Chemiluminescence of LUminol and Related Hydrazides: The Light Emission Step

White, Emil H.; Bursey, Maurice M.

doi:10.1021/ja01059a051

Cited by 229 publications

(128 citation statements)

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“…This instrument is based on the chemiluminescent reaction of 02 with luminol dianion in dimethyl sulfoxide (21). A stream of argon gas carries the 02, evolved by the cells, to the luminol solution; the light emitted in the reaction of 02 and luminol is measured by a photomultiplier.…”

Section: Methodsmentioning

confidence: 99%

The Development of Photosynthesis in a Greening Mutant of Chlorella and an Analysis of the Light Saturation Curve

Herron

Mauzerall

1972

Plant Physiol.

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Photosynthetic oxygen evolution considerably precedes the rise in chlorophyll during the greening of a yellow mutant of Chlorella vulgaris. Dark-grown cells required 20 times more light to saturate photosynthesis than light-grown or normal cells. The chlorophyll appears to add first to active reaction centers, then to fill in a more general antenna. The carotenoid pigments seem to add more randomly to the reaction centers.The shape of the light saturation curves can be explained with the assumption that an excitation in the antenna can reach several reaction centers. The efficiency of the total unit is constant during the greening process.The development of photosynthesis and of the structure of the chloroplast in greening plants has been studied extensively because of the unique opportunity to correlate structural development with photosynthetic function. The morphological changes on the scale above 10S of angstroms are well known (14,18), but the molecular details of this development of photosynthetic activity are less well understood. It is often thought that the development of photosynthesis parallels the biosynthesis of chlorophyll and the formation of grana lamellae. For example, during the greening of dark-grown Euglena, both photosynthesis and chlorophyll content are observed to rise together after a lag (2, 20). This rise coincides in time with the formation of grana. Greening of a mutant of Chlamydomonas (13,15) shows a somewhat faster increase of photosynthetic activity than of chlorophyll.We have studied the greening of a mutant of Chlorella (12), paying some attention to the quantitative aspects of measurements of photosynthetic activity. It is assumed that photosynthetic activity of a cell is proportional to the number of active reaction centers present in the cell. The rate of photosynthesis is in turn proportional to the number of active reaction centers under the condition of light saturation. We have found that much more light is required to saturate photosynthetic oxygen evolution in the early stages of greening than in the final fully greened cells. An analysis of these light saturation curves and of the in vivo absorption and fluorescence excitation spectra suggests a progressive addition of antenna chlorophyll to rapidly formed active reaction centers.

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Section: Methodsmentioning

confidence: 99%

The Development of Photosynthesis in a Greening Mutant of Chlorella and an Analysis of the Light Saturation Curve

Herron

Mauzerall

1972

Plant Physiol.

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“…The amphoteric nature of 1 determines its solubility. Therefore neutral 1 is soluble in organic solvents, whereas its alkaline salts 1c and 1d are soluble in water as in the case of luminol, 7,9 and the ratio between mono-and dianion (Scheme 1) is strongly dependent on the pH.…”

Section: Resultsmentioning

confidence: 99%

“…[1][2][3][4][5][6][7] In alkaline medium, in the presence of oxygen, [2][3][4]8 hydrogen peroxide, and transitional metal ions such as Fe 3+ , its CL intensity (I CL ) is enhanced. [9][10][11] Based on its CL, luminol is being used for the bioanalytical determination of enzymatically-formed oxidizing species such as the superoxide anion and the hydroxyl radical.…”

Section: Introductionmentioning

confidence: 99%