Chick Chorioallantoic Membrane as an In Situ Biological Membrane for Pharmaceutical Formulation Development: A Review

Saw, Constance Lay Lay; Heng, Paul Wan Sia; Liew, Celine Valeria

doi:10.1080/03639040801974295

Cited by 29 publications

(14 citation statements)

References 34 publications

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“…Originally developed to investigate angiogenesis, the CAM assay evolved to an accepted and reliable in vivo model to replace animal experiments for testing different substances and chemotherapeutics [34][35][36]. Toxicity of cnicin for the embryo was tested (data not shown) and cancer xenografts were then treated with methanol (as a solvent control) or with cnicin at nonlethal concentrations and pictures were taken after 6 days growth.…”

Section: Cnicin Efficiently Kills Myeloma Cells In An Ex Ovo Cam Assaymentioning

confidence: 99%

Antimyeloma activity of the sesquiterpene lactone cnicin: impact on Pim-2 kinase as a novel therapeutic target

et al. 2011

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Despite recent advances in therapy, multiple myeloma, the second most common hematologic tumor in the Western world, is still incurable. Identification of substances that display a wide range of tumor-killing activities and target cancer-specific pathways constitute a basis for the development of novel therapies. In this study, we investigate the cytotoxic effect of the natural substance cnicin in multiple myeloma. Cnicin treatment reveals potent antiproliferative effects and induces cell death in cell lines and primary myeloma cells even in the presence of survival cytokines and the tumor microenvironment. Other cell lines of hematopoietic origin also succumb to cell death whereas stromal cells and endothelial cells are unaffected. We show that activation of caspases, accumulation of reactive oxygen species and downregulation of nuclear factor kappa-light-chain-enhancer of activated B cell contribute to the cytotoxic effects of cnicin. Microarray analysis reveals downregulation of Pim-2, a serine/threonine kinase. We provide evidence that Pim-2 constitutes a new survival kinase for myeloma cells in vitro and is highly expressed in malignant but not in normal plasma cells in vivo. Combining cnicin with current standard or experimental therapeutics leads to enhanced cell death. Thus, our data indicate that cnicin induces myeloma cell death via several pathways and reveals Pim-2 as a novel target. These findings provide a rational for further evaluation of cnicin as a new anti-tumor drug and underline the potential of sesquiterpene lactones in tumor therapy.

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Section: Cnicin Efficiently Kills Myeloma Cells In An Ex Ovo Cam Assaymentioning

confidence: 99%

Antimyeloma activity of the sesquiterpene lactone cnicin: impact on Pim-2 kinase as a novel therapeutic target

et al. 2011

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“…Besides, SAs could also enhance angiogenesis in CAM model. The use of CAM was one of the proposed alternatives of testing models to mimic human tissue with several advantages including simplicity, rapidity, sensitivity, ease of performance, and cost-effectiveness (Saw et al, 2008). Given the well-established role of EPCs participating in neovascularization and reendothelialization, the results from this study thus indicate that SAs may have utility for therapeutic postnatal vasculogenesis of ischemic tissue, limiting neointimal formation and ultimately the occlusion of diseased vessels in part by enhancing the mobilization and homing of EPCs after vascular injury, contribute to the clinical benefit of SM therapy in patient with CAD.…”

Section: Discussionmentioning

confidence: 99%

Pro-angiogenic actions of Salvianolic acids on in vitro cultured endothelial progenitor cells and chick embryo chorioallantoic membrane model

Duan

Liu

et al. 2010

Journal of Ethnopharmacology

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“…107 109 In addition, the CAM was initially used as an alternative to the Draize test, which originally involved exposing the eyes of rabbits to chemicals to determine irritation. 110 The CAM has a comparable sensitivity to a rabbit's eye, yet the embryo feels no pain until late in its gestation; therefore, this was a much more effective and humane way to test chemical irritation. Not only does the highly vascularized nature of the CAM allow researchers to study angiogenic-related disease, it is also relatively immunodeficient in comparison to other in vivo models.…”

Section: Chorioallantoic Membrane (Cam)mentioning

confidence: 99%

“…Over the years, the CAM model has evolved from simply exposing drugs, tumor cells, and potential vaccines to the surface of the CAM to researchers using complex injection and imaging techniques. [123][124][125] The CAM model has also evolved over time to be used to study anti-tumor drugs and further to study the targeted delivery of said drugs. 126 127 Here, we will briefly discuss the background of the study of angiogenesis in the CAM and antitumor drugs and how that has led to the study of NPs used for drug delivery.…”

Section: In Ovo Versus Ex Ovo Assaymentioning

confidence: 99%

Testing Nanoparticles for Angiogenesis-Related Disease: Charting the Fastest Route to the Clinic

Sarsons¹,

Tekrony²,

Yaehne³

et al. 2014

j biomed nanotechnol

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Recently, nanoparticles (NPs) have been established as ideal drug delivery vehicles for treating cancer. This is due to the enhanced permeability and retention (EPR) effect that is a direct result of the angiogenic nature of the tumor tissue and its ability to sequester chemotherapeutics from healthy tissues. Ideal drug delivery nanocarriers will exploit the EPR effect, accumulate in the tumorous tissue, and be able to release the drugs at a high concentration where needed, thereby reducing undesirable side effects. In order to determine ideal NP qualities that enable drugs to be delivered in such a manner, extensive testing in biological systems is required. However, it is impractical to study new potential nanocarriers in humans or in mammalian models due to the potential adverse consequences, low throughput, and high cost. Simpler models would allow for higher throughput screening of nanocarrier vehicles. This review outlines the most recent advances in alternative model assays and their significance in testing NPs en route to the clinic. In decreasing complexity, we examine zebrafish embryos, the chorioallantoic membrane of the chicken embryo, multicell static and flow-based assays, and single cell assays for efficacy, accuracy and utility as predictors for human therapeutic outcomes.

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Chick Chorioallantoic Membrane as an In Situ Biological Membrane for Pharmaceutical Formulation Development: A Review

Cited by 29 publications

References 34 publications

Antimyeloma activity of the sesquiterpene lactone cnicin: impact on Pim-2 kinase as a novel therapeutic target

Antimyeloma activity of the sesquiterpene lactone cnicin: impact on Pim-2 kinase as a novel therapeutic target

Pro-angiogenic actions of Salvianolic acids on in vitro cultured endothelial progenitor cells and chick embryo chorioallantoic membrane model

Testing Nanoparticles for Angiogenesis-Related Disease: Charting the Fastest Route to the Clinic

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