Chemotherapy of Chlamydial Infections

Antimicrobial Activity of Several Antibiotics and a Sulfonamide Against Chlamydia trachomatis Organisms in Cell Culture

“…DISCUSSION This is the first report in which all known immunotypes of C. trachomatis were tested for drug susceptibility. The results found in this study on the antichlamydial activity of tetracycline, erythromycin, chloramphenicol, penicillin, and sulfonamide were, in general, similar to those reported in earlier studies with animal models, embryonated eggs, or cell cultures (3,6,7,11). The studies have found tetracycline and erythromycin to be the most effective, which is consistent with results of treatment of hulman infections.…”

“…While the antibiotic clearly causes abnormal growth patterns, it is difficult to eradicate the organism with a reasonable dosage. In experiments with mice, doses of penicillin that prevented death of infected mice were not effective in eradication of the organisms from the host (2,7). In embryonated eggs much larger amounts of penicillin were required to completely inhibit chlamydial replication than to protect eggs from infectious death (7).…”

“…; (vi) ampicillin, Omnipen-N (sodium ampicillin), Wyeth Laboratories, Inc., Philadelphia, Pa.; and (vii) sulfonamide, Gantrisin (sulfisoxazole diolamine), Hoffman-La Roche, Inc., Nutley, N.J. Commercial preparations were used, except for rosamicin which was used in pure form. These agents, except rosamicin, have been known to inhibit chlamydiae (3,6,7).…”

“…In this. report, the activities of six antimicrobial drugs known to be inhibitory against Chlamydia, both in natural infection and in vitro testing (7), were tested in HeLa 229 cell cultures (9) against C. trachomatis of all known immunological types. In addition, a new macrolide antibiotic, rosamicin (12), was also tested.…”

Antibiotic Susceptibility of Chlamydia trachomatis

“…These chlamydial strains were first recovered less than 20 years ago from ocular disease and later from genital tract infections. Many studies were performed in embryonated hens' eggs on the susceptibility of the organisms to chemotherapeutic agents (6,11). Since the yolk sac technique was a difficult and timeconsuming one, the introduction of the tissue culture (TC) method was a major technical advance for the isolation of these chlamydiae (3).…”