Chemiluminescent detection of clonal immunoglobulin and T cell receptor gene rearrangements in Tunisian lymphoid malignancies, leukemias and lymphomas

Abed, Rym El; Khechine, Amel El; Omri, Halima El; Youssef, Sabri; Laatiri, Adnene; Lefranc, Marie‐Paule; Khélif, Abderrahim; Soua, Zohra

doi:10.1080/10428190600563250

Cited by 4 publications

(5 citation statements)

References 28 publications

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“…IMGT ® , http://www.imgt.org (accessed on 20 February 2022), the global reference in immunogenetics and immunoinformatics created by Marie-Paule Lefranc (LIGM, Université de Montpellier and CNRS), provides a unique scientific and computing frame for bridging loci, genes, alleles, sequences and structures of the IG or antibodies, TR and MH of the adaptive immune responses in humans and in other jawed vertebrates [167][168][169][170][171][172][173][174][175][176][177][178][179][180][181][182]. The IMGT standards are used for the description of the polymorphisms (genes, allotypes, alleles) [183][184][185][186][187][188][189][190], molecular mechanisms, genome evolution [191,192], susceptibility to diseases, autoimmunity, structure-functions, repertoire in infectious diseases [193][194][195][196][197][198], clonality and sequence analysis in leukemia and lymphoma [199][200][201][202][203][204][205][206], analysis of the content of the Fab and scFv combinatorial phage display libraries screened for identification of novel therapeutic antibody specificities…”

Section: Discussionmentioning

confidence: 99%

IMGT®Homo sapiens IG and TR Loci, Gene Order, CNV and Haplotypes: New Concepts as a Paradigm for Jawed Vertebrates Genome Assemblies

Lefranc

2022

Biomolecules

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IMGT®, the international ImMunoGeneTics information system®, created in 1989, by Marie-Paule Lefranc (Université de Montpellier and CNRS), marked the advent of immunoinformatics, a new science which emerged at the interface between immunogenetics and bioinformatics for the study of the adaptive immune responses. IMGT® is based on a standardized nomenclature of the immunoglobulin (IG) and T cell receptor (TR) genes and alleles from fish to humans and on the IMGT unique numbering for the variable (V) and constant (C) domains of the immunoglobulin superfamily (IgSF) of vertebrates and invertebrates, and for the groove (G) domain of the major histocompatibility (MH) and MH superfamily (MhSF) proteins. IMGT® comprises 7 databases, 17 tools and more than 25,000 pages of web resources for sequences, genes and structures, based on the IMGT Scientific chart rules generated from the IMGT-ONTOLOGY axioms and concepts. IMGT® reference directories are used for the analysis of the NGS high-throughput expressed IG and TR repertoires (natural, synthetic and/or bioengineered) and for bridging sequences, two-dimensional (2D) and three-dimensional (3D) structures. This manuscript focuses on the IMGT® Homo sapiens IG and TR loci, gene order, copy number variation (CNV) and haplotypes new concepts, as a paradigm for jawed vertebrates genome assemblies.

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Section: Discussionmentioning

confidence: 99%

IMGT®Homo sapiens IG and TR Loci, Gene Order, CNV and Haplotypes: New Concepts as a Paradigm for Jawed Vertebrates Genome Assemblies

Lefranc

2022

Biomolecules

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“…We have developed in our laboratory a chemiluminescent Southern blot assay to determine lymphoproliferation clonality using a set of immunoglobulin Ig probes including the Ig light chain Cκ one. 13 However, Cκ probe only deduces the deletional rearrangement of IGK gene whereas the more specific Kde probe allows the detection and confirmation of IGK deletion. Therefore, we have developed, cloned and used a specific Kde probe to precise clonality and diagnosis of B-ALL and B-CLL.…”

Section: Discussionmentioning

confidence: 99%

“…The hybridization signal was detected by CDP-Star chemiluminescent system (Amersham Pharmacia, Biotech) as previously described. 13 The IGK-Kde gene configuration was analyzed by mean of Alkalin phosphatase labeled IGK-Cκ or IGK-Kde probes.…”

