Chemoprotective Effect of Taurine on Potassium Bromate-Induced DNA Damage, DNA-Protein Cross-Linking and Oxidative Stress in Rat Intestine

Abstract

Potassium bromate (KBrO3) is widely used as a food additive and is a major water disinfection by-product. It induces multiple organ toxicity in humans and experimental animals and is a probable human carcinogen. The present study reports the protective effect of dietary antioxidant taurine on KBrO3-induced damage to the rat intestine. Animals were randomly divided into four groups: control, KBrO3 alone, taurine alone and taurine+ KBrO3. Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered. There was increase in DNA damage and DNA-protein cross-linking. Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect. Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group. These results show that taurine ameliorates bromate induced tissue toxicity and oxidative damage by improving the antioxidant defence, tissue integrity and energy metabolism. Taurine can, therefore, be potentially used as a therapeutic/protective agent against toxicity of KBrO3 and related compounds.

Altered hepatic mRNA expression of immune response-associated DNA damage in mice liver induced by potassium bromate: Protective role of vanillin

“…However, this reduction is sometimes incomplete, and residual KBrO 3 remains in the bread, making it a source of potential carcinogen to humans. KBrO 3 is also used in treating barley in beer making, in cheese production, and is commonly added to fish paste products in Japan (Ahmad et al, ).…”

Roles of Bacillus subtilis RecA, Nucleotide Excision Repair, and Translesion Synthesis Polymerases in Counteracting Cr(VI)-Promoted DNA Damage

“…To investigate this notion, we determined the formation of DPCs in B. subtilis cells deficient in RecA, UvrA, or both proteins, which were grown in the presence or absence of hexavalent chromium. It must be emphasized that the protocol employed to detect DPCs in our study has been widely employed and validated not only in Bacillus subtilis but also in other biological systems (48)(49)(50)(51)(52). As shown in Fig.…”

Anti-mutagenic Action of Ruta chalepensis against Rat Sperm Cell Abnormalities-induced by Potassium Bromate

“…AURKC is a member of serine/threonine protein kinases and is a component of the chromosomal passenger complex (COC) which is an essential regulator of mitotic events, including chromosome segregation and cytokinesis. [40] Defects of the AURKC protein lead to disruptions in meiosis and to chromosomally unbalanced gametes [36] resulting in polyploidy sperm cells with enlarged and irregular shaped heads and multiple flagella. SPATA16 is localized at the Golgi apparatus and plays an essential role in the acrosome formation during spermatogenesis.…”

“…Mutations in the DNAH1 gene show general axonemal disorganization including mislocalization of the microtubule doublets and loss of the inner arm dyneins. In addition, disorganization of the fibrous sheath, the outer dense fibres and the mitochondrial sheath leading to multiple morphological irregularities of the tail such as absent, bent, coiled and tail of irregular width [39,40].…”