Chemoprotective effect of vitexin against cisplatin-induced biochemical, spermatological, steroidogenic, hormonal, apoptotic and histopathological damages in the testes of Sprague-Dawley rats

Ijaz, Muhammad Umar; Tahir, Arfa; Ahmed, Hussain; Ashraf, Asma; Ahmedah, Hanadi Talal; Muntean, Liviu; Moga, Marius; Irimie, Marius

doi:10.1016/j.jsps.2022.03.001

Cited by 18 publications

(8 citation statements)

References 63 publications

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“…Changes in these expressions were appraised by 2 -ΔΔCT using β-actin as an internal control. Table 1 displays the primer sequences of the target genes, as reported previously ( 25 , 26 ).…”

Section: Methodsmentioning

confidence: 99%

Didymin protects against polystyrene nanoplastic-induced hepatic damage in male albino rats by modulation of Nrf-2/Keap-1 pathway

Ijaz,

Nadeem,

Hamza

et al. 2024

Braz J Med Biol Res

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Polystyrene nanoplastics (PS-NPs) are ubiquitous environmental pollutants that can cause oxidative stress in various organs, including the liver. Didymin is a dietary flavanone that displays multiple pharmacological activities. Therefore, the present study evaluated the palliative role of didymin against PS-NPs-induced hepatic damage in rats. Albino rats (n=48) were randomly distributed into 4 groups: control, PS-NPs treated group, PS-NPs + didymin co-administered group, and didymin supplemented group. After 30 days, PS-NPs intoxication lowered the expression of Nrf-2 and anti-oxidant genes [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GSR), glutathione-S-transferase (GST), and heme oxygenase-1 (HO-1 )], whereas the expression of KEAP1 kelch like ECH associated protein 1 (Keap-1) was increased. PS-NPs exposure also reduced the activities of anti-oxidants enzymes (CAT, SOD, GPx, GSR, GST, GSH, and OH-1), while malondialdehyde (MDA) and reactive oxygen species (ROS) levels were increased. The levels of alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were increased in PS-NPs-exposed rats. Moreover, inflammatory indices [interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), nuclear factor-kappa B (NF-κB), and cyclooxygenase-2 (COX-2)] were increased in PS-NPs-exposed rats. Furthermore, PS-NPs intoxication increased the expressions of apoptotic markers including Bax and Caspase-3 , as well as reducing Bcl-2 expression. The histopathological analysis showed significant damage in PS-NPs-treated rats. However, didymin supplementation ameliorated all the PS-NPs-induced damage in the liver of rats. Therefore, it was concluded that didymin can act as a remedy against PS-NPs-induced liver toxicity due to its anti-apoptotic, anti-oxidant, and anti-inflammatory activities.

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Section: Methodsmentioning

confidence: 99%

Didymin protects against polystyrene nanoplastic-induced hepatic damage in male albino rats by modulation of Nrf-2/Keap-1 pathway

Ijaz,

Nadeem,

Hamza

et al. 2024

Braz J Med Biol Res

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“…Changes in these expressions were determined by

{2}^{ \mbox{-} \mathrm{\Delta \Delta }{C}_{{\rm{T}}}}

by using β‐actin as a housekeeping gene 38 . The primers of target genes are presented in Table 1, as reported previously 39,40 …”

Section: Methodsmentioning

confidence: 99%

Attenuative effects of tamarixetin against polystyrene microplastics‐induced hepatotoxicity in rats by regulation of Nrf‐2/Keap‐1 pathway

Ijaz,

Khalil,

Hamza

et al. 2023

Cell Biochemistry & Function

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Polystyrene microplastics (PS‐MPs) are environmental contaminants due to their potential to induce damages in multiple organs specifically liver. Tamarixetin (TMT) is a naturally occurring flavonoid present in Tamarix ramosissima plant that exhibits multiple pharmacological properties. Therefore, the present research was designed to evaluate the palliative role of TMT against PS‐MPs instigated liver dysfunction in rats. The exposure to PS‐MPs reduced the expressions of nuclear factor erythroid 2‐related factor 2 and antioxidant genes, while increasing the expression of Kelch‐like ECH‐associated protein 1. PS‐MPs exposed rats exhibited considerably (p < .05) higher alkaline phosphatase (ALP), aspartate aminotransferase (AST) as well as alanine aminotransferase (ALT) contents. Additionally, PS‐MPs treatment resulted in a notable decrease in anti‐oxidants activity, that is, glutathione S‐transferase (GST), superoxide dismutase (SOD), heme oxygenase‐1 (HO‐1), glutathione reductase (GSR), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) content, whereas upregulating reactive oxygen species (ROS) and malondialdehyde (MDA) contents. Moreover, PS‐MPs intoxication noticeably increased (p < .05) the inflammatory indices (interleukin‐1ß [IL‐1ß], nuclear factor kappa B [NF‐κB], interleukin‐6 [IL‐6], tumor necrosis factor‐α [TNF‐α] levels, and cyclooxygenase‐2 [COX‐2] activity). Besides, Caspase‐3 and Bax expressions were upregulated and Bcl‐2 expression was decreased after PS‐MPs exposure. Additionally, the histomorphological examination revealed notable hepatic damage in PS‐MPs treated group. However, TMT treatment substantially (p < .05) recovered all the PS‐MPs‐induced damages and histopathological changes. Taken together, it can be deduced that TMT might be used as a pharmacological agent to ameliorate hepatic damage.

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“…The negative effects of cancer treatment on fertility have directed researchers towards the discovery of agents targeting the preservation of fertility (Sabanegh & Ragheb, 2009). The presence of cisplatin-induced testicular damage is confirmed by the studies in the literature (Elsayed et al, 2022;Ijaz et al, 2022;Sawaya et al, 2022;Kuribayashi et al, 2023). However, to our knowledge, there are no studies in the literature that have investigated the use of white tea or infliximab for the reversal of cisplatin-induced testicular damage.…”

Section: Introductionmentioning

confidence: 96%

“…Although the specific molecular mechanism underlying the toxicity of cisplatin in testicular tissue is not clearly known, the mechanism that is most strongly emphasized involves oxidative stress, inflammation, and apoptosis (Jahan et al, 2018;Bostancıeri et al, 2022). Therefore, a variety of antioxidant agents have been studied in the literature in order to reverse cisplatin-induced testicular damage (Aly & Eid, 2020;Wang et al, 2020;Altındag˘ & Meydan, 2021;Elsayed et al, 2022;Ijaz et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

The Effects of White Tea (Camellia Sinensis) and Infliximab Against Cisplatin- Induced Testicular Damage via Oxidative Stress and Apoptosis

Cakıroglu,

Mercantepe,

Tumkaya

et al. 2023

Int. J. Morphol.

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Chemoprotective effect of vitexin against cisplatin-induced biochemical, spermatological, steroidogenic, hormonal, apoptotic and histopathological damages in the testes of Sprague-Dawley rats

Cited by 18 publications

References 63 publications

Didymin protects against polystyrene nanoplastic-induced hepatic damage in male albino rats by modulation of Nrf-2/Keap-1 pathway

Didymin protects against polystyrene nanoplastic-induced hepatic damage in male albino rats by modulation of Nrf-2/Keap-1 pathway

Attenuative effects of tamarixetin against polystyrene microplastics‐induced hepatotoxicity in rats by regulation of Nrf‐2/Keap‐1 pathway

The Effects of White Tea (Camellia Sinensis) and Infliximab Against Cisplatin- Induced Testicular Damage via Oxidative Stress and Apoptosis

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