Chemosensitivity testing with highly purified fresh human tumour cells with the MTT colorimetric assay

Yamaue, Hiroki; Tanimura, Hiroshi; Tsunoda, Takuya; Tani, Masaji; Itō, Makoto; Noguchi, Kohei; Tamai, Mikiko; Hotta, Tsukasa; Arii, Kazuo

doi:10.1016/0277-5379(91)90093-s

Cited by 46 publications

(29 citation statements)

References 13 publications

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“…41,42) Thus, unlike the subcutaneous tumor model, the selective transduction of exogenous genes to tumor cells by an intravenous administration of adenoviral vectors cannot be expected in a hepatic metastasis model. However, tumor cells are usually more susceptible to chemotherapeutic drugs than are normal cells, 43) and the therapeutic strategy for hepatic metastasis is focused on the hepatocytes expressing the CD gene or IL-2 gene. We speculate that the mechanism of tumor rejection in a hepatic metastasis model may be conversion of the 5-FC to 5-fluorouracil (5-FU) and the secretion of sufficient amounts of 5-FU to promote toxicity, and sufficient IL-2 to induce antitumor immunity against nearby tumor cells without concomitant hepatocyte toxicity.…”

Section: Discussionmentioning

confidence: 99%

Dose of Adenoviral Vectors Expressing Interleukin‐2 Plays an Important Role in Combined Gene Therapy with Cytosine Deaminase/5–Fluorocytosine: Preclinical Consideration

Nakamori

Itō

Ueda

et al. 2002

Japanese Journal of Cancer Research

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Section: Discussionmentioning

confidence: 99%

Dose of Adenoviral Vectors Expressing Interleukin‐2 Plays an Important Role in Combined Gene Therapy with Cytosine Deaminase/5–Fluorocytosine: Preclinical Consideration

Nakamori

Itō

Ueda

et al. 2002

Japanese Journal of Cancer Research

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“…The SDI test is simple, fast, and economical, but tumor cell sensitivity may not be correctly evaluated because the results can be influenced by fibroblast contamination [15,16] . Yamaue et al [7] practically eliminated this drawback by purifying tumor cells using frequently repeated density gradient centrifugation. Graham et al [17] demonstrated the importance of using a three-dimensional culture model in which spheroids of tumor cells show resistance to anticancer drugs and radiation more clearly than in monolayer cell cultures.…”

Section: Discussionmentioning

confidence: 99%

“…The procedure described by Yamaue et al [7] was employed for purification of the cancer cells obtained from the ascitic fluid specimens. The ascitic fluid was immediately centrifuged at 400 g for 5 min and then suspended in complete medium.…”

Section: Purification Of Cancer Cells Obtained From Ascitesmentioning

confidence: 99%

A New Chemosensitivity Assay for Ascites Tumor Cells Using a Thermoreversible Gelation Polymer as a Culture Medium and the Observed Clinical Responses

et al. 2007

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Background/Aim: Ascites tumor cells from patients with peritonitis carcinomatosa were tested for cis-diamminedichloroplatinum (CDDP) sensitivity. The patients were divided into CDDP-sensitive and resistant groups. Survival and time to progression (TTP) rates were compared. Materials and Methods: 18 peritonitis carcinomatosa patients with class V ascites based on cytologic diagnosis were enrolled in this study. Chemosensitivity testing of the ascites tumor cells was done to determine their sensitivity to CDDP using a three-dimensional culture matrix of thermoreversible gelation polymer (TGP). CDDP at a dose calculated to achieve ascitic fluid drug levels equivalent to the IC₅₀ was given intraperitoneally to 12 CDDP-sensitive patients and 6 CDDP-resistant patients. Results: Both the median survival time and the median TTP were significantly longer in CDDP-sensitive patients than in CDDP-resistant patients (survival time 105 vs. 13 days, p = 0.019; TTP 90 vs. 5 days, p = 0.029). Conclusion: The results indicate the potential feasibility of controlling ascites in cancer patients in whom a maximal dose effect can be achieved with a minimal dose of chemotherapy.

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“…However, the above TCAs are still limitations on drug sensitivity and specificity (Yamaue et al, 1991;Huh et al, 2009). Recent years, the analysis of endogenous ATP was reported to be the most predictive of toxicity testing methods (Ekwall & Sussman, 2000).…”

Section: Introductionmentioning

confidence: 99%

Clinical Application of the Adenosine Triphosphate-based Response Assay in Intravesical Chemotherapy for Superficial Bladder Cancer

Pu²,

Zheng³

2012

Asian Pacific Journal of Cancer Prevention

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Objective: To investigate correlations between adenosine triphosphate chemotherapy response assay (ATP-CRA) and clinical outcomes after ATP-CRA-based chemotherapy for drug selection in patients receiving intravesical chemotherapy to prevent recurrence of superficial bladder cancer after surgery. Methods: The chemosensitivities of 12 anticancer drugs were evaluated, including 5-Fu ADM, and EPI, using ATP-CRA and primary tumor cell culture in 54 patients. In addition, a further 58 patients were treated according to clinical experience. Differences in post-chemotherapeutical effects between drug sensitivity assay and experience groups were compared. Results: The evaluable rate of the test was 96.3%, the clinical effective rate was 80.8%, the sensitivity rate was 97.6% (41/42), the specificity was 20%, the total predicting accuracy was 74.3%, the positive predictive value was 83.7% (41/49), the negative predictive value was 66.7% (2/3); in the drug sensitivity test group, the clinical effective rate was 80.8%, the experience group response rate was 63.8%, with a significant difference in clinical effects between the ATP-based sensitivity and experience groups (χ 2 =7.0153, P<0.01). Conclusion: ATP-CRA is a stable, accurate and potentially practical chemosensitivity test providing a predictor of chemotherapeutic response in patients with superficial bladder cancer.

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Chemosensitivity testing with highly purified fresh human tumour cells with the MTT colorimetric assay

Cited by 46 publications

References 13 publications

Dose of Adenoviral Vectors Expressing Interleukin‐2 Plays an Important Role in Combined Gene Therapy with Cytosine Deaminase/5–Fluorocytosine: Preclinical Consideration

Dose of Adenoviral Vectors Expressing Interleukin‐2 Plays an Important Role in Combined Gene Therapy with Cytosine Deaminase/5–Fluorocytosine: Preclinical Consideration

A New Chemosensitivity Assay for Ascites Tumor Cells Using a Thermoreversible Gelation Polymer as a Culture Medium and the Observed Clinical Responses

Clinical Application of the Adenosine Triphosphate-based Response Assay in Intravesical Chemotherapy for Superficial Bladder Cancer

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