanas de idade, da raça White Leghorn, sendo três imunizadas com uma suspensão de leptospiras inativadas, três com uma solução de proteínas de membrana externa extraída do sorovar Hardjo e duas controle. Coletas de sangue foram realizadas quinzenalmente e de ovos diariamente. A IgY foi purificada a partir da gema dos ovos utilizando para a delipidação o método de diluição em água ácida e a precipitação com sulfato de amônio. Nos testes ELISA realizados para verificar a especificidade da IgY, foi demonstrada a produção de anticorpos anti-Leptospira, tanto no soro quanto nas gemas purificadas. O pico de produção de anticorpos específicos ocorreu na 5º semana após a primeira imunização. Ficou demonstrada a possibilidade da indução da produção de anticorpos específicos em galinhas imunizadas com leptospiras do sorovar Hardjo inativadas, bem como, com proteínas de membrana externa (PME) extraidas desse sorovar. As galinhas imunizadas com uma suspensão de leptospiras inativadas ou com PME de Leptospira interrogans do sorovar Hardjo produziram anticorpos reativos a PME Hardjo detectáves por teste ELISA.

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“…However, poultry is more cost-effective to produce antibody from egg yolk (100-150 mg per egg -1 ) than from blood of mammals (Karlsson et al, 2004;Ko & Ahn, 2007). In mammalian, the IgG is the most abundant than other four antibodies including IgA, IgM, IgE and IgD.…”

Section: Introductionmentioning

confidence: 99%

“…With application of mammalian IgG in disease diagnosis or experiments e.g. ELISA, detoxification of toxic materials, and prevention and treatment of diseases (Reilly et al, 1997;Yokoyama et al, 1992), IgY can prevent and cure intestinal infectious diseases such as enterotoxigenic E. coli and Salmonella (Yolken et al, 1988;Ikemori et al, 1992;Karlsson et al, 2004). Therefore, IgY is a potential substitute for antibiotics or vaccine.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Purification of Anti-Helicobacter pylori IgY

Siriya¹,

Chu²,

Chen³

et al. 2013

JAS

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The aim of this study was to establish an efficient procedure to produce anti-H. pylori urease IgY from the eggs of the laying hens. Four White Leghorn hens (52 weeks old) were immunized intramuscularly with the purified urease of H. pylori with aluminum hydroxide gel adjuvant. To increase the specificity and antibody titer, three boosters were given at 2-week intervals following the first injection. After the final immunization, the eggs were collected daily and stored at 4°C. The yield of yolk proteins were 47.52%, 41.07% and 51.35% for the methods of dextran sulphate, isopropanol and water-soluble fraction (WSF), respectively. Further, IgY was purified successively by ammonia sulfate, affinity chromatography and ion exchange chromatography for each crude protein. Ammonia sulfate salting produced the IgY with a purity of 37-53% and the titer of 22.6-29.5 unit/mg. The purity, relative purification folds and titer characteristics of the purified IgY were 85-90%, 19.63-34.61 and 223.8-273.4 unit/mg protein for affinity chromatography, and 89-92%, 25.33-47.14 and 288.8-372.4 unit/mg protein for ion exchange chromatography, respectively. This anti-Helicobacter pylori IgY was specific against H. pylori usease. Here, we established a simple and inexpensive method to obtain a yield of 90% purity of the anti-H. pylori IgY in combinations of the WSF method, ammonia sulfate precipitation, and affinity chromatography.

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Chicken IgY: utilizing the evolutionary advantage

Cited by 61 publications

References 29 publications

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice

Produção e purificação de imunoglobulinas Y policlonais anti-Leptospira spp.

Extraction and Purification of Anti-Helicobacter pylori IgY

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