Chemopreventive potential of alpha lipoic acid in the treatment of colon and cervix cancer cell lines

Damnjanović, Ivana; Kocić, Gordana; Najman, Stevo; Stojanović, Sanja; Stojanović, Dušica; Veljković, Andrej; Conic, Irena; Langerholc, Tomaž; Pesic, Srdjan

doi:10.4149/bll_2014_118

Cited by 16 publications

(18 citation statements)

References 30 publications

(27 reference statements)

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“…LA alone or combined with a chemotherapy drug can inhibit colon cancer and cervical cancer cells proliferation. LA may affect tumor through regulating NF-κB (Damnjanovic et al, 2014;Li et al, 2015) (Damnjanovic et al, 2014;Li et al, 2015). NF-κB belongs to the set of nucleoprotein factors that has a multidirectional transcription-regulating effect.…”

Section: Discussionmentioning

confidence: 99%

“…Based on previous studies (Damnjanovic et al, 2014;Lim et al, 2015) (Damnjanovic et al, 2014;Lim et al, 2015), MCF-7 cells were randomly divided into four groups, the control group; LA-alone group, treated with 50 μM LA; paclitaxel-alone group, treated with 20 μg/mL paclitaxel; and combined group, which received 50 μM LA and 20 μg/mL paclitaxel.…”

Section: Cell Culture and Groupingmentioning

confidence: 99%

“…LA can play a protective role in diabetes and diabetic complications, as well as being used following ischemia-reperfusion injury in critical organs such as heart, brain, and kidney (Bingham et al, 2014) (Bingham et al, 2014). Recent research confirmed that LA can inhibit cancer cell proliferation in cervical cancer and colon cancer (Damnjanovic et al, 2014) (Damnjanovic et al, 2014). This study intends to explore the possible mechanisms of LA alone and with paclitaxel application in breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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Effect of lipoic acid combined with paclitaxel on breast cancer cells

Ren

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Breast cancer is the most common gynecologic tumor globally that threatens women's health. Lipoic acid is a type of antioxidant that can alleviate oxidative stress damage. Studies showed that lipoic acid could inhibit the proliferation of tumor cells in cervical cancer and colon cancer. This paper intends to explore the combined effect of lipoic acid and paclitaxel on breast cancer cells. Breast cancer MCF-7 cells were divided into four groups: control group, lipoic acid group, paclitaxel group, and a combination group. MTT was applied to detect the drugs' influence on breast cancer cell proliferation. A colony formation test was used to determine the effects on breast cancer cell clone formation rate. Western blot was performed to detect the effects on nuclear factor (NF)-κB. Lipoic acid alone can inhibit tumor cell proliferation and clone formation with time dependence. Compared with the control, paclitaxel alone can significantly suppress tumor cell proliferation and clone formation (P < 0.05). Lipoic acid and paclitaxel in combination obviously strengthened their individual inhibitory effects on tumor cells (P < 0.05). Compared with 17935 Lipoic acid combined with paclitaxel on breast cancer ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17934-17940 (2015) the paclitaxel alone group, the combination group exhibited more remarkable inhibitory effect (P < 0.05). Lipoic acid alone or combined with paclitaxel can inhibit NF-κB expression and inhibit breast cancer cell proliferation.

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Section: Discussionmentioning

confidence: 99%

Section: Cell Culture and Groupingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of lipoic acid combined with paclitaxel on breast cancer cells

Ren

et al. 2015

Genet. Mol. Res.

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“…Cell growth was examined by performing MTT test according to the protocol by Damnjanovic et al (20). Results are presented as: the mean value of absorbance ± standard deviation from four to eight replications for the assayed compounds as well as control.…”

Section: Mtt Testmentioning

confidence: 99%

Molecular Mechanisms of Potential Synergi Stic Effect of Ketoprofen and Melox Icam With Convention Al Cytostatics in Human Cervix Cancer Cell Line

