Chemometric optimisation of enzymatic hydrolysis of beechwood xylan to target desired xylooligosaccharides

Díaz-Arenas, Gloria Lizeth; Lebanov, Leo; Rodríguez, Estrella Sanz; Muhammad, Sadiq; Paull, Brett; Garnier, Gil; Tanner, Joanne

doi:10.1016/j.biortech.2022.127041

Cited by 3 publications

(2 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The progress of XOS formation was also followed by anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), which offers the highest resolution for this kind of mixtures [17,23]. The chromatograms at 3 h and 48 h are shown in Fig.…”

Section: Production Of Xos By Enzymatic Hydrolysis Of Beechwood Xylanmentioning

confidence: 99%

See 1 more Smart Citation

Enzymatic bioconversion of beechwood xylan into the antioxidant 2′-O-α-(4-O-methyl-D-glucuronosyl)-xylobiose

Míguez

Fernandez-Polo

Santos-Moriano

et al. 2022

Biomass Conv. Bioref.

View full text Add to dashboard Cite

Acidic xylooligosaccharides (XOS), also called aldouronics, are hetero-oligomers of xylose randomly branched with 4-O-methyl-D-glucuronic acid residues linked by α(1 → 2) bonds, which display bioactive properties. We have developed a simple and integrated method for the production of acidic XOS by enzymatic hydrolysis of a glucurono-xylan from beechwood. Among the enzymes screened, Depol 670L (a cellulolytic preparation from Trichoderma reesei) displayed the highest activity (70.3 U/mL, expressed in reducing xylose equivalents). High-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) analysis revealed the formation of a neutral fraction (corresponding to linear XOS, mainly xylose and xylobiose) and a group of more retained products (acidic XOS), which were separated using strong anion-exchange cartridges. The acidic fraction contained a major product, characterized by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and mono- and two-dimensional nuclear magnetic resonance spectroscopy (NMR) as 2′-O-α-(4-O-methyl-α-D-glucuronosyl)-xylobiose (X2_MeGlcA). Starting from 2 g of beechwood xylan, 1.5 g of total XOS were obtained, from which 225 mg (11% yield) corresponded to the aldouronic X2_MeGlcA. The acidic XOS exhibited higher antioxidant activity (measured by the ABTS·+ discoloration assay) than xylan, whilst neutral XOS displayed no antioxidant activity. This work demonstrates that it is possible to obtain a safe and natural antioxidant by enzymatic biotransformation of hardwood hemicellulose.

show abstract

Section: Production Of Xos By Enzymatic Hydrolysis Of Beechwood Xylanmentioning

confidence: 99%

“…However, due to intrinsic xylan complexity, a synergistic action of several enzymes (endoxylanases, β-xylosidases, arabinofuranosidases, α-glucuronidases, etc.) is required to achieve the complete breakdown of the polymer [17,18].…”

Section: Introductionmentioning

confidence: 99%