Chemokine Receptor 2 (CXCR2) Gene Variants and Their Association with Periodontal Bacteria in Patients with Chronic Periodontitis

Abstract

Periodontitis, an inflammatory disease caused by subgingival Gram-negative (G-) bacteria, is linked with loss of the connective tissue and destruction of the alveolar bone. In the regulation of inflammatory response, chemokine receptor 2 (CXCR2), a specific receptor for interleukin-8 and neutrophil chemoattractant, plays an important role. The first aim of this study was to investigate the CXCR2 gene variability in chronic periodontitis (CP) patients and healthy nonperiodontitis controls in the Czech population. The second aim was to find a relation between CXCR2 gene variants and the presence of periodontal bacteria. A total of 500 unrelated subjects participated in this case-control study. 329 CP patients and 171 healthy nonperiodontitis controls were analyzed using polymerase chain reaction techniques for three single-nucleotide polymorphisms (SNPs): +785C/T (rs2230054), +1208T/C (rs1126579), and +1440A/G (rs1126580). A DNA microarray detection kit was used for the investigation of the subgingival bacterial colonization, in a subgroup of CP subjects (N=162). No significant differences in allele, genotype, haplotype, or haplogenotype frequencies of CXCR2 gene variants between patients with CP and healthy controls (P>0.05) were determined. Nevertheless, Aggregatibacter actinomycetemcomitans was detected more frequently in men positive for the C allele of the CXCR2 +785C/T polymorphism (61.8% vs. 41.1%, P<0.05; OR=2.31, 95% CI=1.03-5.20) and for the T allele of the CXCR2 +1208C/T variant (61.8% vs. 38.9%, P<0.05; OR=2.54, 95% CI=1.13-5.71). In contrast, no statistically significant associations of CXCR2 variants with seven selected periodontal bacteria were found in women. Although none of the investigated SNPs in the CXCR2 gene was associated with CP, the CXCR2 gene variants can be associated with subgingival colonization of G- bacteria in men with CP in the Czech population.

Influence of inflammasome NLRP3, and IL1B and IL2 gene polymorphisms in periodontitis susceptibility

“…After multivariate analysis we observed a tendency (limited by the low statistical power) of men to be predisposed to the disease when in the presence of some polymorphisms in the inflammasome, cytokine and cytokine receptor genes, regardless of cigarette use. Previous studies had found a correlation between immune gene polymorphisms and men's susceptibility to PD [77,78], including Southern Brazilians patients [13,79]. Thus, our data corroborates in emphasizing the importance of genetic factors in men's susceptibility to developing PD.…”

Chemokine Receptor 2 (CXCR2) Gene Polymorphisms and Their Association with the Risk of Developing Peri-Implantitis in Chinese Han Population

“…Kavrikova et al analyzed the association of CXCR2 gene variants with periodontal bacteria in patients with chronic periodontitis in the Czech population and found that the C allele of rs2230054 and the T allele of rs1126579 in men were closely associated with Aggregatibacter actinomycetemcomitans, the main etiological agent of periodontitis. 15 In the Brazilian population, among three SNPs of the CXCR2 gene, including rs2230054, rs1126579, and rs1126580, only rs1126580 showed significant association with periodontitis of patients. 26…”

“…14 Kavrikova et al have reported that CXCR2 gene variants are correlated with the subgingival Grannegative bacteria in chronic periodontitis patients, which is the major cause of periodontitis. 15 Therefore, CXCR2 was speculated to be involved in the pathogenesis of periimplantitis.…”

A SNaPshot Assay for Determination of the Mannose-Binding Lectin Gene Variants and an Algorithm for Calculation of Haplogenotype Combinations

“…Conversely, haplogenotypes (combinations of unphased genotypes) have been used in association studies [ 55 ]. Here, a simple online application for the calculation of haplogenotypes by combining determined genotypes of two to ten SNPs has been prepared to facilitate further statistical analyses.…”