Biochemical and Antioxidant effect of Doxorubicin hydrochloride and Propolis on N-methyl-N-nitrosourea (MNU) Induced Adenocarcinoma in Rats

Badr, Mohamed; Neamat‐Allah, Ahmed N. F.; Abdallah, Amany A. M.; Hashem, Mohamed A.; Edrees, N.; El-Deen, Nasr A.M. Nasr; Ismail, Hager Tarek H.

doi:10.15835/buasvmcn-vm:11208

Cited by 3 publications

(2 citation statements)

References 28 publications

(33 reference statements)

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“…kidney αSMA αSMA immunopositive expression was proved among all groups, whether in the control, untreated EACbearing mice or treated EAC-bearing mice with SM, KBrO 3 and a combination of SM and KBrO 3 (Figs. [35][36][37][38][39]. Control group of mice showed very mild positive brown expression in interstitial tissue (Fig.…”

Section: Electron Microscopic Studiesmentioning

confidence: 99%

Silymarin and Potassium Bromate in EAC-bearing Mice: histopathological and Immunohistological study

Elhadad

Mahfouz

Ghanem

et al. 2022

Preprint

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Cancer is one of the leading causes of death worldwide. Reactive oxygen species induce pathology by damaging lipids, proteins, and DNA. Elevated reactive oxygen species levels, accompanied with down-regulation of cellular antioxidant enzyme systems, result in malignant transformation. Potassium bromate is a chemical oxidizing agent that is a famous flour improver. It can attack cellular constituents through reactive oxygen species and destroy the cellular structures so it may lead to cancer as a result of administration of it for a long time. Plant polyphenols such as silymarin reported to be a safe nontoxic agent to treat several diseases such as cancer. The anticancer mechanism of action of silymarin has been attributed to its antioxidant activity, antiproliferation, and cell cycle arrest, as it causes apoptosis in cancer cells. We aimed to investigate the effect of silymarin, and administration of potassium bromate on mice with Ehrlich ascites carcinoma cells. Histopathological, ultrastructural and immunohistochemical studies were applied on the liver, kidney, and Ehrlich ascites carcinoma cells. Silymarin was found to destroy tumors and improve the histological changes induced by Ehrlich ascites carcinoma cells in liver and kidney of Ehrlich ascites carcinoma-bearing mice. Potassium bromate showed an improvement in the histological changes induced by Ehrlich ascites carcinoma cells in liver and kidney of Ehrlich ascites carcinoma-bearing mice and caused mitochondrial degeneration and nuclear distortion in Ehrlich ascites carcinoma cells but showed no effect on non Ehrlich ascites carcinoma-bearing mice. The present study revealed that silymarin and potassium bromate could be very promising anticancer agents.

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Section: Electron Microscopic Studiesmentioning

confidence: 99%

Silymarin and Potassium Bromate in EAC-bearing Mice: histopathological and Immunohistological study

Elhadad

Mahfouz

Ghanem

et al. 2022

Preprint

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“…Oxidative Medicine and Cellular Longevity albumins concentration, and an increase of globulins. Hypoalbuminemia observed in MNU-exposed group can be a consequence of the injurious effects of the MNU carcinogen on the hepatic polyribosomes and protein-soluble factors, and the result of alkylation and carboxylation of RNA and proteins [43]. However, the negligible reduction of total blood proteins can be a consequence of proteolysis.…”

