Chemiluminescence and immunoassays

Rongen, H.A.H.; Hoetelmans, Richard M. W.; Bult, A.; Bennekom, W.P. van

doi:10.1016/0731-7085(94)80027-8

Cited by 113 publications

(54 citation statements)

References 109 publications

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“…The intensity of the emitting light was proportional to the amount of enzyme present and was inversely related to the amount of unlabelled T 3 in the sample. By reference to a series of T 3 standards assayed in the same way, the concentration of T 3 in the unknown sample was quantified (Rongen et al, 1994). The precise method for T 3 was repeated for T 4 .…”

Section: Physiological Parametersmentioning

confidence: 99%

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Iodine as an alleviator of bromine toxicity in thyroid, liver and kidney of broiler chickens

Toit

Casey

2012

Livestock Science

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The study pursued the hypothesis that bromine (Br) in drinking water at levels > 0.01 mg Br/L may have detrimental effects on the liver, kidneys and thyroid and the thyroid hormones T 3 and T 4 and that iodine (I) may alleviate the potential hazardous effect of Br. The research was done with mixed Ross broiler chickens over a 42-day post-hatch growth period. The trial design was six treatments, T1: 0 mg Br/L and 0 mg I/L; T2: 1 mg Br/L and 0 mg I/L; T3: 3 mg Br/L and 0 mg I/L; T4: 0 mg Br/L and 0.7 mg I/L; T5: 1 mg Br/L and 0.7 mg I/L; and T6: 3 mg Br/L and 0.7 mg I/L delivered via drinking water and three replicates per treatment with 30 birds per replicate. The effect of Br on T 3 and T 4 levels overall was nonsignificant, but T 3 and T 4 levels decreased between Weeks 4 and 6 with a significant effect at Week 6 on T 3 . Br had an overall effect on the thyroid gland (P = 0.0457), liver (P = 0.0025) and kidney (P = 0.0032), and had accumulated in these three organs. Histopathological assessment showed explicit damage to the livers that received the Br treatments. Iodine (0.07 mg/L) ameliorated the negative effects of high Br (3 mg/L Br) concentration and ingestion.

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Section: Physiological Parametersmentioning

confidence: 99%

“…In the T 3 CLIA, as explained by Rongen et al (1994), a certain amount of anti-T 3 antibody was coated on micro titer wells. A measured amount of chicken serum and a constant amount of T 3 conjugated with horseradish peroxidase were added to the micro titer wells.…”

Section: Physiological Parametersmentioning

confidence: 99%

Iodine as an alleviator of bromine toxicity in thyroid, liver and kidney of broiler chickens

Toit

Casey

2012

Livestock Science

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“…The TSH CLIA test utilises a unique monoclonal antibody directed against a distinct antigenic determinant on the intact TSH molecule [14]. The mean TSH values based on 160 random normal adult blood samples is 2.2 (0.27-4.2) μIU/ml.…”

Section: Tsh Analysesmentioning

confidence: 99%

The Efficacy of Thyrotropin Suppression Therapy in Treatment of Differentiated Thyroid Cancer after Total Thyroidectomy

Abo-Touk

Zayed

2015

Forum of Clinical Oncology

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Background: The aim of this prospective study was to assess the effect of the TSH suppression on both disease-free and overall survivals in patients with nonmetastatic differentiated thyroid cancer (DTC) after total thyroidectomy.Patients & Methods: One hundred and forty eight patients with pathologically proved operable differentiated thyroid carcinoma were enrolled in this prospective study. Levothyroxin (L-T4) therapy was started in doses according to treatment groups. Patients were randomly assigned to receive either postoperative TSH suppression therapy in group I (76 patients) or nonsuppression therapy in group II (72 patients).Results: During the period of follow up with a median 54 months, the disease-free survival for patients without TSH suppression therapy did not reach statistically significant difference comparing with those for patients with the suppression therapy (p=0.09). However, the difference was statistically significant for high-risk patients (p=0.04). On comparing both groups there was no statistically significant difference with regard to overall survival (p=0.17). The age of the patients more than 45 years, tumour size more than 4 cm and high-risk group were significant independent predictors for thyroid carcinoma-related relapse in univariate analysis. However, tumour size was the only significant factor in multivariate analysis.Conclusion: Suppressive treatment with L-T4 therapy in patients with differentiated thyroid carcinoma should be individualised and balanced against the adverse effects. TSH suppression is indicated in patients with high-risk disease or recurrent tumour. Normalisation of serum TSH is preferred for long-term treatment of disease-free elderly patients with DTC and comorbidities.

