Chemistry and Biological Activity of Triterpenoid Glycosides from Medicago Sativa

Timbekova, A. É.; Исаев, М. И.; Abubakirov, N. K.

doi:10.1007/978-1-4613-0413-5_14

Cited by 21 publications

(22 citation statements)

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“…For example, the growth of C. albicans, Candida utilis, Bacillus mesenterius and Pseudomonas lachrymans was not inhibited by the tested phytocompounds, whereas whole-root extract and the glucopyranoside of medicagenic acid limited the growth of Candida tropicalis. All tested preparations also fully suppressed the development of Agrobacterium tumefaciens and Corynebacterium michiganense (Timbekova et al, 1996). In other studies, Saccharomyces cerevisiae appeared to be the most susceptible among all tested fungi (including C. albicans, C. tropicalis and Candida laurentii) to saponins, mainly to sapogenin mixtures and medicagenic acid .…”

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confidence: 62%

“…For many years saponins have been described as compounds influencing animals and humans via immunostimulatory, antiinflammatory, hypocholesterolaemic, antioxidative and anticancerogenic activity. They also reduce the growth of some insects, protozoans and micro-organisms (Coleman et al, 2010;Francis et al, 2002;Sparg et al, 2004;Timbekova et al, 1996;Udgirkar et al, 2013;Weng et al, 2010). Commercially available saponins are mainly extracted from Yucca schidigera and Quillaja saponaria.…”

Section: Introductionmentioning

confidence: 99%

“…Even the extraction method can lead to different constitutions and thus various biological properties. According to the research performed by Timbekova et al (1996), the medicagenic acid glucopyranoside as well as hederagenin glycosides and their derivatives (containing medicagenic acid or hederagenin as aglycones) are the main components of M. sativa extracts. However, the antimicrobial activity of crude saponin mixtures obtained from M. sativa aerial parts, roots and their individual components was still very diverse, and depended on both the type of extract/compound and the tested micro-organism.…”

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New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

Sadowska

Budzyńska

Więckowska-Szakiel

et al. 2014

Journal of Medical Microbiology

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The antifungal activity of the saponin-rich fractions (SFs) from Medicago sativa (aerial parts and roots) and Saponaria officinalis (used as a well-known source of plant saponins) against Candida albicans reference and clinical strains, their yeast-to-hyphal conversion, adhesion, and biofilm formation was investigated. Direct fungicidal/fungistatic properties of the tested phytochemicals used alone, as well as their synergy with azoles (probably resulting from yeast cell wall instability) were demonstrated. Here, to the best of our knowledge, we report for the first time the ability of saponin-rich extracts of M. sativa and S. officinalis to inhibit C. albicans germ tube formation, limit hyphal growth, reduce yeast adherence and biofilm formation, and eradicate mature (24 h) Candida biofilm. Moreover, M. sativa SFs (mainly obtained from aerial parts), in the range of concentrations which were active modulators of Candida virulence factors, exhibited low cytotoxicity against the mouse fibroblast line L929. These properties seem to be very promising in the context of using plant-derived SFs as potential novel antifungal therapeutics supporting classic drugs or as ingredients of disinfectants. INTRODUCTIONCandida albicans is probably the best known and at the same time the most effective opportunistic fungal pathogen of humans and animals. It constitutes a commensal microflora in the gastrointestinal and genitourinary tracts of .70 % of humans. However, in certain circumstances C. albicans could be pathogenic to critically ill, immunocompromised patients or even to healthy persons. It causes a diverse range of pathologies -from local cutaneous and mucosal infections (e.g. candidiasis of nail shafts and nails, oropharyngeal candidiasis, intestinal candidiasis) to lifethreatening systemic infections, such as candidaemia, fungal pneumonia, meningitidis or endocarditis (Cannon et al., 2009;Kabir et al., 2012;Kabir & Ahmad, 2013;Sardi et al., 2013; Tlamçani & Er-rami, 2013). Endogenous candidaemia and the carrier state are the main mechanisms of Candida spp. spread and transmission, although exogenous infections are also possible (Mathé & van Dijck, 2013;Sardi et al., 2013). The pathogenicity and high invasiveness of C. albicans arise from the broad range of its virulence factors. These include tissue-damaging hydrolytic enzymes: proteases (mainly secreted aspartic proteinases), phospholipases and haemolysins, but first of all the agents/abilities contributing to its strong adherence (e.g. two main C. albicans adhesions: agglutinin-like sequence and hyphal wall protein-1), biofilm formation and the transformation of morphological forms (yeast-to-hyphal). Also, the ability of C. albicans to survive in various anatomical body sites and to evade host defences seems to be essential (Chai et al., 2009;Chandra et al., 2001;Gropp et al., 2009;Sardi et al., 2013).Invasive candidiasis usually results from tissue colonization by yeast and formation of biofilm -the structured multicellular microbial communities embedded in a self-...

