Chemiluminescent Ion Sensing Platform Based on Ionophores

Abstract

We report here an ionophore-based chemiluminescent platform for the selective detection of ions. This method functions on the basis of the ion-exchange between the target ion and the divalent organic cation lucigenin, which luminesces after reacting with hydrogen peroxide in alkaline conditions. Using the K+ ionophore valinomycin, chemiluminescent detection of K+ (from 10 nM to 1 M) was performed on both dichloromethane solutions and polymeric films. While the ionophores ensured excellent selectivity, the detection range could be adjusted with the ratio between the aqueous and the organic phases, and the divalent lucigenin ions also made the sensitivity for divalent target ions higher than conventional ionophore-based ion-selective optodes. The generalizability of the method was shown by changing the K+ ionophore into a Pb2+ ionophore, which successfully realized the highly selective detection of Pb2+ from 0.1 nM to 0.1 mM. Preliminary application of the method was demonstrated with the determination of K+ in diluted human blood serum (five samples), and the results agreed well with those obtained from potentiometric ion-selective electrodes.

Ionophore-Based Ion-Selective Nanosensors from Brush Block Copolymer Nanodots

“…On the one hand, new sensor signal transducers are being explored to replace the pH cross-responsive chromoionophore, resulting in sensing systems based on solvatochromism, − dye release, distance change, − and chemiluminescence . On the other hand, the ionophore-based nanosensing platform has taken in various functional materials, including quantum dots, poloxamers, conjugated polymers, organosilica, biodegradable polymers, and so on.…”

Enhanced chemiluminescence determination of paracetamol

“…5 The chemiluminescence (CL) analysis is a method for the determination of emitting radiation by the reaction of a CL reagent (the inhibitor, the activator) with the analyte. Therefore, measurement of the intensity of emitting radiation is employed for the analytical purpose 6 and has shown to be effective for the detection of PCM. 5,7 Several analytical methods have been presented for the measurement of PCM, such as: fluorimetry, 8 electrochemical, [9][10][11][12][13] spectrophotometry, [14][15][16] flow injection analysis (FIA), 17,18 chromatography, [19][20][21] and capillary electrophoresis.…”

Highly Sensitive Genosensing Coupling Rolling Circle Amplification with Multiple DNAzyme Cores for DNA Walking

“…DNAzymes are a class of catalytically active DNA sequences that are much more stable than common enzymes . The synthesis and modification of DNAzymes are convenient and cost-effective. , More importantly, the flexibility to design DNA sequences makes DNAzymes quite suitable for DNA machines-based biosensing . A DNA walker is a typical DNA machine in which stepwise walking motions of a specific strand (walker) along certain tracks could be triggered by target-initiated reactions. , The application of a DNAzyme as the trigger for the DNA walker is a good choice for a genosensor.…”

“…17 The synthesis and modification of DNAzymes are convenient and cost-effective. 18,19 More importantly, the flexibility to design DNA sequences makes DNAzymes quite suitable for DNA machines-based biosensing. 20 A DNA walker is a typical DNA machine in which stepwise walking motions of a specific strand (walker) along certain tracks could be triggered by targetinitiated reactions.…”