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“…6 Chemiluminescence (CL) has been exploited with a wide range of applications in different fields, such as biotechnology, pharmacology, molecular biology, and clinical and environmental chemistries. [7][8][9][10] However, only one CL method was reported for the determination of MP, which was based on reactions among tris-(2,2′-bipyridine)ruthenium(II), MP, hydrogen peroxide, Tween 20 and hydroxide ion. 11 In this method, the mixing order of the reagents considerably affects the CL signal.…”

Section: Introductionmentioning

confidence: 99%

Simple Chemiluminescence Method for the Determination of Mercaptopurine

Lau

Yagisawa

et al. 2002

ANAL. SCI.

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IntroductionMercaptopurine (MP) belongs to a general group of drugs known as antimetabolites, and is used to treat several types of cancers, including leukemia. MP interferes with the synthesis of nucleic acids, and thus disrupts the growth of cancer cells, which are then destroyed. However, because this drug can not distinguish cancer and normal cells, it causes side effects, since some normal cells are affected. Therefore, it is important to develop sensitive quantitative methods for controlling its administration.A number of spectrometric and fluorometric methods have already been successfully used for determining MP. For example, Tawa et al. developed a sensitive fluorometric method, based on the enzymatic oxidation of MP with xanthine oxidase to the oxypurine, followed by oxidation with acidic chromate to the corresponding fluorescent sulfonate. 1 Several liquid chromatographic methods have also been described. [2][3][4][5][6] Jonkers et al. determined MP in human plasma using HPLC, including post-column derivatization and fluorometric detection. 4 Van Os et al. measured MP using solid-phase extraction and reversed-phase HPLC. 5 Georget et al. determined MP in capsules prepared for paediatric patients by capillary zone electrophoresis. 6 Chemiluminescence (CL) has been exploited with a wide range of applications in different fields, such as biotechnology, pharmacology, molecular biology, and clinical and environmental chemistries.7-10 However, only one CL method was reported for the determination of MP, which was based on reactions among tris-(2,2′-bipyridine)ruthenium(II), MP, hydrogen peroxide, Tween 20 and hydroxide ion. 11 In this method, the mixing order of the reagents considerably affects the CL signal. Still, a simple and convenient method to measure mercaptopurine would be highly desirable.Here, we report on such a simple and convenient CL method for the determination of MP. We found that weak CL emitted after mixing H2O2 and luminol under alkaline conditions could be greatly enhanced by MP. Most importantly, this new reaction had a longer light signal, which was very convenient for routine applications, especially with a simple setup, since the mixing of CL reagents could generally be performed outside of the measuring device. Based on this fact, a simple and robust technique for the convenient measurement of MP was developed. The linear range was 1.5 mM -5 µM with a detection limit of 1 µM. Overall, the proposed CL method was simple, convenient and sufficiently sensitive for the determination of MP in the drug powder. Experimental ChemicalsA stock solution for 10 -2 M MP (Wako, Japan) was prepared by dissolving in 0.1 M NaOH. Other standard solutions were made by gradually diluting relative stock solutions with 0.1 M NaOH containing 2.5 mM EDTA. Hydrogen peroxide, luminol and other organic compounds were also purchased from Wako, Japan. An MP-containing drug, Leukerin, was obtained from Takeda Pharmaceutical Company, Japan. CL detection proceduresLight-producing reactions were carried out in 12 ×...

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Chemiluminescence and immunoassays

Cited by 113 publications

References 109 publications

Iodine as an alleviator of bromine toxicity in thyroid, liver and kidney of broiler chickens

Iodine as an alleviator of bromine toxicity in thyroid, liver and kidney of broiler chickens

The Efficacy of Thyrotropin Suppression Therapy in Treatment of Differentiated Thyroid Cancer after Total Thyroidectomy

Simple Chemiluminescence Method for the Determination of Mercaptopurine

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