Chemical Unity and Diversity in Bacterial Catabolism

Dagley, S.

doi:10.1007/978-1-4613-0803-4_4

Cited by 6 publications

(4 citation statements)

References 62 publications

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“…Organic carbon is an essential source of energy for all heterotrophic (micro)organisms. To obtain energy, microorganisms can use, through various catabolic pathways, numerous organic compounds, ranging from simple carbohydrates or organic acids to complex polymers 1 , 2 . The available carbon source and genetic determinants of the microorganisms define the metabolic pathways employed in a given environment.…”

Section: Introductionmentioning

confidence: 99%

The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Matuszewska

Maciag

Rajewska

et al. 2021

Sci Rep

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Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

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Section: Introductionmentioning

confidence: 99%

The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Matuszewska

Maciag

Rajewska

et al. 2021

Sci Rep

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show abstract

“…Organic carbon is an essential source of energy for all heterotrophic (micro)organisms. To obtain energy, microorganisms can use, through various catabolic pathways, numerous organic compounds, ranging from simple carbohydrates or organic acids to complex polymers [1], [2]. The available carbon source and genetic determinants of the microorganisms define the metabolic pathways to be employed in a given environment.…”

Section: Introductionmentioning

confidence: 99%

Necessity requires no decision: carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Matuszewska

Maciag

Rajewska

et al. 2021

Preprint

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Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7‑hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

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“…In an excellent chapter by Dagley (1989), the importance of bacterial metabolic diversity has been examined. The remarkable catabolic diversity of the microbial world is an integral part of the global carbon cycle.…”

Section: Introductionmentioning

confidence: 99%

“…Micro-organisms are capable of metabolizing every type of natural organic compound and a multitude of synthetic compounds. As summarized by Dagley (1989), micro-organisms accomplish this by using a narrow range of reaction mechanisms not much different from the mechanisms used to degrade universal biochemicals. However, it is necessary to note that in natural communities of diverse micro-organisms, the complete metabolism of some compounds may require several species of micro-organisms (Dagley 1989) that may or may not be taxonomically closely related.…”

Section: Introductionmentioning

confidence: 99%

Microbial diversity in soil: effect of releasing genetically engineered micro‐organisms

et al. 1994

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This review examines the potential for change in microbial diversity, with the emphasis on bacteria, in soil resulting from the introduction of genetically engineered microorganisms (GEMs). With the advent of GEMs came the impetus for new technologies to recover these micro‐organisms from soil and to assess their effects on microbial diversity. This review also presents general aspects of and genetic approaches to accessing bacterial diversity in the environment.

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Chemical Unity and Diversity in Bacterial Catabolism

Cited by 6 publications

References 62 publications

The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Necessity requires no decision: carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Microbial diversity in soil: effect of releasing genetically engineered micro‐organisms

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