Chemically induced common bean (Phaseolus vulgaris L.) sprouts ameliorate dyslipidemia by lipid intestinal absorption inhibition

Mendoza-Sánchez, Magdalena; Pérez‐Ramírez, Iza F.; Wall‐Medrano, Abraham; Martinez-Gonzalez, Alejandra I.; Gallegos‐Corona, Marco A.; Reynoso‐Camacho, Rosalía

doi:10.1016/j.jff.2018.10.032

Cited by 16 publications

(15 citation statements)

References 37 publications

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“…Quercetin‐3‐ O ‐rutinoside (peak 4), kaempferol‐3‐ O ‐rutinoside (peak 7), (+)‐catechin (peak 11), and kaempferol‐3‐ O ‐glucoside (peak 15) were positively identified according to their retention, mass, and UV–vis characteristics by comparison with commercial standards. To the best of our knowledge, catechin seems to be one of the most common phenolic compounds found in the seeds of P. vulgaris Peak 4 (quercetin‐3‐ O ‐rutinoside) and peak 15 (kaempferol‐3‐ O ‐glucoside) have been previously identified in 80% (v/v) aqueous acetone extracts obtained from P. vulgaris L., whereas peak 7 (kaempferol‐3‐ O ‐rutinoside) was also found in the hydromethanolic extract prepared from pods of P. vulgaris obtained from Almeria, Spain …”

Section: Resultsmentioning

confidence: 90%

“…Quercetin-3-O-rutinoside (peak 4), kaempferol-3-O-rutinoside (peak 7), (+)-catechin (peak 11), and kaempferol-3-O-glucoside (peak 15) were positively identified according to their retention, mass, and UV-vis characteristics by comparison with commercial standards. To the best of our knowledge, catechin seems to be one of the most common phenolic compounds found in the seeds of P. vulgaris 27 Peak 4 (quercetin-3-O-rutinoside) and peak 15 (kaempferol-3-O-glucoside) have been previously identified in 80% (v/v) aqueous acetone extracts obtained from P. vulgaris L., 28 whereas peak 7 (kaempferol-3-O-rutinoside) was also found in the hydromethanolic extract prepared from pods of P. vulgaris obtained from Almeria, Spain. 29 Peaks 29 Berger et al 30 Flavan-3-ols were also detected, corresponding to peaks 11 and 13, which showed UV spectra with max 279-280 nm, characteristic of these compounds; thus, peak 13 presented a pseudomolecular ion ([M − H] − at m/z 577) corresponding to procyanidin dimers.…”

Section: Comparative Analysis Of Phenolic Content In Each Seed and Pomentioning

confidence: 96%

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Bioactive properties of greenhouse‐cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water‐stress effect

et al. 2019

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BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water‐stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water‐stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water‐stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 90%

Section: Comparative Analysis Of Phenolic Content In Each Seed and Pomentioning

confidence: 96%

Bioactive properties of greenhouse‐cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water‐stress effect

et al. 2019

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“…The modern lifestyle with little body activity and the excess of triglycerides and free fatty acids from the diet could lead to obesity and dyslipidemia [ 32 ]. Among commercially available obesity treatments, one of the most used is that directed to inhibit PL.…”

Section: Discussionmentioning

confidence: 99%

“…The virtual exploration based on the molecular docking by the affinity of molecular targets related to obesity is currently booming, specifically with PL inhibitors [ 32 , 35 , 36 ]. The results of this work show that after in silico hydrolysis of CP albumin and vicilin, it was possible to generate peptides with potential affinity to interact with human PL (PDB: 1LPB).…”

Section: Discussionmentioning

confidence: 99%

Cocoa (Theobroma cacao L.) Seed Proteins’ Anti-Obesity Potential through Lipase Inhibition Using In Silico, In Vitro and In Vivo Models

