Chemotherapy control by breath profile with application of <scp>SPME</scp>‐<scp>GC</scp>/<scp>MS</scp> method

Ulanowska, Agnieszka; Trawińska, Ewa; Sawrycki, Piotr; Buszewski, Bogusław

doi:10.1002/jssc.201200333

Cited by 28 publications

(13 citation statements)

References 16 publications

(16 reference statements)

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“…Recent efforts in pursuits of a real-time on-line breath analyzer with high data throughput are fruitful [9,11,18,19,20,21,22,23,24,25,26,27,28]. Several relatively new MS-based analytical techniques have been developed and tested for on-line breath analysis, including proton transfer reaction mass spectrometry (PTR-MS) [22,23], vacuum-free ion mobility spectroscopy (IMS) [24,25], and selected ion flow tube mass spectrometry (SIFT-MS) [9,11,26].…”

Section: Introductionmentioning

confidence: 99%

A Portable Real-Time Ringdown Breath Acetone Analyzer: Toward Potential Diabetic Screening and Management

Jiang

Sun

Wang

et al. 2016

Sensors

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Breath analysis has been considered a suitable tool to evaluate diseases of the respiratory system and those that involve metabolic changes, such as diabetes. Breath acetone has long been known as a biomarker for diabetes. However, the results from published data by far have been inconclusive regarding whether breath acetone is a reliable index of diabetic screening. Large variations exist among the results of different studies because there has been no “best-practice method” for breath-acetone measurements as a result of technical problems of sampling and analysis. In this mini-review, we update the current status of our development of a laser-based breath acetone analyzer toward real-time, one-line diabetic screening and a point-of-care instrument for diabetic management. An integrated standalone breath acetone analyzer based on the cavity ringdown spectroscopy technique has been developed. The instrument was validated by using the certificated gas chromatography-mass spectrometry. The linear fittings suggest that the obtained acetone concentrations via both methods are consistent. Breath samples from each individual subject under various conditions in total, 1257 breath samples were taken from 22 Type 1 diabetic (T1D) patients, 312 Type 2 diabetic (T2D) patients, which is one of the largest numbers of T2D subjects ever used in a single study, and 52 non-diabetic healthy subjects. Simultaneous blood glucose (BG) levels were also tested using a standard diabetic management BG meter. The mean breath acetone concentrations were determined to be 4.9 ± 16 ppm (22 T1D), and 1.5 ± 1.3 ppm (312 T2D), which are about 4.5 and 1.4 times of the one in the 42 non-diabetic healthy subjects, 1.1 ± 0.5 ppm, respectively. A preliminary quantitative correlation (R = 0.56, p < 0.05) between the mean individual breath acetone concentration and the mean individual BG levels does exist in 20 T1D subjects with no ketoacidosis. No direct correlation is observed in T1D subjects, T2D subjects, and healthy subjects. The results from a relatively large number of subjects tested indicate that an elevated mean breath acetone concentration exists in diabetic patients in general. Although many physiological parameters affect breath acetone, under a specifically controlled condition fast (<1 min) and portable breath acetone measurement can be used for screening abnormal metabolic status including diabetes, for point-of-care monitoring status of ketone bodies which have the signature smell of breath acetone, and for breath acetone related clinical studies requiring a large number of tests.

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Section: Introductionmentioning

confidence: 99%

A Portable Real-Time Ringdown Breath Acetone Analyzer: Toward Potential Diabetic Screening and Management

Jiang

Sun

Wang

et al. 2016

Sensors

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“…Gas chromatographymass spectrometry (GC-MS) [5][6][7][8][9][10] has been regarded as a gold-standard method for trace gas analysis for its high sensitivity and high accuracy. However, the GC-MS method is not suitable for breath analysis using a large number of samples due to its complicated sample preparation procedure, time-consuming test process, and high instrumentation and operational costs.…”

