Chemotaxonomic Study of Vibrio cholerae Bio‐Serogroup Hakata on the Basis of the Sugar Composition of the Polysaccharide Portion of Its Lipopolysaccharides

Abstract

A chemotaxonomic study was carried out on 31 strains of non-O1 Vibrio cholerae bio-serogroup Hakata, isolated in Japan, which possesses the Inaba antigen C of O1 V. cholerae. On the basis of the compositional sugar pattern of the polysaccharide portion of their lipopolysaccharides, the 23 strains isolated from the environment were separated into two groups, one (20 strains) containing mannose, glucose, fructose, L-glycero-D-mannoheptose, glucosamine, perosamine, quinovosamine, and an unidentified amino sugar AS, and the other (3 strains) containing two additional sugars, galactose and a trace amount of galactosamine. All of the eight strains isolated from imported seafoods belonged to the former group.On the basis of their heat-stable somatic antigen (or O-antigen), Vibrio cholerae is divided into O1 and non-O1 V. cholerae; the former is further subdivided into two major O-forms, Ogawa and Inaba. These two O-forms share group somatic antigen A, but can be separated by antigen B (Ogawa factor) and C (Inaba factor) (1, 7). Recently, a bio-serogroup of non-O1 Vibrio cholerae possessing a somatic antigen factor in common with 01 V. cholerae was isolated (8). Shimada and Sakazaki (8) demonstrated by cross-agglutination and cross-agglutinin absorption tests that this group, designated as V. cholerae bio-serogroup Hakata, possesses the Inaba antigen factor C of O1 V. cholerae.In our previous study, a rapid method was developed for preparing a sample for the compositional sugar analysis of the degraded polysaccharide (DPS) fraction, which corresponds almost completely to the polysaccharide portion of lipopolysaccharides (LPS), from V. cholerae cells (4). This rapid method was successfully applied to the chemotaxonomy of non-O1 V. cholerae; 43 serogroups of non-O1 V. cholerae O2 to O41, O44 and O68 were divided into 24 chemotypes on the basis of the compositional sugar pattern of the polysaccharide portion of their LPS (6).This article presents a chemotaxonomic study, on the same basis as the preceding work, of 31 strains of V. cholerae bio-serogroup Hakata isolated in Japan from the environment and imported seafoods, in which the same rapid method was used with some modification.

Structural analysis of a novel putative capsular polysaccharide from Pseudomonas (Burkholderia) caryophylli strain 2151

“…Fructose occurs relatively rarely in bacterial polysaccharides. It has been found in the LPSs of several Vibrio species [13–20] and in the K4 [21] and K11 [22] capsular antigens of Escherichia coli. In all cases, fructose appears as a terminal residue.…”

N-3-hydroxypropionyl-α-d-perosamine homopolymer constituting the O-chain of lipopolysaccharides from Vibrio bioserogroup 1875 possessing antigenic factor(s) in common with O1 Vibrio cholerae

“…Kobe [2] and non-Ol V. cholerae bioserogroup Hakata [3]. Isolation and chemical as well as serological characterizations, in particular, compositional sugar analysis, of their lipopolysaccharides (LPS) were carried out by our group [4][5][6][7]. Of particular interest is the fact that the 0-chain of LPS from non-Ol V. cholerae Hakata contains a linear (1 -+2)-linked N-acetyl-a-D-perosamine (4-acetamido-4,6-dideoxy-a-D-mannopyranose) homopolymer [7] which is very similar to, but not identical with, a linear (1 -+2)-linked N-3-deoxy-Lglycero-tetronyl(S-2,4-dihydroxybutyryl)-a-D-perosamine homopolymer which constitutes the 0-chain of 01 V. cholerae LPS [8,9].…”