Chemistry Study on Protective Effect against·OH-induced DNA Damage and Antioxidant Mechanism of Cortex Magnoliae Officinalis

Protective Effect Against Hydroxyl Radical-induced DNA Damage and Antioxidant Mechanism of [6]-gingerol: A Chemical Study

“…The DPPH• radicalscavenging activity was determined as described. 23 Briefly, 500 μL of DPPH• solution (0.1 mM) was mixed with 250 μL sample 95% ethanol solution with various concentrations. The mixture was kept at room temperature for 30 min, and then the absorbance was measured at 519 nm against 95% ethanol (as blank).…”

“…The mixture was kept in the dark at room temperature for 12 h to allow completion of radical generation, then diluted with 95% ethanol (about 1:50) so that its absorbance at 734 nm was 0.70 ± 0.02. Then, 0.6 mL diluted ABTS ) reducing power capacity was determined based on the method, 23 with a slight modification. In brief, 100 μL CuSO 4 aqueous solution (10 mM), 100 μL neocuproine ethanolic solution (7.5 mM) and 400 μL CH3COONH4 buffer solution (100 mM, pH 7.0) were brought to test tubes with different volumes of samples (0.1 mg/mL, 15-75 μL).…”

Maclurin protects against hydroxyl radical-induced damages to mesenchymal stem cells: Antioxidant evaluation and mechanistic insight

“…The cupric ions (Cu 2+ ) reducing power capacity was determined based on the method [31], with a slight modification. In brief, 12.5 lL CuSO 4 aqueous solution (10 mM), 12.5 lL neocuproine ethanolic solution (7.5 mM) and 50 lL CH 3 COONH 4 buffer solution (100 mM, pH 7.0) were brought to test tubes with different volumes of samples (0.1 mg/mL, 2-10 lL).…”

General Machine Learning Model, Review, and Experimental-Theoretic Study of Magnolol Activity in Enterotoxigenic Induced Oxidative Stress

“…ROS are an important inducer of the pathogenesis of these diseases and the therapeutic function of magnolol is tightly linked to its antioxidant effectiveness. Moreover, it has been reported that magnolol scavenges hydroxyl radical [11], peroxy-nitrite [12] and hydrogen peroxide [13] to reduce or suppress the generation of ROS. Furthermore, there is an additional direct evidence of its anti-oxidative effect on intracellular glutathione depletion [9] or enzymatic system capacity in mice [14].…”

“…Accumulating evidence indicates that magnolol may play an important role in the treatment of various diseases due to its anticancer [6], anti-clastogenic [7], anti-inflammatory effects [8], and antioxidant activity, etc. [9][10][11][12][13][14][15][16][17][18]. It is noteworthy that the relevant magnolol studies have mainly focused on the evaluation of its beneficial effects against oxidative radicals in vitro.…”