Chemiluminescence determination of catecholamines in human blood plasma using 1,2-bis(3-chlorophenyl)ethylenediamine as pre-column derivatizing reagent for liquid chromatography

Ragab, Gamal H.; Nohta, Hitoshi; Zaitsu, Kiyoshi

doi:10.1016/s0003-2670(99)00637-6

Cited by 71 publications

(32 citation statements)

References 28 publications

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“…Therefore, the determination of catecholamines in biological fluids is very important. Different methods, such as flow injection chemiluminescence (CL) detection [3,4], HPLC coupled with CL [5][6][7], fluorescence [8], and electrochemical (EC) [9,10] detection, have been used for the determination of catecholamines.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the CE-CL methods employ indirect CL detection. Hu et al [33] reported a CE/indirect CL procedure for the analysis of three catecholamines and catechol, based on their quenching effects on the CL reaction of luminol-K 3 [Fe(CN) 6 ] system. However, CE/direct CL detection for the catecholamines with a luminol-potassium periodate (KIO 4 ) system has not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

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Determination of catecholamines by CE with direct chemiluminescence detection

Liu

Wang

et al. 2007

Electrophoresis

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A rapid and sensitive method to detect three catecholamines, isoprenaline, epinephrine, and dopamine, by CE coupled with direct luminol-potassium periodate chemiluminescence (CL) detection is described. The conditions for CE separation and CL reaction were systematically optimized. Under the optimum conditions, the baseline separation of three catecholamines was achieved within 6.5 min. The LODs obtained in standard solution were 5.3 6 10 -8 mol/L for isoprenaline, 4.7 6 10 -8 mol/L for epinephrine, and 1.5 6 10 -7 mol/L for dopamine. The RSD of the migration time and peak area were less than 1.8 and 3.6% (n = 5), respectively. The present method was applied to the determination of the dopamine in urine samples of cigarette smokers and nonsmokers. The results obtained indicate that there is a close relationship between the content of dopamine in human urine and the amount of cigarettes smoked daily; the level of dopamine in smokers is higher than in nonsmokers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of catecholamines by CE with direct chemiluminescence detection

Liu

Wang

et al. 2007

Electrophoresis

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“…1. One peristaltic pump was used to deliver luminol and KIO 4 solutions at a flow rate of 2.7 mL/min. Another peristaltic pump was used to pump sample solution at a flow rate of 0.8 mL/min.…”

Section: Apparatusmentioning

confidence: 99%

“…A six-way injection valve with an 80 µL injector loop was used to inject sample solution. The streams of luminol, KIO 4 in alkaline medium and analyte were combined in a flow cell. The CL signal produced in the flow cell was detected with a photomultiplier tube and recorded by an IBM-compatible computer using flow-injection CL analysis system software (IFFL-DD, Xi'an Ruike Electronic Equipment Corporation, Xi'an, China).…”

Section: Apparatusmentioning

confidence: 99%

Flow‐injection chemiluminescence determination of catecholamines based on their enhancing effects on the luminol–potassium periodate system

et al. 2006

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A rapid and sensitive chemiluminescence (CL) method using flow injection analysis is described for the determination of four catecholamines, dopamine, adrenaline, isoprenaline and noradrenaline, based on their greatly enhancing effects on the CL reaction of luminol-potassium periodate in basic solutions. The optimized chemical conditions for the chemiluminescence reaction were 1.0 x 10(-4) mol/L luminol and 1.0 x 10(-5) mol/L potassium periodate in 0.2 mol/L sodium hydroxide (NaOH). Under the optimized conditions, the calibration graphs relating the CL signal intensity (peak height) to the concentration of the analytes were curvilinear and they were suitable for determining dopamine, adrenaline, isoprenaline, and noradrenaline in the range 0.1-10 ng/mL, 0.1-100 ng/mL, 1-100 ng/mL and 5-50 ng/mL, respectively, with the relative standard deviations of 0.8-1.7%. The detection limits of the method are 0.02 ng/mL for dopamine, 0.01 ng/mL for adrenaline, 0.1 ng/mL for isoprenaline and 2.0 ng/mL for noradrenaline. The sampling frequency was calculated to be about 60/h. The selectivity of the method was good, because a series of common ions or excipients, such as K(+), Ba(2+), CO(3)(2-), NO(3)(-), SO(4)(2-), PO(4)(3-), sodium citrate, sodium bisulphite, oxidate dopamine, starch, lactose, carbamide and gelatin, could not produce interference when their concentrations were 1000-fold than those of dopamine. The present method was successfully applied to the determination of the four catecholamines in pharmaceutical injections.

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“…A variety of techniques have been utilized for the determination of three catecholamines, such as chromatography, [1][2][3] capillary electrophoresis, [4][5][6] spectrophotometry, 7 fluorometry, [8][9][10] and electrochemical 11,12 detection. However, these methods are complicated (they need derivatization or combination with various means of detection).…”

Section: Introductionmentioning

confidence: 99%