Chemodynamics underlying N-acetyl cysteine-mediated bone cement monomer detoxification

Yamada, Masahiro; Ogawa, Takahiro

doi:10.1016/j.actbio.2009.04.027

Cited by 30 publications

(28 citation statements)

References 66 publications

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“…8). It is well documented that prevention of oxidative stress leads to restoration of cellular viability and differentiation in both fibroblasts and osteoblasts [29,[31][32][33][34][35][36]38]. Therefore, it is possible that NAC reinforces or compensates the antioxidant capacity of cells on a scaffold by acting as a GSH supply source (Pathway 2 in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…This strong antioxidant activity of NAC protects cells from oxidative stress caused by various external stimuli [7,30]. In earlier studies, we demonstrated that NAC detoxified oxidative stress-inducing biomaterials such as polymethyl methacrylate resin (PMMA) and organic and inorganic bone biomaterials, allowing maintenance of cell viability and differentiation in dental pulp cells, fibroblasts and osteoblasts in contact with those materials by reduction of oxidative stress [29,[31][32][33][34][35][36][37][38]. Large quantities of direct bone deposition occurred on NAC-incorporated PMMA bone cement implanted into rat femur bone in contrast with sparse bone formation around PMMA cement alone [33].…”

Section: Generally Certain Types Of Staphylococci and Streptococci Smentioning

confidence: 99%

“…This small-molecule compound (molecular weight: 163. 19) can directly scavenge free radicals and toxic compounds with its functional moiety, the sulfhydryl group [7,29]. In addition, NAC is membrane-permeably incorporated into cells and rapidly metabolized into L-cysteine, a precursor of GSH, resulting in maintenance of intracellular redox balance.…”

Section: Generally Certain Types Of Staphylococci and Streptococci Smentioning

confidence: 99%

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The inhibition of infection by wound pathogens on scaffold in tissue-forming process using N-acetyl cysteine

et al. 2011

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Prevention of local infection from wound pathogens such as Staphylococci and Streptococci is crucial for tissue regeneration. N-acetyl cysteine (NAC), an antioxidant amino acid derivative, has anti-microbial potential against various species. This in vitro study evaluated whether NAC prevented bacterial infection of gingival fibroblasts and osteoblasts on a scaffold. N-acetyl cysteine delayed growth of Staphylococcus aureus and Streptococcus pyogenes cultured in brain heart infusion (BHI) broth for 12 hr in an almost dose-dependent manner (2.5, 5.0 or 10.0 mM). The number of rat gingival fibroblasts on collagen scaffolds with bacterial co-incubation was less than 30% of that in cultures without bacterial co-incubation at day 7. However, pre-addition of NAC to the scaffold yielded a number comparable with that in culture without bacteria. Fibroblasts on the scaffold with bacterial co-incubation were small, rounded and filled with bacteria and reactive oxygen species. Pre-addition of NAC, however, resulted in fibroblasts similar to those observed in culture without bacterial co-incubation. N-acetyl cysteine completely prevented devastating suppression of alkaline-phosphatase activity and extracellular matrix mineralization in osteoblastic culture on scaffolds with bacterial co-incubation. These results indicate that NAC can functionalize a scaffold with anti-infective capabilities, thus assisting healing of soft and hard tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Generally Certain Types Of Staphylococci and Streptococci Smentioning

confidence: 99%

Section: Generally Certain Types Of Staphylococci and Streptococci Smentioning

confidence: 99%

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The inhibition of infection by wound pathogens on scaffold in tissue-forming process using N-acetyl cysteine

et al. 2011

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“…However this occurs in PMMA based cements as the sulfhydryl groups from Cys. can inactivate the monomer components [50] . Cys.…”

Section: Resultsmentioning

confidence: 99%

“…Cys. can also eliminate free radicals by enabling the self detoxification of bone cement by preventing free radicals from being released from the material or by enhancing cellular antioxidant defense [50,51] . Although Ker.…”

Section: Resultsmentioning

confidence: 99%

The effect of adding organic polymers on the handling properties, strengths and bioactivity of a Ca–Sr–Zn–Si glass polyalkenoate cement

et al. 2010

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Detoxification of poly(methyl methacrylate) bone cement by natural antioxidant intervention

Choppadandi

Kapusetti

2019

J Biomedical Materials Res

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Poly(methyl methacrylate) (PMMA) bone cement is the most widely used grouting material in the joint arthroplasties and vertebroplasties. The present investigation has been carried out to scavenge the radicals and monomer by addition of an antioxidant to minimize the toxicity of bone cement (BC). The in silico studies were employed to determine the potent natural antioxidant at physiological conditions. The antioxidant methionine demonstrated a strong binding affinity with free radicals and methyl methacrylate (MMA) monomer than cysteine. The designated amount of methionine was optimized by various assay methods and >2% methionine shows strong scavenging capacity in BC. Moreover, the antioxidant‐loaded BC (ABC) demonstrated similar handling, physicochemical and mechanical properties to pristine bone cement. Significantly, the developed formulation shows superior biological characteristics such as cell proliferation (2 ± 1 BC and 6 ± 1 ABC), adhesion (0.32 ± 0.02 BC and 0.54 ± 0.01 ABC), and cell viability (81 ± 2% BC and 93 ± 1% ABC) toward human osteoblast‐like cells (MG‐63). Therefore, the novel antioxidant bone cement is a potential candidate for various orthopedic applications to eliminate the adverse effects, related to residual toxic radical and monomer in bone cement.

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Chemodynamics underlying N-acetyl cysteine-mediated bone cement monomer detoxification

Cited by 30 publications

References 66 publications

The inhibition of infection by wound pathogens on scaffold in tissue-forming process using N-acetyl cysteine

The inhibition of infection by wound pathogens on scaffold in tissue-forming process using N-acetyl cysteine

The effect of adding organic polymers on the handling properties, strengths and bioactivity of a Ca–Sr–Zn–Si glass polyalkenoate cement

Detoxification of poly(methyl methacrylate) bone cement by natural antioxidant intervention

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