Damnjanović¹,

Kocić²,

Najman³

et al. 2018

AMM

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Cyclooxygenases clearly appear to be implicated in carcinogenesis. It has been reported that COX-2 is active throughout the entire process of cancer development and progression. Various molecular mechanisms may be responsible for this. Epidemiological and experimental studies have revealed that nonselective non-steroidal anti-inflammatory drugs (NSAIDs) and selective COX-2 inhibitors can reduce the risk of cancer. Inhibition of COX provides a plausible explanation of the data on NSAIDs and cancer. However, the molecular pathways of this effect are still unclear, more complex and likely involve multiple COX-2dependent and independent mechanisms, where pro-and anti-apoptotic Bcl-2 family members may take part. We examined the effects of ketoprofen (KT), as nonselective COX-1/2, and meloxicam (MK), as selective COX-2 inhibitor, alone and combined with 5-fluorouarcil (FU) and cisplatin (CP), on the proliferation by MTT test and Bcl-2/Bax expression in HeLa cells (human cervical carcinoma cells). MC alone or combined with conventional anticancer drugs, FU and CP, showed better cytotoxic and antiproliferative effect than KT. The levels of Bcl-2 were decreased while the levels of Bax were increased dose-dependently by KT and MC. A significant increase in the expression of Bax protein in HeLa cells was more pronounced for MC. The synergy observed in the effects of ketoprofen and meloxicam with cisplatin and 5fluorouracil on the cervical cancer cell line was generated by an enhancement of apoptosis. Therefore, ketoprofen and meloxicam may represent therapeutic candidates to improve access of cervical cancer chemoprevention and chemotherapy.

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“…1 This disease is produced due to abnormal growth of cells that can invade or spread in other parts of the body. 2 Statistics show that in 2014 about 71,830 men and 65,000 women were diagnosed with colon cancer in the United States 3 and its 5-year survival rate in the United States is about 65%.…”

Section: Introductionmentioning

confidence: 99%

MiR-339 and especially miR-766 reactivate the expression of tumor suppressor genes in colorectal cancer cell lines through DNA methyltransferase 3B gene inhibition

Afgar

Fard-Esfahani

Mehrtash

et al. 2016

Cancer Biology & Therapy

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It is observed that upregulation of DNMT3B enzyme in some cancers, including colon cancer, could lead to silencing of tumor suppressor genes. MiR-339 and miR-766 have been predicted to target 3 0 UTR of DNMT3B gene. Luciferase reporter assay validated that individual and co-transfection of miR-766 and miR-339 into the HEK293T cell reduced luciferase activity to 26% § 0.41%, 43% § 0.42 and 64% § 0.52%, respectively, compared to the control (P < 0.05). Furthermore, transduction of miR-339 and miR-766 expressing viruses into colon cancer cell lines (SW480 and HCT116) decreased DNMT3B expression (1.5, 3-fold) and (3, 4-fold), respectively. In addition, DNA methylation of some tumor suppressor genes decreased. Expression of these genes such as SFRP1 (2 and 1.6-fold), SFRP2 (0.07 and 4-fold), WIF1 (0.05 and 4-fold), and DKK2 (2 and 4-fold) increased in SW-339 and SW-766 cell lines; besides, expression increments for these genes in HCT-339 and HCT-766 cell lines were (2.8, 4-fold), (0.005, 1.5-fold), (1.7 and 3-fold) and (0.04, 1.7-fold), respectively. Also, while in SW-766, cell proliferation reduced to 2.8% and 21.7% after 24 and 48 hours, respectively, SW-339 showed no reduced proliferation. Meanwhile, HCT-766 and HCT-339 showed (3.5%, 12.8%) and (18.8%, 33.9%) reduced proliferation after 24 and 48 hours, respectively. Finally, targeting DNMT3B by these miRs, decreased methylation of tumor suppressor genes such as SFRP1, SFRP2, WIF1 and DKK2 in the mentioned cell lines, and returned the expression of these tumor suppressor genes which can contribute to lethal effect on colon cancer cells and reducing tumorigenicity of these cells.

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Chemopreventive potential of alpha lipoic acid in the treatment of colon and cervix cancer cell lines

Cited by 16 publications

References 30 publications

Effect of lipoic acid combined with paclitaxel on breast cancer cells

Effect of lipoic acid combined with paclitaxel on breast cancer cells

Molecular Mechanisms of Potential Synergi Stic Effect of Ketoprofen and Melox Icam With Convention Al Cytostatics in Human Cervix Cancer Cell Line

MiR-339 and especially miR-766 reactivate the expression of tumor suppressor genes in colorectal cancer cell lines through DNA methyltransferase 3B gene inhibition

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