Bioinspired Antimicrobial Coatings from Peptide-Functionalized Liquid Crystalline Nanostructures

Abstract

Surface-associated microbial infections and contaminations are a major challenge in various fields including the food and health sectors. This study demonstrates the design of antimicrobial coatings based on the self-assembly of the food-grade amphiphilic lipid glycerol monooleate with the human cathelicidin-derived antimicrobial peptide LL-37. Structural properties of the coating and their alterations with composition were studied using advanced experimental methods including synchrotron grazing-incidence small-angle X-ray scattering and ellipsometry. The integration of the LL-37 and its potential release from the nanostructured films into the surrounding solution was characterized with confocal Raman microscopy. Additional biological evaluation studies with clinically relevant bacterial strains, namely, Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive), were performed to investigate the antimicrobial activity of the coatings. Significant killing activity of the coating was found against both bacterial strains. The presented findings contribute to the fundamental understanding of lipid–peptide self-assembly on the surface and may open up a promising strategy for designing simple, sustainable antimicrobial coatings for medical and food applications.

Human Antimicrobial Peptide Triggered Colloidal Transformations in Bacteria Membrane Lipopolysaccharides

“…[ 9a ] Further, these lipid–AMP complexes exhibited much higher antibacterial activity against gram‐negative bacteria strains, which contain lipopolysaccharides (LPS) on their surface, than gram‐positive strains, where LPS is absent. [ 9b,11 ] Hence, the encapsulation of LL‐37 into lipid nanocarriers appears to modify the interaction with the LPS on the outer membrane of the gram‐negative bacteria.…”

Bioactive Salicylic Acid Containing Coating for Dental Implants to Combat Infection and Inflammation

“…SA is a well‐known anti‐bacterial agent with a minimum inhibitory concentration of 250–500 µg mL −1 (1.81–3.6 × 10 × 10 −3 m ) against S. aureus . [ 47 ] The SA containing surface of Pda + SA ester fabricated in this work inhibited the growth of S. aureus in a touch assay [ 48 ] ( Figure A). Quantitative analysis of the antibacterial activity revealed that Pda+ SA ester coating completely eliminated bacterial growth, whereas the control samples of uncoated glass and Pda + glass led to approximately 941 ± 634 and 2222 ± 1464 CFU mL −1 , respectively (Figure 3B).…”

Peptide-based self-assembled monolayers (SAMs): what peptides can do for SAMs and vice versa

“…Although GISAXS has not been applied for the characterization of peptide-SAMs, it was recently used to evaluate the structural properties of a lipid/AMP self-assembled layer on silicon substrate, based on different ratios of the glycerol monooleate (lipid) and the LL-37 AMP. 212…”