Chemotactic migration of mesencephalic neural crest cells in the mouse

Expression of chondrogenic potential of mouse trunk neural crest cells by FGF2 treatment

“…The cultures were immunostained with the following primary antibodies: (1) goat anti-collagen type II (1:100; Southern Biotechnology Associates) to detect chondrogenic differentiation, (2) mouse anti-neurofilament 68KD (1:4; Boehringer) for detection of neurons, (3) rabbit anti-FGFR1 (1:100; Santa Cruz; Kubota and Ito, 2000;Sarkar et al, 2001), (4) rabbit anti-FGFR2 (1: 500; Santa Cruz; Kubota and Ito, 2000;Sarkar et al, 2001), (5) rabbit anti-FGFR3 (1:500; Santa Cruz; Kubota and Ito, 2000;Sarkar et al, 2001), and (6) rabbit anti-Hox9 (1:500; Santa Cruz) to detect the expression of the Hox9 paralogous group. This antibody has previously been used in other studies (Gattenlöhner et al, 2003;Okada et al, 2003).…”

Induction of neurogenin‐1 expression by sonic hedgehog: Its role in development of trigeminal sensory neurons

“…FGF-2 promotes the proliferation of neural crest cells (Murphy et al, 1994;Zhang et al, 1997;Sieber-Blum and Zhang, 1997;Sieber-Blum, 1998) and regulates their migration (Kubota and Ito, 2000). FGF-8 affects not only midbrain development (Crossley et al, 1996;Lee et al, 1997) but also neural crest cell migration (Kubota and Ito, 2000). NT-3 is a differentiation and survival factor for neurons (Snider, 1994) that promotes Brn3a expression in trigeminal sensory neurons (Wyatt et al, 1998).…”

“…FGF-2 promotes chondrogenesis and skeletogenesis of cranial neural crest cells ( Sarkar et al, 2001;Petiot et al, 2002). FGF-2, furthermore, acts as a chemoattractant of cranial neural crest cell migration (Kubota and Ito, 2000). Thus, FGF-2 has multiple functions in neural crest development.…”

“…Mouse cranial organ cultures were prepared by a modification of the method developed by Kubota and Ito (2000). Explants containing the posterior mesencephalon and the most-anterior rhombencephalon along with the mandibular arch of embryonic day (Ed) 9.0 (13-to 15-somite stages) ddY mouse embryos were microdissected with tungsten needles.…”

“…Immunostainings of embryos and organ culture explants were performed as described previously (Kubota et al, 1996(Kubota et al, , 1999Kubota and Ito, 2000). Embryos or explants were fixed with 4% paraformaldehyde (PFA) in phosphate-buffered saline (pH 7.4) or with methanol containing 0.1% formaldehyde at Ϫ20°C for 10 min in staining using a rat monoclonal antibody, 4E9R.…”