Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

Abstract

Multiplex technology is an important emerging field, in diagnostic sciences, that enables the simultaneous detection of several analytes in a single sample. The light-emission spectrum of a chemiluminescent phenoxy-dioxetane luminophore can...

Non-invasive diagnosis of bacterial and non-bacterial inflammations using a dual-enzyme-responsive fluorescent indicator

“…Accurately distinguishing between bacterial and non-bacterial inflammation holds significant clinical importance in diagnosing and treating inflammatory conditions. 16,17 Firstly, the prompt and accurate differentiation of inflammation is crucial in providing clinical guidance for patients' therapeutic strategies. Bacterial inflammation often necessitates the administration of antibiotics, whereas non-bacterial inflammation may require alternative interventions such as antifungal or antiviral medications.…”

Spirostrain-Accelerated Chemiexcitation of Dioxetanes Yields Unprecedented Detection Sensitivity in Chemiluminescence Bioassays

“…Several years ago, we discovered that the incorporation of an electron-withdrawing group (EWG) as a substituent at the ortho position of a phenoxy-adamantyl-1,2-dioxetane prevents water-mediated quenching of the excited intermediate and increases the light-emission intensity of the chemiluminescent luminophore by up to 3000-fold (Figure B) . This groundbreaking development enabled the use of our chemiluminescent luminophores as single-component probes with no required additives. − Numerous research groups worldwide, − including ours, took advantage of the ortho -substituted phenoxy-adamantyl-1,2-dioxetane luminophore to develop useful chemiluminescent probes for applications in cells and in vivo assays. − …”

“… 5 − 9 Numerous research groups worldwide, 10 − 16 including ours, took advantage of the ortho -substituted phenoxy-adamantyl-1,2-dioxetane luminophore to develop useful chemiluminescent probes for applications in cells and in vivo assays. 17 − 33 …”

High Contrast Bioimaging of Tumor and Inflammation with a Bicyclic Dioxetane Chemiluminescent Probe

“…The chemiluminescence (CL) method is increasingly recognized as a superior tool in biomedical research and diagnostics − due to its remarkable sensitivity, favorable signal-to-noise ratio, and independence from external light excitation, which altogether enhance the precision and clarity of biomedical imaging. − Despite its proven efficacy, the deployment of CL for the concurrent visualization of tumors and inflammatory sites through ONOO – detection using a single, bespoke probe has remained uncharted territory. This is largely attributable to the scarcity of ONOO – specific CL probes, their tendency to emit at shorter wavelengths, and their limited selectivity and depth of tissue penetration. − To overcome these challenges, our study introduces a novel NIR (near-infrared) CL probe tailored to detect peroxynitrite (ONOO – ) with unparalleled sensitivity and precision, paving the way for the targeted imaging of both inflammation and tumor tissues (Figure b).…”