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confidence: 62%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

Sadowska

Budzyńska

Więckowska-Szakiel

et al. 2014

Journal of Medical Microbiology

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“…The inhibitory effects of these glycosides on cotton and cabbage seed germinations have also been tested (Timbekova, 1995).…”

Section: B Subtilis Staphylococcus Aureus and Enterococcus Faecalis)mentioning

confidence: 99%

Phytochemical and pharmacological potential ofMedicago sativa: A review

Bora¹,

Sharma²

2010

Pharmaceutical Biology

137

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“…Especially, since a health curative effect is well-known in traditional rustic medicine of some legumes, e.g. Medicago sativa L., Trifolium pratense L., and also from several systematic studies (Chang et al 1978;Sukhinin et al 1981;Timbekova et al 1996;Avato et al 2006;Booth et al 2006;Wang et al 2008;Sabudak & Guler 2009). It is known that a group of peptide inhibitors isolated from seeds of Medicago sp.…”

Section: Introductionmentioning

confidence: 99%

Proteinase inhibition and antioxidant activity of selected forage crops

et al. 2010

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The hypothesis of the possible therapeutic potential of selected species of forage crops is discussed. Extracts from genotypes of Anthyllis sp., Astragalus sp., Coronilla sp., Lotus sp., Medicago sp., Melilotus sp., Onobrychis sp. and Trifolium sp. were prepared and tested for proteinase inhibition and antioxidant activities. We found that Trifolium pratense accession POLKIE99-3 expressed the highest relative trypsin inhibition activity (80.0%) compared to standards. The highest thrombin inhibition activity (81.4%) was detected in the Medicago sativa old cultivar Hodonínka, whereas the highest relative urokinase inhibition activity (62.5%) was expressed by the local population Nitranka. Relatively high antioxidant properties of Trifolium sp. accessions, Trifolium pratense genetic resources SVKZAH98-40, were of interest. Results of this study confirmed that there are significant differences in proteinase inhibition and antioxidant activity among important selected agricultural crops. The present paper may also be the starting point of the research aimed for development of new functional food and nutraceuticals, and/or of the research focused on new secondary metabolites with potential as bioactive compounds.Key words: trypsin; thrombin; urokinase; inhibition; antioxidant; activity; forage crops.Abbreviations: AOX, antioxidant activity; BBI, Bowman-Birk inhibitor; DMSO, dimethyl sulphoxide; Rel%BT, relative percentage value of activity to standardized black tea extract; Rel%Q, relative percentage value of activity to standard quercetin; SHE, sunflower oil hydrolysate ethanol; SITh, selectivity index of thrombin; SIU, selectivity index of urokinase; ThIA, thrombin inhibition activity; TrIA, trypsin inhibition activities; UIA, urokinase inhibition activity.

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Chemistry and Biological Activity of Triterpenoid Glycosides from Medicago Sativa

Cited by 21 publications

References 10 publications

New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

Phytochemical and pharmacological potential ofMedicago sativa: A review

Proteinase inhibition and antioxidant activity of selected forage crops

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