A fully integrated standalone portable cavity ringdown breath acetone analyzer

Abstract

A laser-locked cavity ring-down spectrometer employing an analog detection scheme Review of Scientific Instruments 71, 347 (2000) Breath analysis is a promising new technique for nonintrusive disease diagnosis and metabolic status monitoring. One challenging issue in using a breath biomarker for potential particular disease screening is to find a quantitative relationship between the concentration of the breath biomarker and clinical diagnostic parameters of the specific disease. In order to address this issue, we need a new instrument that is capable of conducting real-time, online breath analysis with high data throughput, so that a large scale of clinical test (more subjects) can be achieved in a short period of time. In this work, we report a fully integrated, standalone, portable analyzer based on the cavity ringdown spectroscopy technique for near-real time, online breath acetone measurements. The performance of the portable analyzer in measurements of breath acetone was interrogated and validated by using the certificated gas chromatography-mass spectrometry. The results show that this new analyzer is useful for reliable online (online introduction of a breath sample without pretreatment) breath acetone analysis with high sensitivity (57 ppb) and high data throughput (one data per second). Subsequently, the validated breath analyzer was employed for acetone measurements in 119 human subjects under various situations. The instrument design, packaging, specifications, and future improvements were also described. From an optical ringdown cavity operated by the lab-set electronics reported previously to this fully integrated standalone new instrument, we have enabled a new scientific tool suited for large scales of breath acetone analysis and created an instrument platform that can even be adopted for study of other breath biomarkers by using different lasers and ringdown mirrors covering corresponding spectral fingerprints. C 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.[http://dx

Study of Breath Acetone in a Rat Mode of 126 Rats with Type 1 Diabetes

“…The detailed information on the instrument design, validation, and measuring principle can be seen elsewhere [13,14]. Briefly, a 266 nm laser source (Changchun New Industries Optoelectronics, MPL-F-266 nm, China), two plane mirrors (Thorlabs, PF05-03-F01, U.S.), a gas cell (ringdown cavity, CRD Optics Inc., U.S.), two high reflectivity plano-concave mirrors (Los Gatos Research, R=99.99%, radius of curvature=1 m), a micro pressure manometer (MKS, 870B, U.S.), and a miniature photomultiplier tube (PMT, Hamamatsu, H10721, Japan) were the major components of the instrument.…”

Temperature Programmed Desorption of Quench-condensed Krypton and Acetone in Air; Selective Concentration of Ultra-trace Gas Components

“…These are the case of, for example, trace gas analysis using quantum cascade lasers 23 and cavity ringdown spectroscopy (CRDS). 24 These are absorption spectroscopy-based techniques, and therefore, analytes are limited by the availability of probe laser light at the appropriate wavelength. Concerning the selectivity of low temperature TPD combined with QMS, more than several different components of gases with m/z smaller than 200 -300 can be simultaneously measured.…”

“…For example, recent CRDS studies reported a real-time response without a sample pre-process. 24 This is obviously superior to low temperature TPD, which needs at least a few minutes to complete one measurement.…”

Continuous Monitoring of Breath Acetone, Blood Glucose and Blood Ketone in 20 Type 1 Diabetic Outpatients Over 30 Days

“…Laser spectroscopy used for breath analysis has the advantages of high sensitivity, high selectivity, fast response, low instrumental cost, and relatively small instrument geometry as compared with the gas chromatography mass spectrometry (GC-MS) method which is an established method primarily used for lab-based trace gas analysis. Recently, several groups have developed novel optical approaches attempting to focus on a compact and practical solution for single analyte detection [20][21][22][23][24][25]. Notably, a recent paper reports on a portable and compact device for BA monitoring based upon laser cavity enhanced absorption spectroscopy [20].…”