Section: Introductionmentioning

confidence: 99%

A fully integrated standalone portable cavity ringdown breath acetone analyzer

Sun

Jiang

Gong

et al. 2015

Review of Scientific Instruments

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A laser-locked cavity ring-down spectrometer employing an analog detection scheme Review of Scientific Instruments 71, 347 (2000) Breath analysis is a promising new technique for nonintrusive disease diagnosis and metabolic status monitoring. One challenging issue in using a breath biomarker for potential particular disease screening is to find a quantitative relationship between the concentration of the breath biomarker and clinical diagnostic parameters of the specific disease. In order to address this issue, we need a new instrument that is capable of conducting real-time, online breath analysis with high data throughput, so that a large scale of clinical test (more subjects) can be achieved in a short period of time. In this work, we report a fully integrated, standalone, portable analyzer based on the cavity ringdown spectroscopy technique for near-real time, online breath acetone measurements. The performance of the portable analyzer in measurements of breath acetone was interrogated and validated by using the certificated gas chromatography-mass spectrometry. The results show that this new analyzer is useful for reliable online (online introduction of a breath sample without pretreatment) breath acetone analysis with high sensitivity (57 ppb) and high data throughput (one data per second). Subsequently, the validated breath analyzer was employed for acetone measurements in 119 human subjects under various situations. The instrument design, packaging, specifications, and future improvements were also described. From an optical ringdown cavity operated by the lab-set electronics reported previously to this fully integrated standalone new instrument, we have enabled a new scientific tool suited for large scales of breath acetone analysis and created an instrument platform that can even be adopted for study of other breath biomarkers by using different lasers and ringdown mirrors covering corresponding spectral fingerprints. C 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.[http://dx

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“…Laser magnetic resonance spectroscopy can be a simple alternative to mass spectrometry in detection of exhaled 14 C-urea in Helicobacter pylori infection [226, 227]. A method of gas chromatography combined with ultraviolet spectroscopy has been used to measure exhaled acetone and isoprene levels in diabetic school children [228] and to monitor pulmonary cancer-cytostatic chemotherapy efficacy [39]. Polymer-coated surface-acoustic-wave resonators are portable instruments which have been introduced and are able to analyze organic vapors [229].…”

Section: Discussionmentioning

confidence: 99%

“…A recent study of microsatellite alterations (MAs) of 3p in the EBC deoxyribonucleic acid (DNA) of patients with nonsmall cell pulmonary cancer revealed that MAs in EBC DNA of cancer patients were significantly more common than in normal subjects and MA profile of EBC DNA corresponded to that from cancer tissue of each patient [38]. Isoprene and acetone were shown to increase in the exhaled breath measured by SPME-GC-MS breath test after cytostatic chemotherapy indicating the usefulness of this technique in monitoring of pulmonary cancer-chemotherapy efficacy [39]. Some recent studies revealed reduction of mutated KRAS oncogene in EBC [40] and some VOCs in exhaled breath [41] after surgical resection of pulmonary tumor, particularly nonsmall cell type.…”

Section: Exhaled Markers In Pulmonary Cancermentioning

confidence: 99%

Breath Tests in Respiratory and Critical Care Medicine: From Research to Practice in Current Perspectives

Cheepsattayakorn

2013

BioMed Research International

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Today, exhaled nitric oxide has been studied the most, and most researches have now focusd on asthma. More than a thousand different volatile organic compounds have been observed in low concentrations in normal human breath. Alkanes and methylalkanes, the majority of breath volatile organic compounds, have been increasingly used by physicians as a novel method to diagnose many diseases without discomforts of invasive procedures. None of the individual exhaled volatile organic compound alone is specific for disease. Exhaled breath analysis techniques may be available to diagnose and monitor the diseases in home setting when their sensitivity and specificity are improved in the future.

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Chemotherapy control by breath profile with application of SPME‐GC/MS method

Cited by 28 publications

References 16 publications

A Portable Real-Time Ringdown Breath Acetone Analyzer: Toward Potential Diabetic Screening and Management

A Portable Real-Time Ringdown Breath Acetone Analyzer: Toward Potential Diabetic Screening and Management

A fully integrated standalone portable cavity ringdown breath acetone analyzer

Breath Tests in Respiratory and Critical Care Medicine: From Research to Practice in Current Perspectives

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