Chemiluminescent flow sensor for the determination of Paraoxon and Aldicarb pesticides

Roda, Aldo; Rauch, P.; Ferri, Elida Nora; Girotti, Stefano; Ghini, Severino; Carrea, Giacomo; Bovara, Roberto

doi:10.1016/0003-2670(94)85043-7

Cited by 57 publications

(17 citation statements)

References 17 publications

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“…This operational sequence was settled considering the reaction conditions pointed out. 15 The formed choline reacted with choline oxidase into the column C 2 generating hydrogen peroxide. Carbaryl inhibited the reaction between acetylcholine and acetylcholinesterase, thus causing a decrease in the generation of choline and hydrogen peroxide.…”

Section: Methodsmentioning

confidence: 99%

“…14,15 An overall comparison would be favorable to proposed procedure, presenting as advantage, a wider linear response range and higher throughput.…”

Section: Analytical Parametersmentioning

confidence: 99%

“…This requirement could be afforded by analytical procedures based on chemiluminescence. 14,15 In this context, an enzymatic method for the determination of pesticides using chemiluminescence detection could be proposed based on the reaction of acetylcholine with acetylcholinesterase (AchE) producing choline and acetic acid. Afterwards choline reacts with choline oxidize delivering hydrogen peroxide.…”

Section: Introductionmentioning

confidence: 99%

“…The reduction effect presents a directly relationship with pesticide concentration in the sample, thus analytical procedures employing chemiluminescence detection based on this feature have been developed. 14,15 Low reagent consumption is another parameter that should be considered because it affects favorably both the cost of analysis and decreases the waste generation. This requisite could be easily achieved by implementing the analytical procedure in a flow system based on multicommutation process, which afforded ability to handle small volumes of sample and reagent solutions.…”

Section: Introductionmentioning

confidence: 99%

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A chemiluminescence flow-based procedure for determination of carbaryl in natural waters exploiting multicommutation and enzymatic reaction

Pires¹,

Morales‐Rubio²,

Guárdia³

et al. 2007

J. Braz. Chem. Soc.

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Um procedimento empregando detecção quiluminescente para determinação de carbaril em águas naturais utilizando acetilcolinesterase e colina oxidase é descrito. O módulo de análise projetado para implementar o processo de multicomutação controlado por microcomputador foi constituído por cinco válvulas solenóides, duas colunas com enzimas imobilizadas em esferas de vidro e cela de fluxo para detecção de quimiluminescência. Nas melhores condições experimentais foi obtida faixa linear entre 25 a 700 μg L -1 de carbaril. Amostras de água foram fortificadas com carbaril e as recuperações variaram entre 95 e 101%. Limite de detecção e coeficiente de variação foram estimados em 11 μg L -1 (99,7% nível de confiança) e 1,3% (n = 20), respectivamente. Outras características analíticas tais como freqüência de amostragem de 72 determinações por hora, consumo de reagente de 2,1 mg de hexacianoferrato de potássio(III) e 0,069 mg de luminol por determinação, também, foram obtidas.A chemiluminescence procedure for the determination of carbaryl in natural waters using acetylcholinesterase and choline oxidase is described. The flow system designed to implement multicommutation approach controlled by microcomputer comprised five solenoid valves, two columns with immobilized enzymes on controlled pore glass beads and chemiluminometric flow cell. In the best experimental conditions a linear response ranging from 25 to 700 μg L -1 carbaryl was obtained. Water samples were spiked with carbaryl in order to access the accuracy and recoveries between 95 and 101% were obtained for a concentration level ranging from 25 to 100 μg L -1 carbaryl. Detection limit and variation coefficient were estimated as 11 μg L -1 (99.7% confidence level) and 1.3% (n = 20), respectively. Other profitable features such as a sample throughput of 72 determinations per hour, a reagent consumption of 2.1 mg potassium hexacyano ferrate(III) and 0.069 mg luminol per determination were also achieved.

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Section: Methodsmentioning

confidence: 99%

“…14,15 An overall comparison would be favorable to proposed procedure, presenting as advantage, a wider linear response range and higher throughput.…”

Section: Analytical Parametersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A chemiluminescence flow-based procedure for determination of carbaryl in natural waters exploiting multicommutation and enzymatic reaction

Pires¹,

Morales‐Rubio²,

Guárdia³

et al. 2007

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…Biosensing analytical devices, based on the acetylcholinesterase (AChE) inhibition test, using the monitoring of hydrogen peroxide generated as a result of the oxidation of choline produced from acetylcholine hydrolysis in the presence of choline oxidase have been reported for the quantification of carbamate and organophosphorus pesticides. In one of these approaches a column, placed inside a luminometer, was packed with cholineoxidase and peroxidase immobilized on methacrylate beads and AChE was added to the samples or immobilized in another column packed with the same solid support but placed outside the luminometer (Roda et al, 1994). The use of soluble AChE provided the best results when comparing with immobilized enzyme.…”

Section: Enzyme Inhibition-based Biosensorsmentioning

confidence: 99%