Didymin protects against polystyrene nanoplastic-induced hepatic damage in male albino rats by modulation of Nrf-2/Keap-1 pathway

Ijaz, M.U.; Nadeem, N.; Hamza, A.; Almutairi, M.H.; Atique, U.

doi:10.1590/1414-431x2023e13173

Cited by 1 publication

(1 citation statement)

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“…However, here we showed that the tested flavonoid aglycones possess strong antiplatelet effects; thus, elevated risks of bleeding should not be excluded in these patients applying flavonoid aglycones as comedication. Flavonoids also appear as strong anti-apoptotic agents in non-cancer cells [103,104]; consequently, it is essential to investigate the anti-apoptotic effect of the tested compounds in platelets.…”

Section: Discussionmentioning

confidence: 99%

Antiplatelet Effects of Flavonoid Aglycones Are Mediated by Activation of Cyclic Nucleotide-Dependent Protein Kinases

Balykina,

Naida,

Kirkgöz

et al. 2024

IJMS

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Flavonoid aglycones are secondary plant metabolites that exhibit a broad spectrum of pharmacological activities, including anti-inflammatory, antioxidant, anticancer, and antiplatelet effects. However, the precise molecular mechanisms underlying their inhibitory effect on platelet activation remain poorly understood. In this study, we applied flow cytometry to analyze the effects of six flavonoid aglycones (luteolin, myricetin, quercetin, eriodictyol, kaempferol, and apigenin) on platelet activation, phosphatidylserine externalization, formation of reactive oxygen species, and intracellular esterase activity. We found that these compounds significantly inhibit thrombin-induced platelet activation and decrease formation of reactive oxygen species in activated platelets. The tested aglycones did not affect platelet viability, apoptosis induction, or procoagulant platelet formation. Notably, luteolin, myricetin, quercetin, and apigenin increased thrombin-induced thromboxane synthase activity, which was analyzed by a spectrofluorimetric method. Our results obtained from Western blot analysis and liquid chromatography–tandem mass spectrometry demonstrated that the antiplatelet properties of the studied phytochemicals are mediated by activation of cyclic nucleotide-dependent signaling pathways. Specifically, we established by using Förster resonance energy transfer that the molecular mechanisms are, at least partly, associated with the inhibition of phosphodiesterases 2 and/or 5. These findings underscore the therapeutic potential of flavonoid aglycones for clinical application as antiplatelet agents.

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Section: Discussionmentioning

confidence: 99%