Chemokine-like factor 1 promotes the migration of rat primary cortical neurons by the induction of actin polymerization

Wang, Zhenzhen; Yuan, Yu‐He; Zhang, Yi; Chu, Shifeng; Han, Ning; Chen, Nai‐Hong

doi:10.1097/wnr.0000000000000252

Cited by 10 publications

(7 citation statements)

References 23 publications

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“…Previous studies have reported that CKLF1 promotes the migration of rat primary cortical neurons (34,35), indicating the chemotactic activity of CKLF1. Overexpression of CKLF2 induces the proliferation and survival of skeletal muscle cells (32).…”

Section: Cklf1 Is a Recently Discovered Chemokine With Broad-spectrummentioning

confidence: 84%

“…Several studies have demonstrated that the expression of CKLF1 increases in inflammation and autoimmune diseases like rheumatoid arthritis and asthma (31,33). Furthermore, CKLF1 displays chemotactic activities in neuroblastoma SHSY5Y cells and promotes the migration of rat primary cortical neurons (34,35). However, there is no report about the relationship between CKLF1 and cancers, including HCC.…”

Section: Cklf1 Is a Recently Discovered Chemokine With Broad-spectrummentioning

confidence: 99%

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CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC

Liu

Zhou

et al. 2019

Clinical Cancer Research

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Purpose: Hepatocellular carcinoma (HCC), one of the most common and deadliest malignancies worldwide, has a poor prognosis, owing to its high potential for vascular invasion and metastasis and the lack of biomarkers for early diagnosis. Thus, it must be a crucial factor for investigating therapeutic strategies for HCC to identify the functional molecular targets. Here, we reported a novel chemokine, CKLF1, that might act as a pivotal modulator in the invasion and metastasis of HCC and could serve as an attractive target for cancer therapy.Experimental Design: Bioinformatics analysis, PCR, Western blotting, and IHC were performed to detect the expression of CKLF1 in HCC. The function of CKLF1 was demonstrated by a series of in vitro and in vivo experiments. Pharmacologic treatment, flow cytometry, and Western blotting were carried out to demonstrate the potential mechanisms of CKLF1.Results: We proved that CKLF1 was overexpressed in HCC tissues and was related to tumor stage, vascular invasion, and patient survival. Then, functional assays showed that CKLF1 promoted hepatocellular carcinogenesis and metastatic potential. Finally, the IL6/STAT3 signaling pathway was involved in the mechanistic investigation. The results demonstrated that CKLF1 enhanced the progression of HCC and prevented doxorubicin-induced apoptosis through activating the IL6/ STAT3 pathway.Conclusions: These data showed that CKLF1 inhibited apoptosis and promoted malignant transformation through the IL6/STAT3 pathway, and ultimately enhanced the development and metastasis of HCC. Thus, our work revealed that CKLF1 was a significant prognostic factor of HCC and might be a potential molecular therapeutic target for HCC.

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Section: Cklf1 Is a Recently Discovered Chemokine With Broad-spectrummentioning

confidence: 84%

Section: Cklf1 Is a Recently Discovered Chemokine With Broad-spectrummentioning

confidence: 99%

CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC

Liu

Zhou

et al. 2019

Clinical Cancer Research

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“…These methods provide faster and more reproducible responses on the functional role of genes and proteins implicated in neuronal migration compared to ex vivo and in vivo methods. These methods are also suitable to study intracellular signaling pathways related to cell migration such as cytoskeleton remodeling and cell-matrix adhesion properties [26], especially when combined with live imaging.…”

Section: How To Study Neuronal Migration In the Cerebral Cortexmentioning

confidence: 99%

In Vitro, Ex Vivo and In Vivo Techniques to Study Neuronal Migration in the Developing Cerebral Cortex

et al. 2017

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Neuronal migration is a fundamental biological process that underlies proper brain development and neuronal circuit formation. In the developing cerebral cortex, distinct neuronal populations, producing excitatory, inhibitory and modulatory neurotransmitters, are generated in different germinative areas and migrate along various routes to reach their final positions within the cortex. Different technical approaches and experimental models have been adopted to study the mechanisms regulating neuronal migration in the cortex. In this review, we will discuss the most common in vitro, ex vivo and in vivo techniques to visualize and study cortical neuronal migration.

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“…Besides, their research also found that CKLF1 promoted the migration of primary cortical neurons in rats by means of inducing actin polymerization in a dose-dependent manner. 17 Promoting proliferation and differentiation CKLF1 has proliferation-and differentiation-promoting abilities in many cell types, such as skeletal muscle cells, bone marrow cells, vascular smooth muscle cells, and hematopoietic stem/progenitor cells 8 (Table 1). Han et al 8 found that CKLF1 could promote the proliferation of mouse skeletal muscle cells in vitro and also enhance proliferation and differentiation in vivo.…”

Section: Biological Functions Of Cklf1 Chemotactic Activitymentioning

confidence: 99%

“…Besides, their research also found that CKLF1 promoted the migration of primary cortical neurons in rats by means of inducing actin polymerization in a dose-dependent manner. 17…”

Section: Biological Functions Of Cklf1mentioning

confidence: 99%

Chemokine-like factor 1: A promising therapeutic target in human diseases

Cai

Deng

Ming

et al. 2020

Exp Biol Med (Maywood)

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Chemokines are a family of small molecular-weight proteins, being well-known with their important roles in the process of allergic response, immune regulation, cell proliferation, and differentiation. Almost two decades ago, a novel member of this family, chemokine-like factor 1 (CKLF1) was identified. In recent years, it draws much attention of researchers because of its key roles in many types of tissues and its potential implications in a large number of diseases. Up to now, CKLF1 has been shown to have two main functions: broad-spectrum chemotactic activity, and proliferation- and differentiation-promoting abilities. In this review, we would introduce the basic structural features of CKLF1 and its biological functions, and then elaborate the relationships between CKLF1 and human diseases. The cell signaling pathways CKLF1 may be involved in would be discussed and summarized in details. Furthermore, we present the trials of CKLF1-targeted treatments in animal disease models, hoping to provide a few important insights about CKLF1 to both medical researchers and pharmacy, and finally conclude that CKLF1 is a potent, and very promising, therapeutic target. Impact statement CKLF1, a recently identified chemokine, has been reported by a number of studies to play important roles in quite many diseases. However, the potential pathways that CKLF1 may be involved are not manifested well yet. In our review, we showed the basic molecular structure and major functions of this novel chemokine, and implication in human diseases, such as tumors. To attract more attention, we summarized its signaling pathways and clearly present them in a set of figures. With the overview of the experimental trial of CKLF1-targeting medicines in animal models, we hope to provide a few important insights about CKLF1 to both medical researchers and pharmacy.

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Chemokine-like factor 1 promotes the migration of rat primary cortical neurons by the induction of actin polymerization

Cited by 10 publications

References 23 publications

CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC

CKLF1 Enhances Inflammation-Mediated Carcinogenesis and Prevents Doxorubicin-Induced Apoptosis via IL6/STAT3 Signaling in HCC

In Vitro, Ex Vivo and In Vivo Techniques to Study Neuronal Migration in the Developing Cerebral Cortex

Chemokine-like factor 1: A promising therapeutic target in human diseases

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