Chick NKx-2.3 represents a novel family member of vertebrate homologues to the Drosophila homeo☐ gene tinman: differential expression of cNKx-2.3 and cNKx-2.5 during heart and gut development

Buchberger, Astrid; Pabst, Oliver; Brand, Thomas; Seidl, Klaus; Arnold, Hans-Henning

doi:10.1016/0925-4773(96)00521-7

Cited by 76 publications

(33 citation statements)

References 45 publications

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“…Nkx-2.5 RNA has previously been shown to be expressed in the embryonic chick heart up to the looped, tubular stage (HH 15-17) (Schultheiss et al, 1995;Buchberger et al, 1996). During later development, we found that a heterogeneous pattern of Nkx-2.5 expression emerged (Figs.…”

Section: Nkx-25 Mrna Is Significantly Elevated In the Central Conducsupporting

confidence: 53%

Elevated expression of Nkx‐2.5 in developing myocardial conduction cells

et al. 2001

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A number of different phenotypes emerge from the mesoderm-derived cardiomyogenic cells of the embryonic tubular heart, including those comprising the cardiac conduction system. The transcriptional regulation of this phenotypic divergence within the cardiomyogenic lineage remains poorly characterized. A relationship between expression of the transcription factor Nkx-2.5 and patterning to form cardiogenic mesoderm subsequent to gastrulation is well established. Nkx-2.5 mRNA continues to be expressed in myocardium beyond the looped, tubular heart stage. To investigate the role of Nkx-2.5 in later development, we have determined the expression pattern of Nkx-2.5 mRNA by in situ hybridization in embryonic chick, fetal mouse, and human hearts, and of Nkx-2.5 protein by immunolocalization in the embryonic chick heart. As development progresses, significant nonuniformities emerge in Nkx-2.5 expression levels. Relative to surrounding forcegenerating ("working") myocardium, elevated Nkx-2.5 mRNA signal becomes apparent in the specialized cells of the conduction system. Similar differences are found in developing chick, human, and mouse fetal hearts, and nuclear-localized Nkx-2.5 protein is prominently expressed in differentiating chick conduction cells relative to adjacent working myocytes. This tissue-restricted expression of Nkx-2.5 is transient and correlates with the timing of spatio-temporal recruitment of cells to the central and the peripheral conduction system. Our data represent the first report of a transcription factor showing a stage-dependent restriction to different parts of the developing conduction system, and suggest some commonality in this development between birds and mammals. This dynamic pattern of expression is consistent with the hypothesis that Nkx-2.5, and its level of expression, have a role in regulation and/or maintenance of specialized fate selection by embryonic myocardial cells.

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Section: Nkx-25 Mrna Is Significantly Elevated In the Central Conducsupporting

confidence: 53%

Elevated expression of Nkx‐2.5 in developing myocardial conduction cells

et al. 2001

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“…In vitro transcribed and translated proteins were generated by using TNTcoupled reticulocyte lysate systems (Promega). 1 l of reticulocyte lysate containing 35 S-labeled wild type or mutant Csx/Nkx2.5, Nkx2.3, or Nkx2.6/Tix protein was mixed with fusion proteins in a 400 l of binding buffer (20 mM HEPES, pH 7.5, 100 mM NaCl, 5 mM MgCl 2 , 0.1% Triton X-100, aprotinin (2 g/ml), pepstatin (0.7 g/ml), 0.1 mM PMSF, 1 mM DTT, and 1% bovine serum albumin) at 4°C for 2 h. Beads were washed with binding buffer (without bovine serum albumin) and subjected to SDS-PAGE.…”

Section: Methodsmentioning

confidence: 99%

“…After extensive washing, the protein complexes were resolved on SDS-PAGE and visualized by autoradiography. Approximately 25% of input 35 S-labeled Csx/ Nkx2.5 protein associated with MBP-Csx/Nkx2.5 ( Fig. 2A, lane …”

Section: Csx/nkx25 Forms a Homodimer On A Palindromic Dnamentioning

confidence: 99%

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Characterization of Homo- and Heterodimerization of Cardiac Csx/Nkx2.5 Homeoprotein

Kasahara¹,

Usheva²,

Ueyama³

et al. 2001

Journal of Biological Chemistry

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“…Nkx2.5, the vertebrate homolog of the Drosophila tinman gene, is expressed in the cardiac lineage of fish, chick, and mouse (Chen and Fishman, 1996;Buchberger et al, 1996;Lints et al, 1993). It is essential for myogenic and morphogenetic differentiation in the mammalian heart (Lyons et al, 1995).…”

Section: Bmp4 Msx1 and Nkx25 Expression During Oft And Av Cushionsmentioning

confidence: 99%

Characterization of molecular markers to assess cardiac cushions formation in Xenopus

Lee

Saint-Jeannet

2009

Developmental Dynamics

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The valves and septa of the mature heart are derived from the cardiac cushions, which develop from discrete swellings in two regions of developing heart tube: the atrioventricular (AV) canal and the ventricular outflow tract (OFT). In higher vertebrates, three distinct lineages contribute to the heart valves and septa, the endocardium, the myocardium, and the cardiac neural crest that will populate the cardiac jelly of the OFT. Very little is known about cardiac cushions development in amphibians. Here, we describe the expression of eight genes during key stages of cardiac cushion development in Xenopus. Among these genes, the Wnt antagonist Frzb1 and the transcription factors Xl-Fli, Sox8, Sox9, and Sox10 are differentially expressed in the mesenchyme of the OFT and AV cushions. These genes can be used in combination with lineage-tracing experiments to determine the embryonic origin of the cardiac cushions mesenchyme in Xenopus. Developmental Dynamics 238:3257-3265,

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Chick NKx-2.3 represents a novel family member of vertebrate homologues to the Drosophila homeo☐ gene tinman: differential expression of cNKx-2.3 and cNKx-2.5 during heart and gut development

Cited by 76 publications

References 45 publications

Elevated expression of Nkx‐2.5 in developing myocardial conduction cells

Elevated expression of Nkx‐2.5 in developing myocardial conduction cells

Characterization of Homo- and Heterodimerization of Cardiac Csx/Nkx2.5 Homeoprotein

Characterization of molecular markers to assess cardiac cushions formation in Xenopus

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