Chemomorphic Analysis of Malathion in Skin Layers of the Rat: Implications for the Use of Dermatopharmacokinetic Tape Stripping in Exposure Assessment to Pesticides

Dary, Curtis C.; Blancato, Jerry N.; Saleh, Mahmoud A.

doi:10.1006/rtph.2001.1462

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…18 Recent compilations 19 describe biomedical applications, in general, and several reports specifically describe IR spectroscopic studies on human skin. The skinfocused reports 20 examine the structure of skin, [21][22][23][24] diseases, [25][26][27][28][29] dynamics of diffusion [30][31][32][33][34][35][36][37] or use it as a model system for studies. 38 There are no reports, to our knowledge, on analyzing engineered skin with IR spectroscopic imaging.…”

Section: Introductionmentioning

confidence: 99%

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Kong

Reddy

Bhargava

2010

Analyst

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Engineered tissues can provide models for imaging and disease progression and the use of such models is becoming increasingly prevalent. While structural characterization of these systems is documented, a combination of biochemical and structural knowledge is often helpful. Here, we apply Fourier transform infrared (FT-IR) spectroscopic imaging to examine an engineered tissue model of melanoma. We first characterize the biochemical properties and spectral changes in different layers of growing skin. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. In particular, chemical changes associated with tumor-stromal interactions are observed during the course of tumor growth and appear to influence a 50-100 μm region. The development of this analytical approach combining engineered tissue with spectroscopy, imaging and computation will allow for quality control and standardization in tissue engineering and novel scientific insight in cancer progression.

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Section: Introductionmentioning

confidence: 99%

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Kong

Reddy

Bhargava

2010

Analyst

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“…From in vivo studies in rats (Rougier et al ., ) and humans (Dupuis et al ., ) in which numerous test molecules with different physiochemical properties were applied to skin, Rougier A. et al . demonstrated a linear relationship between two entities as (1) amount of test substance present in SC reservoir 30 min post‐application as measured by liquid scintillation counting after tape‐stripping the treated area, and (2) total amount of test molecule absorbed over 96 h. From another in vivo study in rats for risk assessment of toxic substances, the measurement of drug penetration into SC with respect to time was related linearly to drug distribution in underlying tissues (Dary et al ., ). When evaluating SC reservoir capacity of intact porcine tissue in vitro , Jacobi et al .…”

Section: Introductionmentioning

confidence: 97%

Stratum corneum reservoir as a predictive method for in vitro percutaneous absorption

Hafeez

Chiang

Hui

et al. 2015

J of Applied Toxicology

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Interaction between drug and proteins and lipids in stratum corneum (SC) is an important pharmacokinetic parameter in early steps of absorption. Previous in vivo studies showed that the total amount of compound, regardless of properties, penetrating over a 96 h period could be predicted by the amount present in SC 30 min after application by a linear relationship. Validating this linear relationship through in vitro study would facilitate testing of transdermal drug delivery platforms. We aimed to determine in vitro penetration behavior across SC of humans by determining the relationship between quantity present in SC reservoir 30 min after application with 24 h skin absorption and penetration. In this study, use of the SC reservoir effect to predict absorption and penetration of topical compounds is reaffirmed with in vitro models involving human skin. These results indicate the amount in short-term (30 min) SC reservoir predict long-term (24 h) skin absorption and penetration, as characterized by statistically significant linear relationships determined via regression. This may be explained by the fact that SC is a rate-limiting barrier to percutaneous drug transport. After molecules diffuse through SC barrier, passage into deeper dermal layers and systemic uptake occur relatively quickly. These results enable one to measure quantity in SC reservoir shortly after topical application as a proxy for absorption and penetration over longer periods. With respect to drug development and risk assessment of toxic substances, this may simplify assays attempting to quantitate penetration capacity. Further investigation with a larger range of compounds is needed to clarify the observations recorded here. Copyright © 2015 John Wiley & Sons, Ltd.

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“…Tape-stripping, the sequential removal of SC layers using adhesive tapes, has been widely used in numerous pharmacological and dermatological applications. [7][8][9] The tapes used for stripping-containing ripped off SC material together with any exogenous chemical that may have penetrated into itcan be extracted and the chemical quantified by highperformance liquid chromatography ͑HPLC͒, gas chroatography ͑GC͒, scintillation counting, ultraviolet-visible ͑UV-vis͒, or infrared ͑IR͒ spectroscopy ͑for a recent review, see Ref.…”

Section: Introductionmentioning

confidence: 99%

Application of attenuated total reflection Fourier transform infrared imaging and tape-stripping to investigate the three-dimensional distribution of exogenous chemicals and the molecular organization in Stratum corneum

2008

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Attenuated total reflection Fourier transform infrared spectroscopic imaging combined with tape-stripping is an advantageous approach to map the depth penetration and lateral distribution of topically applied chemicals in Stratum corneum (SC) and the conformational order of SC lipids. Tape-stripping progressively removes layers of SC, and chemical imaging provides spatially resolved information on the chemical composition of both the newly exposed SC surface and of the tapes used for stripping. The procedure is rapid, minimally invasive, and does not necessitate cross-sectioning of the skin. This approach offers a simple and direct way to determine the distribution of exogenous volatile and non-volatile chemicals in SC as a function of the chemical composition of the formulation and time, and the conformational order of SC lipids in native and topically treated skin. The procedure described here is well suited to address questions of relevance for the areas of drug delivery, dermatology, and skin care.

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Chemomorphic Analysis of Malathion in Skin Layers of the Rat: Implications for the Use of Dermatopharmacokinetic Tape Stripping in Exposure Assessment to Pesticides

Cited by 7 publications

References 34 publications

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Stratum corneum reservoir as a predictive method for in vitro percutaneous absorption

Application of attenuated total reflection Fourier transform infrared imaging and tape-stripping to investigate the three-dimensional distribution of exogenous chemicals and the molecular organization in Stratum corneum

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