Chemiluminescent Analysis of Light-Irradiated Leukocytes as a Diagnostic Tool for Fast Identification of Pathological States

Sinyakov, Michael S.; Shlenskaya, Tanya S.; Belotsky, Sandro; Zhevelev, Helena M.; Shainberg, Bracha; Lubart, Rachel; Friedmann, H.; Avtalion, Ramy R.

doi:10.1089/pho.2006.2073

Cited by 3 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table 1 summarizes Pearson correlations computed for PMN counts and various BCL traits. In line with our previous report (1), no correlation existed between PMN counts and individual levels of BCL0, BCL2 and BCL5 (exemplified in Fig. 1A for BCL0).…”

Section: Resultssupporting

confidence: 93%

“…3D) were clearly different, the difference within DEL quartiles positioning being more pronounced. Even more remarkable distinction within DEL quartiles was evident after elimination of the light‐resistant points in the way we did in our previous work (1) from the data pool (Fig. 3E).…”

Section: Resultssupporting

confidence: 66%

“…Right column represents data for irradiation light intensities of 5 mW cm −2 . A = PMN quartiles; B = BCL quartiles; C = BCL0 quartiles; D = DEL quartiles; E = DEL quartiles after elimination of the light‐resistant points (1).…”

Section: Resultsmentioning

confidence: 99%

“…In our previous work (1), we used a group analysis of data based on PMN‐oriented approach and elimination of the light‐resistant points that prevented performance of this analysis. Accordingly, the results were interpreted on the group level.…”

Section: Discussionmentioning

confidence: 99%

“…We have recently suggested (1) that the mechanisms underlying PMN‐mediated ROS response to pathogen invasion and light irradiation are largely alike and include activation of PMN membrane‐associated NADPH‐oxidase complex and formation of hydrogen peroxide (HP). At the first stage, one electron transfer transforms molecular oxygen into superoxide anion (), which dismutates into HP and oxygen in the presence of protons.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Determinants of Pathology in Light‐irradiated Cells

Sinyakov¹,

Zhevelev²,

Avtalion³

2010

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

This work is devoted to further evaluation of pathological states in light-irradiated cells in terms of relationship between the level of reactive oxygen species (ROS) and polymorphonuclear neutrophil (PMN) count. Human leukocytes were isolated from 34 donors exhibiting flu-like symptoms, irradiated with a nonlaser blue light (2 and 5 mW cm(-2)) and examined for ROS production by a chemiluminescence (CL) assay. The level of ROS was measured as a basal chemiluminescence before (BCL0) and after (BCL) irradiation. In our preceding study (Sinyakov, M. et al. [2007] Photomed. Laser Surg.25, 257-263), PMN-oriented group analysis was applied to a data set divided into light-resistant and light-sensitive points, and cell responsiveness to light was suggested for prognostic evaluation of pathology. In the present work, BCL-oriented analysis was performed on the whole data set to evaluate the individual contribution of every point. BCL level and PMN count were confirmed to be independent variables. Sign-specific delta (DEL) values (DEL = BCL - BCL0) were assessed in relation to respective levels of BCL, BCL0 and PMN counts. DEL and BCL0 were found to be reliable photochemical indices of pathology. Taken together, the results obtained from group and individual analyses provide a novel outlook on the dynamics of pathological development and indicate a promising potential of the assay as a fast discriminator between the nonpathological state and different levels of pathology.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Determinants of Pathology in Light‐irradiated Cells

Sinyakov¹,

Zhevelev²,

Avtalion³

2010

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

show abstract

Vertical and horizontal transmission of tilapia larvae encephalitis virus: The bad and the ugly

et al. 2011

View full text Add to dashboard Cite

Impairment of innate immunity in tilapia larvae after vertical and horizontal infection with the newly characterized tilapia larvae encephalitis virus (TLEV) was accessed by evaluation of cell-mediated reactive oxygen species (ROS) production in affected fish with the use of horseradish peroxidase-amplified luminol-dependent chemiluminescence assay. The priming in-vivo infection with TLEV resulted in downregulation of ROS response in both vertically- and horizontally-infected fish; this suppression was further exacerbated by specific in-vitro booster infection with the same virus. Application of Ca ionophore and phorbol myristate acetate as alternative nonspecific boosters enabled restoration of ROS release in vertically-infected but not in horizontally-infected larvae. The results indicate severe TLEV-imposed phagocyte dysfunction in affected larvae. The difference in restoration potential of ROS production after vertical and horizontal virus transmission is interpreted in the frame of principal distinctions between the two modes.

show abstract

EFFECTS OF RED LIGHT AT 640 nm FROM LIGHT EMITTING DIODES ON THE RESPIRATORY BURST OF HUMAN NEUTROPHILS

Zhu

et al. 2008

J. Innov. Opt. Health Sci.

View full text Add to dashboard Cite

Photobiomodulation (PBM) has been reported to have effects on respiratory burst of polymorphonuclear neutrophils (PMNs), but little focus was on the individual differences of human PMNs. The latter was investigated in this study. The PMNs were isolated from peripheral blood of 13 volunteers (10 ordinary persons, 3 athletes) and treated by red light (640 ± 15 nm) from light emitting diodes (RLED) at 50, 100, 300, 500 and 1000 J/m2 for 100 seconds, respectively. Blood samples of athletes were extracted at different running stages in 10 km non-interrupted long-distance running, before running, 1 hour after running began, just finishing the running, resting for 1 hour and 2 hours after running. The PMN respiratory burst was assessed by the nitroblue tetrazolium test. It was found that there were three types of RLED PBM on the respiratory burst of 3 types of PMNs, respectively, inducing for the subactivated PMNs, inhibiting for the overactivated PMNs and none for the PMNs in homeostasis. It was then concluded that there may be RLED PBM on dysfunctional human PMNs while none on those in homeostasis, and RLED at 300 J/m2 for 100 seconds may have bi-direction modulation on PMN respiratory burst.

show abstract

Chemiluminescent Analysis of Light-Irradiated Leukocytes as a Diagnostic Tool for Fast Identification of Pathological States

Cited by 3 publications

References 33 publications

Determinants of Pathology in Light‐irradiated Cells

Determinants of Pathology in Light‐irradiated Cells

Vertical and horizontal transmission of tilapia larvae encephalitis virus: The bad and the ugly

EFFECTS OF RED LIGHT AT 640 nm FROM LIGHT EMITTING DIODES ON THE RESPIRATORY BURST OF HUMAN NEUTROPHILS

Contact Info

Product

Resources

About