ChemInform Abstract: Antimicrobial Peptides for Therapeutic Applications

Seo, Min‐Duk; Won, Hyung‐Sik; Kim, Ji‐Hun; Mishig‐Ochir, Tsogbadrakh; Lee, Bong‐Jin

doi:10.1002/chin.201311218

Cited by 10 publications

(16 citation statements)

References 1 publication

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“…5 Because this mode of physical action occurs rapidly, it is difficult for pathogens to develop an effective means of resistance. 6,7 Thus, AMPs are highly active against a range of pathogens 8,9 and hold great potential as peptide drugs against virulent and resistant varieties.…”

Section: Introductionmentioning

confidence: 99%

“…They often suffer from low efficiency, potential toxicity and side effects, proteolytic degradation, and relatively high costs. 9 Extensive studies have focused on developing new AMPs by either modifying natural ones or following the strategy of artificial design to produce efficient and cost-effective versions. Among other approaches, one key factor lies in altering amino acid sequences to produce AMPs with different amphiphilicities.…”

Section: Introductionmentioning

confidence: 99%

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Hydrophobic Control of the Bioactivity and Cytotoxicity of de Novo-Designed Antimicrobial Peptides

Gong

Zhang

et al. 2019

ACS Appl. Mater. Interfaces

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Antimicrobial peptides (AMPs) can target bacterial membranes and kill bacteria through membrane structural damage and cytoplasmic leakage. A group of surfactant-like cationic AMPs was developed from substitutions to selective amino acids in the general formula of G(IIKK)3I-NH2, (called G3, a de novo AMP), to explore the correlation between AMP hydrophobicity and bioactivity. A threshold surface pressure over 12 mN/m was required to cause measurable antimicrobial activity and this corresponded to a critical AMP concentration. Greater surface activity exhibited stronger antimicrobial activity but had the drawback of worsening hemolytic activity. Small unilamellar vesicles (SUVs) with specific lipid compositions were used to model bacterial and host mammalian cell membranes by mimicking the main structural determinants of the charge and composition. Leakage from the SUVs of encapsulated carboxyfluorescein measured by fluorescence spectroscopy indicated a negative correlation between hydrophobicity and model membrane selectivity, consistent with measurements of the zeta potential that demonstrated the extent of AMP binding onto model SUV lipid bilayers. Experiments with model lipid membranes thus explained the trend of minimum inhibitory concentrations and selectivity measured from real cell systems and demonstrated the dominant influence of hydrophobicity. This work provides useful guidance for the improvement of the potency of AMPs via structural design, whilst taking due consideration of cytotoxicity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydrophobic Control of the Bioactivity and Cytotoxicity of de Novo-Designed Antimicrobial Peptides

Gong

Zhang

et al. 2019

ACS Appl. Mater. Interfaces

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“…6−8 AMPs are mostly short, cationic peptides that interact with specific constituents of the bacterial cell envelope, resulting in depolarization, destabilization, and disruption of the bacterial plasma membrane, leading to bacterial cell death within minutes. 6,8,9 Due to the rapid and nonspecific mechanisms of action of the AMPs, it is less likely that bacteria develop resistance toward them. 6,7 However, their low stability in biological media, together with their interactions with chemical and biological components in complex environments, limit their broader application.…”

Section: Introductionmentioning

confidence: 99%

Bioinspired Antimicrobial Coatings from Peptide-Functionalized Liquid Crystalline Nanostructures

Zabara

Ren

Amenitsch

et al. 2021

ACS Appl. Bio Mater.

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Surface-associated microbial infections and contaminations are a major challenge in various fields including the food and health sectors. This study demonstrates the design of antimicrobial coatings based on the self-assembly of the food-grade amphiphilic lipid glycerol monooleate with the human cathelicidin-derived antimicrobial peptide LL-37. Structural properties of the coating and their alterations with composition were studied using advanced experimental methods including synchrotron grazing-incidence small-angle X-ray scattering and ellipsometry. The integration of the LL-37 and its potential release from the nanostructured films into the surrounding solution was characterized with confocal Raman microscopy. Additional biological evaluation studies with clinically relevant bacterial strains, namely, Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive), were performed to investigate the antimicrobial activity of the coatings. Significant killing activity of the coating was found against both bacterial strains. The presented findings contribute to the fundamental understanding of lipid–peptide self-assembly on the surface and may open up a promising strategy for designing simple, sustainable antimicrobial coatings for medical and food applications.

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“…6 As compared with leaching-based bactericidal surfaces, such as silver ions 7 and antibiotics, 8 nonleaching bactericidal materials kill bacteria on contact 9 and thus avoid the gradual loss of antibacterial efficiency, eliminate environmental pollution, and alleviate bacterial resistance. 10 Nonleaching bactericidal materials can be fabricated by immobilizing bactericidal components on the surface such as quaternary ammonium groups, 11 quaternary phosphonium groups, 12 antibacterial peptides, 13 lysozymes, 14 and chitosan derivatives. 15 These substances are able to disrupt the membrane structure of bacterial cell and kill bacteria.…”

Section: Introductionmentioning

confidence: 99%

Initiated Chemical Vapor Deposition of Graded Polymer Coatings Enabling Antibacterial, Antifouling, and Biocompatible Surfaces

Song

et al. 2020

ACS Appl. Mater. Interfaces

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We report initiated chemical vapor deposition of model-graded polymer coatings enabling antibacterial, antifouling, and biocompatible surfaces. The graded coating was constructed by a bottom layer consisting of bactericidal poly(dimethyl amino methyl styrene) and a surface layer consisting of both dimethyl amino methyl styrene (DMAMS) and hydrophilic vinyl pyrrolidone (VP) moieties. Fourier transform infrared spectra showed existence of both DMAMS and VP in the coating with DMAMS as the major component, while X-ray photoelectron spectroscopy analysis and water contact angle measurement revealed a VP-enriched coating surface. The resultant coating exhibited more than 99.9% killing rate against both Gram-negative Escherichia coli and Gram-positive Bacillus subtilis despite the incorporation of VP on the surface. We believe that such bactericidal capability resulted because of its high surface zeta potential, which could be originated from the DMAMS units distributed both on the top surface and underneath. The graded coating achieved more than 85% bacterial fouling resistance than the pristine substrate, as well as improved biocompatibility, owing to the abundant surface lactam groups from the VP moiety. Furthermore, the graded coating maintained good bactericidal capability after multicycle challenges of bacterial solutions and was durable against continuous rigorous washing, suggesting potential applications in biomedical devices.

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ChemInform Abstract: Antimicrobial Peptides for Therapeutic Applications

Abstract: Review: 71 refs.

Cited by 10 publications

References 1 publication

Hydrophobic Control of the Bioactivity and Cytotoxicity of de Novo-Designed Antimicrobial Peptides

Hydrophobic Control of the Bioactivity and Cytotoxicity of de Novo-Designed Antimicrobial Peptides

Bioinspired Antimicrobial Coatings from Peptide-Functionalized Liquid Crystalline Nanostructures

Initiated Chemical Vapor Deposition of Graded Polymer Coatings Enabling Antibacterial, Antifouling, and Biocompatible Surfaces

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