mentioning

confidence: 99%

Chemopreventive Effects of Propolis in the MNU-Induced Rat Mammary Tumor Model

Gal

Stan

Tăbăran

et al. 2020

Oxidative Medicine and Cellular Longevity

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Currently, one of the central problems in cancer management is the relapse of disease following conventional treatments, yet few therapeutic agents targeting resistance and tolerance exist. Propolis is known as a healing agent since ancient times. Therefore, over time, its curative properties have kept the interest of scientists, thus leading permanently to investigations of its other possible undiscovered effects. In this context, current experiments were performed to establish the chemopreventive potential of propolis extract (PE) (1.05 mg/kg BW/day) in N-methyl-N-nitrosourea- (MNU-) induced rat mammary tumors. MNU-inoculated/PE-treated rats had tumors of different physical attributes compared with control rats MNU-inoculated. The number of developed tumors (mean 49% versus 100%), incidence (mean 49% versus 100%), multiplicity (1.8 versus 3.7 (p<0.001)), tumor volume (mean 10 cm3 versus 16 cm3 (p<0.001)), and weight of the tumor mass (mean 7.42 g versus 9.00 g (p<0.05)) were noted. The numbers of grade I tumors recorded for MNU-inoculated rats were 24 (Group 1) and 7 (Group 2) for MNU-induced/PE-treated rats. In the serum of rats MNU-inoculated/PE-treated were found higher levels of antioxidative enzymes (SOD, CAT, and GPx) than in MNU-induced. Taken together, these data indicate that propolis could be a chemopreventive agent against MNU-induced mammary carcinogenesis.

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An Overview of the Evidence and Mechanism of Drug–Herb Interactions Between Propolis and Pharmaceutical Drugs

et al. 2022

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With the growing interest in the medicinal use of propolis, numerous studies have reported significant interactions between propolis extract and pharmaceutical drugs which may result in great clinical benefits or risks. The present study aims to review the drug–herb interactions of the full-spectrum propolis extract and main pharmaceutical drugs from the pharmacodynamic and pharmacokinetic aspects and elucidate the underlying pharmacological mechanisms. A literature search was conducted between June 2021 and February 2022 in Google Scholar, PubMed, MEDLINE, and EMBASE databases to include English studies from years 2000 to 2022 that evaluated the interaction of full-spectrum propolis extract and standard pharmaceutical drugs/cytochromes P450s. Studies that looked into geopropolis, propolis fractions, and isolated compounds, or interaction of propolis with foods, bioactive molecules, or receptors other than standard pharmaceutical drugs were excluded. From a pharmacodynamic perspective, propolis extract exhibited positive or synergistic interaction with several chemotherapeutic drugs by enhancing antitumor activity, sensitizing the chemoresistance cell lines, and attenuating multi-organ toxicity. The molecular mechanisms were associated with upregulating the apoptotic signal and immunomodulatory activity and attenuating oxidative damage. Propolis extract also enhanced the anti-bacterial and antifungal activities of many antimicrobial drugs against sensitive and resistant organisms, with an effect against the gram-positive bacteria stronger than that of the gram-negative bacteria. The synergistic action was related to strengthened action on interfering cell wall integrity and protein synthesis. The strong antioxidant activity of propolis also strengthened the therapeutic effect of metformin in attenuating hyperglycemia and pancreatic damage, as well as mitigating oxidative stress in the liver, kidney, and testis. In addition, propolis showed a potential capacity to enhance short-term and long-term memory function together with donepezil and improve motor function with levodopa and parasite killing activity with praziquantel. Pharmacokinetic studies showed inhibitory activities of propolis extracts on several CYP450 enzymes in vitro and in vivo. However, the effects on those CYP450 were deemed insignificant in humans, which may be attributed to the low bioavailability of the contributing bioactive compounds when administered in the body. The enhanced bioactivities of propolis and main pharmaceutical drugs support using propolis in integrative medicine in anti-cancer, anti-microbial, antidiabetic, and neurological disorders, with a low risk of altered pharmacokinetic activities.

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Biochemical and Antioxidant effect of Doxorubicin hydrochloride and Propolis on N-methyl-N-nitrosourea (MNU) Induced Adenocarcinoma in Rats

Cited by 3 publications

References 28 publications

Silymarin and Potassium Bromate in EAC-bearing Mice: histopathological and Immunohistological study

Silymarin and Potassium Bromate in EAC-bearing Mice: histopathological and Immunohistological study

Chemopreventive Effects of Propolis in the MNU-Induced Rat Mammary Tumor Model

An Overview of the Evidence and Mechanism of Drug–Herb Interactions Between Propolis and Pharmaceutical Drugs

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