Expression of chondrogenic potential of mouse trunk neural crest cells by FGF2 treatment

Ido, Atsushi; K, Ito

doi:10.1002/dvdy.20635

Cited by 42 publications

(32 citation statements)

References 23 publications

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“…The present work shows in vitro chondrocyte differentiation of TNCCs, a finding described previously (53)(54)(55). Similarly to CNCCs, chondrogenesis by TNCCs is markedly increased in the presence of Shh.…”

Section: Discussionsupporting

confidence: 89%

Sonic Hedgehog promotes the development of multipotent neural crest progenitors endowed with both mesenchymal and neural potentials

Calloni

Glavieux-Pardanaud

Douarin

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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In the vertebrate embryo, the cephalic neural crest cells (CNCCs) produce cells belonging to two main lineages: the neural [including neurons, glial cells of the peripheral nervous system (PNS), and melanocytes] and the mesenchymal (chondrocytes, osteoblasts, smooth muscle cells, and connective tissue cells), whereas the trunk NCCs (TNCCs) in amniotes yield only neural derivatives. Although multipotent cells have previously been evidenced by in vitro clonal analysis, the issue as to whether all of the mesenchymal and neural phenotypes can be derived from a unique NC stem cell has remained elusive. In the present work, we devised culture conditions that led us to identify a highly multipotent NCC endowed with both neural and mesenchymal potentials, which lies upstream of all the other NC progenitors known so far. We found that addition of recombinant Sonic Hedgehog (Shh) increased the number of CNCC progenitors yielding both mesenchymal and neural lineages and promoted the development of such precursors from the TNCC. Shh decreased the neural-restricted precursors without affecting the overall CNCC survival and proliferation. By showing a differential positive effect of Shh on the expression of mesenchymal phenotypes (i.e., chondrocytes and smooth muscle cells) by multipotent CNCCs, these results shed insights on the in vivo requirement of Shh for craniofacial morphogenesis. Together with evolutionary considerations, these data also suggest that the mesenchymal-neural precursor represents the ancestral form of the NC stem cell, which in extinct forms of vertebrates (the ostracoderms) was able to yield both the PNS and superficial skeleton.chondrogenesis ͉ clonal culture ͉ embryo ͉ quail ͉ stem cell

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Section: Discussionsupporting

confidence: 89%

Sonic Hedgehog promotes the development of multipotent neural crest progenitors endowed with both mesenchymal and neural potentials

Calloni

Glavieux-Pardanaud

Douarin

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…Finally, it has also been shown that FGFs promote the formation of ectomesenchymal derivatives. Absence of FGF function results in a failure in the formation of pharyngeal cartilages (David et al, 2002;Walshe and Mason, 2003), and the culturing of neural crest cells in FGFs promotes skeletogenesis (Sarkar et al, 2001;Ido and Ito, 2006).…”

Section: Discussionmentioning

confidence: 99%

The emergence of ectomesenchyme

Blentic

Tandon

Payton

et al. 2008

Developmental Dynamics

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“…In support of this, multipotent progenitors with neural as well as mesenchymal potentials were rarely observed in clonal cell culture assays of trunk NC cells [2,3] and trunk NC was ineffective in forming cartilage after transplantation in vivo [4]. However, incubating trunk NC cells in tailor-made chondrogenesis-permissive media for a prolonged time period led to the emergence of mature chondrocytic markers [5,6]. Furthermore, mesenchymal stem cells (MSCs) originating from the NCs were recently isolated from the trunk of mouse embryos [7].…”

Section: Introductionmentioning

confidence: 89%

Transforming Growth Factor β-Mediated Sox10 Suppression Controls Mesenchymal Progenitor Generation in Neural Crest Stem Cells

et al. 2011

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During vertebrate development, neural crest stem cells (NCSCs) give rise to neural cells of the peripheral nervous system and to a variety of mesenchymal cell types, including smooth muscle, craniofacial chondrocytes, and osteocytes. Consistently, mesenchymal stem cells (MSCs) have recently been shown to derive in part from the neural crest (NC), although the mechanisms underlying MSC generation remains to be identified. Here, we show that transforming growth factor b (TGFb)-mediated suppression of the NCSC transcription factor Sox10 induces a switch in neural to mesenchymal potential in NCSCs. In vitro and in vivo, TGFb signal inactivation results in persistent Sox10 expression, decreased cell cycle exit, and perturbed generation of mesenchymal derivatives, which eventually leads to defective morphogenesis. In contrast, TGFb-mediated downregulation of Sox10 or its genetic inactivation suppresses neural potential, confers mesenchymal potential to NC cells in vitro, and promotes cell cycle exit and precocious mesenchymal differentiation in vivo. Thus, negative regulation of Sox10 by TGFb signaling promotes the generation of mesenchymal progenitors from NCSCs. Our study might lay the grounds for future applications demanding defined populations of MSCs for regenerative medicine. STEM CELLS 2011;29:689-699 Disclosure of potential conflicts of interest is found at the end of this article.

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Expression of chondrogenic potential of mouse trunk neural crest cells by FGF2 treatment

Cited by 42 publications

References 23 publications

Sonic Hedgehog promotes the development of multipotent neural crest progenitors endowed with both mesenchymal and neural potentials

Sonic Hedgehog promotes the development of multipotent neural crest progenitors endowed with both mesenchymal and neural potentials

The emergence of ectomesenchyme

Transforming Growth Factor β-Mediated Sox10 Suppression Controls Mesenchymal Progenitor Generation in Neural Crest Stem Cells

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