Chemometrics-Assisted UV Spectrophotometric Method for Determination of Metformin Hydrochloride and Glyburide in Pharmaceutical Tablets

Abstract

This study aims to estimate simultaneously metformin hydrochloride (MET) and glyburide (GLY) in a multicomponent tablets dosage form by spectrophotometric method using chemometric approaches such as principal component regression (PCR) and partial least-squares regression (PLS). Because of highly overlapped in UV spectra and difference proportions of two active ingredients, the conventional univariate calibration methods was not allowed without previous separation. The linearity ranges used to construct the calibration matrix were selected in the ranges from 40.00 to 200.00 mg L-1 for MET and from 1.00 to 10.00 mg L-1 for GLY. The absorbances were measured in the wavelength range of 200-400 nm, using ethanol as solvent. The resulting UV spectra were subjected to PCR and PLS algorithms and the optimum numbers of principal components (PCs) were selected according to prediction residual error sum of squares (PRESS) values of leave-one out cross-validation. The number of PCs for MET and GLY were found to be 5, 3 by PCR and 5, 3 by PLS, respectively. A set of synthetic mixtures was employed to verify the models and the performance of the models were shown in the values of the root mean square error in prediction (RMSEP). RMSEP values of MET and GLY were 1.806, 0.256 for PCR and 1.802, 0.185 for PLS, respectively. The suitable calibration models were applied to the analysis of these compounds in pharmaceutical formulation.

Chemometrics-assisted spectrophotometry for simultaneous determination of sodium benzoate and citric acid in beverage products

“…Simultaneous analysis of both compounds using conventional spectrophotometry cannot be performed with a good result because sodium benzoate and citric acid have overlapping absorbance UV spectra (Wasito and Phechkrajang, 2015). Spectrophotometry with chemometrics approach was one of the strategies to overcome the problem (Phechkrajang et al, 2011;Sratthaphut and Ruttanakorn, 2015). Overlapping UV absorbance data from measurements of compound mixtures with spectrophotometry could be used and processed by chemometric models for the determination of each compound simultaneously (El-Zaher et al, 2017;Patel et al, 2014;Wasito et al, 2018).…”