Human Antimicrobial Peptide Triggered Colloidal Transformations in Bacteria Membrane Lipopolysaccharides

Hong, Linda; Amenitsch, Heinz; Salentinig, Stefan

doi:10.1002/smll.202104211

Cited by 14 publications

(17 citation statements)

References 66 publications

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“…The SAXS data of the GMO cubosomes in the absence of RL between pH 3.0–9.0 showed the presence of three Bragg peaks corresponding to the 110, 200, and 211 hkl reflections of the inverse bicontinuous cubic Im3m structure in agreement with previous reports (Figure S4, Supporting Information). [ 23 ] The calculated lattice parameter, a Im 3 m , was 11.7 ± 0.1 nm at all the studied pH values, as found previously ( Figure ). [ 16d,24 ]…”

Section: Resultssupporting

confidence: 84%

Biocompatible Rhamnolipid Self‐Assemblies with pH‐Responsive Antimicrobial Activity

Kadakia,

Valentin,

Hong

et al. 2023

Adv Healthcare Materials

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There is an urgent need for alternative antimicrobial materials due to the growing challenge of bacteria becoming resistant to conventional antibiotics. This study demonstrates the creation of a biocompatible pH‐switchable antimicrobial material by combining bacteria‐derived rhamnolipids (RL) and food‐grade glycerol monooleate (GMO). The integration of RL into dispersed GMO particles, with an inverse‐type liquid crystalline cubic structure in the core, leads to colloidally stable supramolecular materials. The composition and pH‐triggered structural transformations are studied with small‐angle X‐ray scattering, cryogenic transmission electron microscopy, and dynamic light scattering. The composition‐structure‐activity relationship is analyzed and optimized to target bacteria at acidic pH values of acute wounds. The new RL/GMO dispersions reduce Staphylococcus aureus (S. aureus) populations by 7‐log after 24 h of treatment with 64 µg mL−1 of RL and prevent biofilm formation at pH = 5.0, but have no activity at pH = 7.0. Additionally, the system is active against methicillin‐resistant S. aureus (MRSA) with minimum inhibitory concentration of 128 µg mL−1 at pH 5.0. No activity is found against several Gram‐negative bacteria at pH 5.0 and 7.0. The results provide a fundamental understanding of lipid self‐assembly and the design of lipid‐based biomaterials, which can further guide the development of alternative bio‐based solutions to combat bacteria.

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Section: Resultssupporting

confidence: 84%

Biocompatible Rhamnolipid Self‐Assemblies with pH‐Responsive Antimicrobial Activity

Kadakia,

Valentin,

Hong

et al. 2023

Adv Healthcare Materials

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“…However, it was very difficult to observe the PCHM-X effect on the LPS structures because LPS does not have any ordered structures. Indeed, two broad reflections from the multilamellar structure were observed in the SAXS profile, and a broad amorphous halo peak by the disordered alkyl chains was observed in the WAXS profile . The difference in the d -spacings of pure LPS (0.47 nm) and LPS + PCHM-S/PCHM-SO/PCHM-SO 2 /PCHM-O mixtures (0.48, 0.49, 0.48, and 0.48 nm, respectively) was very small, which might be within the experimental error range or may indicate that the distance between the LPS alkyl chains increases slightly following interaction with the PCHM-Xs (Table S9).…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Citronellol-Derived Antibacterial Polymers and Effect of Thioether, Sulfoxide, Sulfone, and Ether Functional Groups on Their Bactericidal Activity

2023

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Citronellol-derived methacrylate polymers with four side chain interconnecting groups of different polarities (PCHM-Xs) are synthesized to prepare bio-based bactericidal polymers. The PCHM-X bactericidal activities are significantly affected by the different functional groups determining the interaction between the polymer chain and bacterial membrane that disrupts the membrane integrity causing cytoplasmic leakage, the main contributor to the polymer antibacterial properties. PCHM-SO, with a large dipole moment and strong hydrogen bonding ability, shows the best bactericidal activity among PCHM-Xs due to the strongest interactions with the bacterial membrane components. A sulfoxide-group-containing polymer with excellent bactericidal activity (99.99%) and the systematic study of the effect of different functional groups on the bactericidal activity are first reported herein.

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“…The checkerboard microdilution method was used to evaluate the effects of LPS and phospholipids (Sigma Aldrich, Saint Louis, MO, USA) on the antibacterial activity of peptides [ 24 ]. Phospholipids include phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and cardiolipin (CL) [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Truncated Pleurocidin Derivative with High Pepsin Hydrolysis Resistance to Combat Multidrug-Resistant Pathogens

et al. 2022

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The global prevalence of antimicrobial resistance calls for the development of novel antimicrobial agents, particularly for these orally available drugs. Structural modifications of the natural antimicrobial peptides (AMPs) provide a straightforward approach to develop potent antimicrobial agents with high specificity and low toxicity. In this study, we truncated 11-amino-acids at the C-terminus of Pleurocidin, an AMP produced by Pleuronectes americanus, and obtained four peptide analogues termed GK-1, GK-2, GK-3 and GK-4. Minimum inhibitory concentration (MIC) tests showed that GK-1 obtained by direct truncation of Pleurocidin has no antibacterial activity, while GK-2, GK-3 and GK-4 show considerable antibacterial activity with Pleurocidin. Notably, GK-4 displays rapid bacteriostatic activity, great stability and low hemolysis, as well as enhanced hydrolytic resistance to pepsin treatment. Mechanistic studies showed that GK-4 induces membrane damage by interacting with bacterial membrane-specific components, dissipates bacterial membrane potential and promotes the generation of ROS. SEM and CD analysis further confirmed the ability of GK-4 to resist pepsin hydrolysis, which may be attributed to its stable helicity structure. Collectively, our findings reveal that GK-4 is a potential orally available candidate to treat infections caused by multidrug-resistant pathogens.

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Human Antimicrobial Peptide Triggered Colloidal Transformations in Bacteria Membrane Lipopolysaccharides

Cited by 14 publications

References 66 publications

Biocompatible Rhamnolipid Self‐Assemblies with pH‐Responsive Antimicrobial Activity

Biocompatible Rhamnolipid Self‐Assemblies with pH‐Responsive Antimicrobial Activity

Synthesis of Citronellol-Derived Antibacterial Polymers and Effect of Thioether, Sulfoxide, Sulfone, and Ether Functional Groups on Their Bactericidal Activity

Truncated Pleurocidin Derivative with High Pepsin Hydrolysis Resistance to Combat Multidrug-Resistant Pathogens

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