Chemiluminescent detection of amino acids using in situ generated Ru(bpy)3+3

Electrochemiluminescent Systems as Devices and Sensors

“…167,247 In the in situ technique, analyte and Ru(bipy) 3 2 þ solutions are mixed before delivering them to the detection cell, which contains a working electrode. 17,[248][249][250] On the electrode surface, Ru(bipy) 3 2 þ is oxidized to Ru(bipy) 3 3 þ , which reacts with the analyte. This method is more convenient experimentally and better reproducible than the external generation technique.…”

Comparison Between Acridan Ester, Luminol, and Ruthenium Chelate Electrochemiluminescence

“…These assays are based on the reaction of electrochemically oxidized or reduced RuðbpyÞ 2þ 3 with various reductants or oxidants. A large number of analytes have been determined in this way, including oxalate [23], amino acids [24], nicotinamide cofactors [25] and glucose [26]. ECL has also been used to detect low concentrations of RuðbpyÞ 2þ 3 used as a label in immunoassays [7,27] and nucleotide assays [8,28].…”

Advances in the study of luminescence probes for proteins

“…The physiochemical properties of these complexes have been studied in detail [133,134]. There have been some reports dealing with the detection of amino acids and amines using tris(2,2 -bipyridyl) ruthenium(III) Ru(bpy) 3 3+ -based chemiluminescence [135][136][137]. Brune and Bobbitt [135] proposed a new technique for the detection of underivatized amino acids and amines using Ru(bpy) 3 3+ as a chemiluminescent reagent.…”