ChemInform Abstract: Molecular Characterization of the Ligand‐Receptor Interaction of Neuropeptide Y

Ingenhoven, Nikolaus; Beck‐Sickinger, Annette G.

doi:10.1002/chin.199952299

Cited by 2 publications

(3 citation statements)

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“…, 2004; Zukowska, 2005). Five NPY receptors have been classified: Y 1 , Y 2 , Y 4 , Y 5 and Y 6 (Ingenhoven and Beck‐Sickinger, 1999; all receptor nomenclature follows Alexander et al. , 2008).…”

Section: Introductionmentioning

confidence: 99%

“…It also acts as a vasoconstrictor and a vascular mitogen, effects that appear to be involved in the development of atherosclerosis via particular postsynaptic NPY receptors (Pons et al, 2004;Zukowska, 2005). Five NPY receptors have been classified: Y 1, Y2, Y4, Y5 and Y6 (Ingenhoven and Beck-Sickinger, 1999; all receptor nomenclature follows Alexander et al, 2008). Their activation results in adenylate cyclase inhibition and a decrease in cAMP via Pertussis toxin-sensitive G-proteins.…”

Section: Introductionmentioning

confidence: 99%

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Receptors for NPY and PACAP differ in expression and activity during adipogenesis in the murine 3T3‐L1 fibroblast cell line

Gericke¹,

Kosacka²,

Koch³

et al. 2009

British J Pharmacology

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Background and purpose: Neuropeptides are involved in the regulation of food intake in the central nervous system, but they might also act on peripheral fat tissue via neuropeptide receptors. Experimental approach: We investigated the receptor expression and activity of pituitary adenylate cyclase-activating polypeptide (PACAP) and of neuropeptide Y at the mRNA and protein levels in the 3T3-L1 fibroblast line during differentiation into adipocytes. Intracellular calcium concentration was measured by calcium imaging. Key results: The PACAP receptors PAC1 and VPAC2 as well as the neuropeptide Y1 receptor were expressed at the mRNA level in fibroblasts, pre-adipocytes and adipocytes. The mRNA profile of the PAC1 receptor isoforms showed the HOP sequence, whereas the HIP-isoform was present in subconfluent 3T3-L1 fibroblasts only. At the protein level, the mature 3T3-L1 adipocytes produced the PAC1 and Y1 receptors; only the PAC1 receptor showed carbohydrate residues. Both neuropeptides induced an increase of intracellular calcium in mature adipocytes, which was absent in the precursor cells. These changes in calcium were mediated by Y1 and PAC1 receptors as demonstrated by the effects of specific receptor agonists and antagonists. Conclusions and implications:As the PAC1-HOP receptor variant seems to be responsible for PACAP-mediated calcium influx in many cell types, the HOP sequence might also mediate the increase in intracellular calcium in adipocytes. Because a high intracellular calcium level is associated with lipogenesis, peptidergic innervation of adipose tissue might be involved in stress-induced obesity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Receptors for NPY and PACAP differ in expression and activity during adipogenesis in the murine 3T3‐L1 fibroblast cell line

Gericke¹,

Kosacka²,

Koch³

et al. 2009

British J Pharmacology

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“…The PYY gene encodes for peptide YY, a gut endocrine factor that circulates after meals [ 30 ]. NPY encodes for neuropeptide Y, an abundant brain and autonomic neurotransmitter [ 31 ] and potent orexigen [ 32 ]. NPY5R encodes for one of five neuropeptide Y receptors studied in relation to energy balance [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Physiogenomic comparison of human fat loss in response to diets restrictive of carbohydrate or fat

et al. 2008

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BackgroundGenetic factors that predict responses to diet may ultimately be used to individualize dietary recommendations. We used physiogenomics to explore associations among polymorphisms in candidate genes and changes in relative body fat (Δ%BF) to low fat and low carbohydrate diets.MethodsWe assessed Δ%BF using dual energy X-ray absorptiometry (DXA) in 93 healthy adults who consumed a low carbohydrate diet (carbohydrate ~12% total energy) (LC diet) and in 70, a low fat diet (fat ~25% total energy) (LF diet). Fifty-three single nucleotide polymorphisms (SNPs) selected from 28 candidate genes involved in food intake, energy homeostasis, and adipocyte regulation were ranked according to probability of association with the change in %BF using multiple linear regression.ResultsDieting reduced %BF by 3.0 ± 2.6% (absolute units) for LC and 1.9 ± 1.6% for LF (p < 0.01). SNPs in nine genes were significantly associated with Δ%BF, with four significant after correction for multiple statistical testing: rs322695 near the retinoic acid receptor beta (RARB) (p < 0.005), rs2838549 in the hepatic phosphofructokinase (PFKL), and rs3100722 in the histamine N-methyl transferase (HNMT) genes (both p < 0.041) due to LF; and the rs5950584 SNP in the angiotensin receptor Type II (AGTR2) gene due to LC (p < 0.021).ConclusionFat loss under LC and LF diet regimes appears to have distinct mechanisms, with PFKL and HNMT and RARB involved in fat restriction; and AGTR2 involved in carbohydrate restriction. These discoveries could provide clues to important physiologic mechanisms underlying the Δ%BF to low carbohydrate and low fat diets.

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ChemInform Abstract: Molecular Characterization of the Ligand‐Receptor Interaction of Neuropeptide Y

Cited by 2 publications

References 1 publication

Receptors for NPY and PACAP differ in expression and activity during adipogenesis in the murine 3T3‐L1 fibroblast cell line

Receptors for NPY and PACAP differ in expression and activity during adipogenesis in the murine 3T3‐L1 fibroblast cell line

Physiogenomic comparison of human fat loss in response to diets restrictive of carbohydrate or fat

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