Chemically modified nucleic acids and DNA intercalators as tools for nanoparticle assembly

Fazio, Angela Federica De; Misatziou, Doxi; Baker, Ysobel R; Muskens, Otto L.; Brown, Tom; Kanaras, Antonios G.

doi:10.1039/d1cs00632k

Cited by 20 publications

(20 citation statements)

References 280 publications

(383 reference statements)

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“…The photoreactive chemical group enhances the stability of DNA structures and DNA-AuNP crystals under DNA denaturing conditions by covalent crosslinking of DNA strands. [21][22][23][24][25] This feature is especially valuable for the encapsulation of proteins that are sensitive to environmental conditions, including the pH and ionic concentration.…”

Section: Resultsmentioning

confidence: 99%

DNA-functionalized colloidal crystals for macromolecular encapsulation

et al. 2022

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Section: Resultsmentioning

confidence: 99%

DNA-functionalized colloidal crystals for macromolecular encapsulation

et al. 2022

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“…Nucleic acid chemical modification technologies enriched the structure and function of DNA molecules and expanded the application of DNA materials in bioanalysis. 55 On the basis of the modification technologies, more kinds of nanoparticles have been introduced via DNA supramolecular assembly on nanoscale interfaces. For example, the modification of sulfhydryl group allows DNA to assemble on the interfaces of gold nanoparticles (AuNPs) with high efficiency through thiolate−Au bonds.…”

Section: Modified Dna On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

“…Nucleic acid chemical modification technologies enriched the structure and function of DNA molecules and expanded the application of DNA materials in bioanalysis . On the basis of the modification technologies, more kinds of nanoparticles have been introduced via DNA supramolecular assembly on nanoscale interfaces.…”

Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

Yao

Tang

et al. 2022

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Facing increasing demand for precision medicine, materials chemistry systems for bioanalysis with accurate molecular design, controllable structure, and adjustable biological activity are required. As a genetic biomacromolecule, deoxyribonucleic acid (DNA) is created via precise, efficient, and mild processes in life systems and can in turn precisely regulate life activities. From the perspective of materials chemistry, DNA possesses the characteristics of sequence programmability and can be endowed with customized functions by the rational design of sequences. In recent years, DNA has been considered to be a potential biomaterial for analysis and has been applied in the fields of bioseparation, biosensing, and detection imaging. To further improve the precision of bioanalysis, the supramolecular assembly of DNA on micro/nanointerfaces is an effective strategy to concentrate functional DNA modules, and thus the functions of DNA molecules for bioanalysis can be enriched and enhanced. Moreover, the new modes of DNA supramolecular assembly on micro/nanointerfaces enable the integration of DNA with the introduced components, breaking the restriction of limited functions of DNA materials and achieving more precise regulation and manipulation in bioanalysis. In this Account, we summarize our recent work on DNA supramolecular assembly on micro/nanointerfaces for bioanalysis from two main aspects. In the first part, we describe DNA supramolecular assembly on the interfaces of microscale living cells. The synthesis strategy of DNA is based on rolling-circle amplification (RCA), which generates ultralong DNA strands according to circular DNA templates. The templates can be designed with complementary sequences of functional modules such as aptamers, which allow DNA to specifically bind with cellular interfaces and achieve efficient cell separation. In the second part, we describe DNA supramolecular assembly on the interfaces of nanoscale particles. DNA sequences are designed with functional modules such as targeting, drug loading, and gene expression and then are assembled on interfaces of particles including upconversion nanoparticles (UCNPs), gold nanoparticles (AuNPs), and magnetic nanoparticle (MNPs). The integration of DNA with these functional particles achieves cell manipulation, targeted tumor imaging, and cellular regulation. The processes of interfacial assembly are well controlled, and the functions of the obtained bioanalytical materials can be flexibly regulated. We envision that the work on DNA supramolecular assembly on micro/nanointerfaces will be a typical paradigm for the construction of more bioanalytical materials, which we hope will facilitate the development of precision medicine.

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“…LNAs have a very high affinity and excellent specificity for complementary DNA and RNA, and LNA oligonucleotides have been used as antisense molecules in vitro and in vivo. The therapeutic effect of LNA gene silencing has been verified in many experiments . Tassone et al designed an inhibitor that uses locked nucleic acid (LNA) technology to increase affinity to miR-221 and resistance to nucleases.…”

Section: Mbs: Structure Classification and Their Applications In Rna ...mentioning

confidence: 99%

“…The therapeutic effect of LNA gene silencing has been verified in many experiments. 64 Tassone et al designed an inhibitor that uses locked nucleic acid (LNA) technology to increase affinity to miR-221 and resistance to nucleases. LNA-miR-221 inhibitors show good antitumor effects in multiple myeloma cell (MM) xenograft models, which supports LNA-miR-221 inhibitors as a promising anti-MM drug.…”

Section: Mbs: Structure Classification and Their Applications In Rna ...mentioning

confidence: 99%

Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging

Yang

Song

Wang

et al. 2022

ACS Appl. Nano Mater.

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As one of the two nucleic acids used by extant biochemistry, RNA is of great importance for cellular function and development, and its expression can affect cell homeostasis. Therefore, accurate detection of RNA is the key contributor to disease diagnosis, including tumor detection. Compared with conventional RNA detection methods, molecular beacons (MBs) have high selectivity for target molecules detection, which have been widely used in RNA detection. However, the background fluorescence of typical MBs will seriously affect the detection sensitivity and accuracy. In addition, the poor affinity and weak resistance to the nuclease digestion of MBs also limit its applications. With the development of nanotechnology, many nanomaterials with fluorescence quenching ability have been widely used as quenchers and carriers for MBs to construct nanoprobes (Nano-MBs). In this review, we summarize the recent developments of Nano-MBs in RNA detection and imaging over the past decade. First, the structure, working principle, and classification of MBs are discussed. Then, we introduce several typical Nano-MBs based on gold nanoparticles, polydopamine, graphene oxide, molybdenum disulfide, and quantum dots. Particular emphasis is placed on the application of Nano-MBs in mRNA and miRNA detection and imaging in the diagnosis and treatment of diseases, as well as in cancer. Finally, the current challenges and future perspectives are also discussed.

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Chemically modified nucleic acids and DNA intercalators as tools for nanoparticle assembly

Abstract: The chemical manipulation of DNA offers new tools to tune the properties of nanoparticle self assemblies.

Cited by 20 publications

References 280 publications

DNA-functionalized colloidal crystals for macromolecular encapsulation

DNA-functionalized colloidal crystals for macromolecular encapsulation

DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging

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