Coronado-Cáceres

Rabadán-Chávez

Mojica

et al. 2020

Foods

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The aim of this study was to determine the pancreatic lipase (PL) inhibitory effect of cocoa protein (CP) hydrolysates (CPH) using in silico and in vitro approaches, and an in vivo high-fat diet (HF) obese rat model. The results showed better theoretical affinity on PL for cocoa peptides EEQR, GGER, QTGVQ, and VSTDVNIE released from vicilin and albumins (−6.5, −6.3, −6.2, and −6.1 kcal/mol, respectively). Absorption, distribution, metabolism, and excretion (ADMET) prediction showed the human intestinal absorption (HIA) capacity of orlistat and eight cocoa peptides, demonstrating that they presented a low probability of toxicity with values lower than 0.6, while the orlistat has a high probability of hepatotoxicity with a mean value of 0.9. CPH (degree of hydrolysis of 55%) inhibited PL with an IC50 (concentration needed to inhibit 50% of enzyme activity) value of 1.38 mg/mL. The intragastric administration of 150 mg CP/kg/day to rats increased total lipids and triglycerides excretion in feces, ranging from 11% to 15% compared to the HF-diet. The HF + CP-diet also significantly decreased (p < 0.05) the apparent rate of fat absorption compared with the HF group. These results suggest that CP has anti-obesity potential by inhibiting PL, thus helping to prevent the development of non-communicable diseases.

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“…Formalin‐fixed samples (liver and WAT) were embedded in paraffin, sliced (4–5 μm thick), deparaffinized, rehydrated, and stained with hematoxylin and eosin following the procedure reported by Mendoza‐Sánchez et al () with some adaptations. Double‐blind observations were performed by two trained pathologists, using a fluorescent microscope (LEICA DM2000, Leica Microsystems Inc., Chicago, IL, USA).…”

Section: Methodsmentioning

confidence: 99%

Fiber and phenolic compounds contribution to the hepatoprotective effects of mango diets in rats fed high cholesterol/sodium cholate

Canizales

Domínguez-Ávila

Wall‐Medrano

et al. 2019

Phytotherapy Research

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The present study evaluated the contribution of mango fiber (MF) and mango phenolic compounds (MP) to the hepatoprotective effect of freeze‐dried mango pulp (FDM) cultivar (cv.) “Ataulfo” diets in high cholesterol/sodium cholate (HCC)–fed rats. Male Wistar rats were fed with a HCC diet for 12 weeks, either untreated, or supplemented with MF, MP, FDM, or a control diet (no HCC; n = 6/group). All mango treatments significantly decreased hepatic cholesterol deposition and altered its fatty acid profile, whereas MF and MP mitigated adipose tissue hypertrophy. MF caused a lower level of proinflammatory cytokines (IL‐1α/β, IFN‐γ, TNF‐α) whereas FDM increased the anti‐inflammatory ones (IL‐4, 6, 10). Mango treatments increased catalase (CAT) activity and its mRNA expression; superoxide dismutase (SOD) activity was normalized by MF and FDM, but its activity was unrelated to its hepatic mRNA expression. Changes in CAT and SOD mRNA expression were unrelated to altered Nrf2 mRNA expression. Higher hepatic PPARα and LXRα mRNA levels were found in MP and MF. We concluded that MF and MP are highly bioactive, according to the documented hepatoprotection in HCC‐fed rats; their mechanism of action appears to be related to modulating cholesterol and fatty acid metabolism as well as to stimulating the endogenous antioxidant system.

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Chemically induced common bean (Phaseolus vulgaris L.) sprouts ameliorate dyslipidemia by lipid intestinal absorption inhibition

Cited by 16 publications

References 37 publications

Bioactive properties of greenhouse‐cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water‐stress effect

Bioactive properties of greenhouse‐cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water‐stress effect

Cocoa (Theobroma cacao L.) Seed Proteins’ Anti-Obesity Potential through Lipase Inhibition Using In Silico, In Vitro and In Vivo Models

Fiber and phenolic compounds contribution to the hepatoprotective effects of mango diets in rats fed high cholesterol/sodium cholate

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