Chemopreventive properties of raisins originating from Greece in colon cancer cells

Abstract

Colorectal cancer is one of the major causes of cancer-related mortality in humans in both developed and developing countries. Dietary patterns influence the risk of colon cancer development, while plant-derived foods have gained great interest, due to the high content of antioxidants. Corinthian raisins (Currants, CR) and Sultanas (S) (Vitis vinifera L., Vitaceae) are dried vine fruits produced in Greece with many culinary uses in both the Mediterranean and the Western nutrition. In the present study, we investigated the effects of CR and S on human colon cancer cells. Methanol extracts of CR and S were used at different concentrations. The total polyphenol content and anti-radical activity were measured by Folin-Ciocalteu and DPPH, respectively. Antioxidant, anti-inflammatory and anti-proliferative effects on HT29 cell culture were evaluated. All extracts exhibited DPPH˙ scavenging activity in a dose-dependent manner. Both products suppressed cell proliferation, while the levels of glutathione and cyclooxygenase 2 were significantly decreased. A significant reduction in IL-8 levels and NF-kappaB p65 activation was also observed. Both antioxidant and anti-inflammatory effects were dependent on the duration of exposure. Results indicate that the methanol extracts of CR and S exhibit anti-radical activity in vitro, as well as cancer preventive efficacy on colon cancer cells, with S having slightly higher activity. The beneficial properties of these unique dried grapes are attributed to their high content of phenolic compounds.

Modulators affecting the immune dialogue between human immune and colon cancer cells

“…Moreover, studies have shown that it may act as an inhibitor of cancer development [77][78][79] . The mechanisms explaining resveratrol's anti-inflammatory properties have been reviewed by de la Lastra et al [80] .…”

Antiproliferative activities of several plant extracts from Turkey on rat brain tumor and human cervix carcinoma cell lines

“…On the other hand, V. vinifera extract effect decreases in lower concentrations (5-50 μg/mL). Different parts of V. vinifera have been widely investigated against different types of cancer (Kaliora et al 2008;Amico et al 2009;Lazze et al 2009;Sung & Lee 2010;Nechita et al 2012;Apostolou et al 2013;Esfahanian et al 2013;Espino et al 2013;Kountouri et al 2013;Giovannelli et al 2014;Liang et al 2014;Sahpazidou et al 2014) and it has been reported that V. vinifera has an antiproliferative effect against many cancer cell lines. Naturally occurring bioactive component of V. vinifera that is responsible from the cardioprotective and cancer chemopreventive activities (Jang et al 1997) is identified as resveratrol (3,4 ,5-trihydroxystilbene) (Frankel et al 1993).…”

Greek raisins: A traditional nutritious delicacy

“…Raisins are also rich in phytochemicals, which may convey protection against colorectal [16] and gastric [18] cancer and may also play a role in the prevention of cardiovascular disease through a triglyceride-and cholesterollowering effect [19]. They have also been reported to provide protection against diabetes by lowering the postprandial insulin response and modulating sugar absorption [20].…”

“…As raisins are a rich source of fiber, a single serving of raisins provides a significant amount of fiber in a daily diet and can have beneficial effects on colon health [16]. Moreover, evidence suggests that dietary fiber may act synergistically with tartaric acid in raisins to stimulate the growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial to health [17].…”