Altered hepatic mRNA expression of immune response-associated DNA damage in mice liver induced by potassium bromate: Protective role of vanillin

Abstract

Chronic exposure to potassium bromate (KBrO ), a toxic halogen existing widely in the environment, environment through contaminated drinking water, has become a global problem of public health. The present study investigates the protective role of vanillin against KBrO induced oxidative stress, distruption in inflammatory cytokines expression, DNA damage, and histopathological changes. Adult mice were exposed orally to KBrO (2g/L of drinking water) for 2 weeks The co-administration of vanillin to the KBrO -treated mice significantly prevented the plasma transaminases increase in. Furthermore, it inhibited hepatic lipid peroxidation (malondialdehyde), advanced oxidation protein product (AOPP) and protein carbonyl (PCO) formation and attenuated the KBrO -mediated depletion of enzymatic and non enzymatic antioxidants catalase, superoxide dismutase, and glutathione peroxidase activities and glutathione level in the liver. In addition, vanillin markedly attenuated the expression levels of proinflammatory cytokines, including tumor necrosis factor-α, interleukin-1β, interleukin-6, and COX2 and prevented KBrO -induced hepatic cell alteration and necrosis, as indicated by histopathological data. DNA damage, as assessed by the alkaline comet assay, was also found to be low in the co-treated group. Thus, these findings show that vanillin acts as potent chemopreventive agent against KBrO -mediated liver oxidative stress and genotoxicity through its antioxidant properties. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1796-1807, 2016.

Beneficial role of vanillin, a polyphenolic flavoring agent, on maneb-induced oxidative stress, DNA damage, and liver histological changes in Swiss albino mice

“…Coadministration of vanillin, a polyphenolic flavoring agent, to maneb-treated mice restored MDA, H 2 O 2 , and AOPP levels, without reaching control values. Similar findings have been found by Ben Saad et al, 46 where vanillin attenuates the increase in lipid peroxidation, AOPP, and protein carbonyl formation in the liver of mice treated with potassium bromate. Also, Kadeche et al 10 have reported that vanillin exhibits hepatoprotective effect against metribyzin pesticide toxicity in male Wistar rats.…”

Overview of the Role of Vanillin on Redox Status and Cancer Development

“…The protective effect of vanillin against KBrO 3 induced liver, bone, and blood disorders has also been investigated [58, 59]. Coadministration of vanillin to KBrO 3 -treated mice significantly prevented DNA damage, hepatic cell alteration, and plasma transaminases increases, inhibited hepatic lipid peroxidation, and attenuated depletion of enzymatic and nonenzymatic antioxidants and expression levels of proinflammatory cytokines, including tumor necrosis factor- α , interleukin-1 β , interleukin-6, and COX2.…”

Extensive Summary of the Important Roles of Indole Propionic Acid, a Gut Microbial Metabolite in Host Health and Disease

“…For example, KBrO 3 is a carcinogen that can cause oxidative stress [ 118 ]. Although it was banned from the food processing industry, the presence of KBrO 3 in the environment has still harmed human health [ 119 ], largely via targeting the kidney and thyroid to induce tumorigenesis [ 120 ]. Classic antioxidant enzymes, such as superoxide dismutase (SOD) and catalase, have little protective effect on KBrO 3 -induced oxidative damage.…”