Section: Southern Blot Analysismentioning

confidence: 99%

Molecular characterization of a Tunisian B-lineage leukemia using Kde gene rearrangements

Hamadou¹,

Besbes²,

Bouali³

et al. 2022

JCPCR

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Background: Molecular approaches applied to the study of B-lineage leukemia have made significant contributions in understanding and characterizing the disease. The Kde rearrangement was targeted as clonal marker to characterize Tunisian B-chronic lymphocytic leukemia (B-CLL) and B-acute lymphoblastic leukemia (B-ALL).Objective: We used a multiplex PCR to amplify deletional Kde rearrangements and Southern blot assay with Cκ and Kde probes in seven B-CLL and two B-ALL. Direct sequencing was performed in order to determine the n region.Results: An interesting RSS-Kde biallelic rearrangement was found in one adult male patient with B-ALL, since only Vκ-Kde rearrangement has been reported in adult B-ALL. In B-CLL, one case with two clonal cell proliferations in favor of oligoclonality which is very infrequent in B-CLL was identified and might be associated with bad outcome. These first results need more investigation to establish correlation between molecular characteristic and the poor outcome. Conclusion:The molecular approach was successfully improved and applied for clonality assessment of B-lineage leukemia for a better diagnosis and performing monitoring minimal residual disease.

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“…IMGT/V-QUEST is the reference tool for the clonality sequence analysis in leukemia and lymphoma [ 291 , 292 , 293 , 294 , 363 , 364 , 365 , 366 ], which has been extended to veterinary species owing to the availability and IMGT ® biocuration of the IG and TR loci [ 346 , 349 , 350 , 351 ], and the IMGT reference directories. Exploring hight-throughput repertoires with IMGT/HighV-QUEST provides standardized NGS analysis of IG and TR repertoires in experimental engineered (combinatorial libraries) or in physiological conditions (vaccination, immunodeficiency, autoimmune diseases, cancers and infectious diseases).…”

Section: Discussionmentioning

confidence: 99%

Immunoglobulins or Antibodies: IMGT® Bridging Genes, Structures and Functions

Lefranc¹,

Lefranc²

2020

Biomedicines

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IMGT®, the international ImMunoGeneTics® information system founded in 1989 by Marie-Paule Lefranc (Université de Montpellier and CNRS), marked the advent of immunoinformatics, a new science at the interface between immunogenetics and bioinformatics. For the first time, the immunoglobulin (IG) or antibody and T cell receptor (TR) genes were officially recognized as ‘genes’ as well as were conventional genes. This major breakthrough has allowed the entry, in genomic databases, of the IG and TR variable (V), diversity (D) and joining (J) genes and alleles of Homo sapiens and of other jawed vertebrate species, based on the CLASSIFICATION axiom. The second major breakthrough has been the IMGT unique numbering and the IMGT Collier de Perles for the V and constant (C) domains of the IG and TR and other proteins of the IG superfamily (IgSF), based on the NUMEROTATION axiom. IMGT-ONTOLOGY axioms and concepts bridge genes, sequences, structures and functions, between biological and computational spheres in the IMGT® system (Web resources, databases and tools). They provide the IMGT Scientific chart rules to identify, to describe and to analyse the IG complex molecular data, the huge diversity of repertoires, the genetic (alleles, allotypes, CNV) polymorphisms, the IG dual function (paratope/epitope, effector properties), the antibody humanization and engineering.

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Chemiluminescent detection of clonal immunoglobulin and T cell receptor gene rearrangements in Tunisian lymphoid malignancies, leukemias and lymphomas

Cited by 4 publications

References 28 publications

IMGT®Homo sapiens IG and TR Loci, Gene Order, CNV and Haplotypes: New Concepts as a Paradigm for Jawed Vertebrates Genome Assemblies

IMGT®Homo sapiens IG and TR Loci, Gene Order, CNV and Haplotypes: New Concepts as a Paradigm for Jawed Vertebrates Genome Assemblies

Molecular characterization of a Tunisian B-lineage leukemia using Kde gene rearrangements

Immunoglobulins or Antibodies: IMGT® Bridging Genes, Structures and Functions

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