Chemically Induced Breast Tumors in Rats Are Detectable in Early Stages by Contrast Enhanced Magnetic Resonance Imaging but Not by Changes in the Acute-Phase Reactants in Serum

Abstract

The present study was undertaken to develop a rat model for monitoring the early development of breast cancer. Twelve female rats were divided into two groups of six rats that were either treated with N-methyl-N-nitrosourea to induce breast cancer or with bacterial lipopolysaccharide to induce inflammation. Serum samples taken from the rats prior to the treatment were used as controls. By the 14th week, presence of the tumor was detectable by contrast enhanced magnetic resonance imaging and confirmed by histopathology. When the serum proteins of the rats were examined by 2-dimensional electrophoresis (2-DE), no difference could be detected in the profiles of all proteins before and 18 weeks after administration of N-methyl-N-nitrosourea. However, higher expression of alpha-1B glycoprotein was detectable by 2-DE in serum samples of rats at the 18th week post-treatment with lipopolysaccharide.

Biochemical and Antioxidant effect of Doxorubicin hydrochloride and Propolis on N-methyl-N-nitrosourea (MNU) Induced Adenocarcinoma in Rats

“…Seventy days post-treatment, the mammary glands showed low grade, sclerosing ductal carcinomas and moderated hyperplasia in the lymphocytes of lymphoid follicles. Our results agreed partially with (Golbabapour et al, 2011).…”

<p>A multicenter study of a contrast-enhanced ultrasound diagnostic classification of breast lesions</p>

“…As highlighted by Zhang et al,34 subjectivity plays a major role in determining whether there is heterogeneous enhancement of small lesions, and it may lead to high-false negative rates. For malignant lesions, with the enlargement of the lesion size, vessel heterogeneity is more prominent, spatial distribution is unbalanced, terminal fine vessels are increased, and tumor vessel branches are irregular 35. Nevertheless, taken together, these results suggest that large lesions should be at least considered suspicious, even in the presence of negative CEUS diagnosis.…”

“…For malignant lesions, with the enlargement of the lesion size, vessel heterogeneity is more prominent, spatial distribution is unbalanced, terminal fine vessels are increased, and tumor vessel branches are irregular. 35 Nevertheless, taken together, these results suggest that large lesions should be at least considered suspicious, even in the presence of negative CEUS diagnosis.…”

Evaluation of the anticancer properties of the predicted hBaxBH3-mimetic compound 2-hydroxy-3,5-dinitrobenzamide in a mammary carcinogenesis-induced rat model

“…4B) and the observations are in line with the literature reports on the NMU-induced cancerous animal models. 35,36 Interestingly, 20%, 80% and 50% of rats belonging to Group IV (low-dose group), Group V (medium-dose group) and Group VI (high-dose group), respectively showed breast tissues as similar as to that of control group. It is also worthy of mentioning that the rest of 80% rats in Group IV were characterized to have mild dysplasia in their breast tissues, while the rest of 20% and 50% of rats in Group V and Group VI depicted basophilia in alveolar and ductal epithelium.…”