Microbial diversity in soil: effect of releasing genetically engineered micro‐organisms

Leung, Ka Yin; England, Laura S.; Cassidy, Mike; Trevors, J. T.; Weir, Susan C.

doi:10.1111/j.1365-294x.1994.tb00081.x

Cited by 24 publications

(11 citation statements)

References 109 publications

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“…Indeed, fields located at Pieve d'Olmi and Dragoni can be characterized as high‐input systems, because of the heavy use of pesticides, herbicides and fertilizers, whereas the field at S. Maria di Galeria, being an experimental field, is characterized as a low‐input system where only manure is used as fertilizer, and no pesticides or herbicides are applied. Moreover, it is well known that management practices influence microbial activities in long‐term agricultural lands (Bolton et al ., 1985; Ramsay et al ., 1986), and that diversity is generally lower in microbial communities under stress in agroecosystems (Atlas et al ., 1991; Leung et al ., 1994). Therefore, we hypothesize that the management practices applied in the fields located at Pieve d'Olmi and Dragoni may induce stress on the microbial communities and, as a consequence, reduce genomovar diversity of the B. cepacia complex in these two fields.…”

Section: Discussionmentioning

confidence: 99%

Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy

Fiore¹,

Laevens²,

Bevivino³

et al. 2001

Environmental Microbiology

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Burkholderia cepacia is a 'complex' in which seven genomic species or genomovars have so far been identified. It appears that all seven B. cepacia genomovars are capable of causing infections in vulnerable persons; in particular, the importance of Burkholderia multivorans (genomovar II) and B. cepacia genomovar III among cystic fibrosis isolates, especially epidemic ones, has been emphasized. In order to acquire a better comprehension of the genomovar composition of environmental populations of B. cepacia, 120 strains were isolated from the rhizosphere of maize plants cultivated in fields located in northern, central and southern Italy. The identification of the different genomovars was accomplished by a combination of molecular polymerase chain reaction (PCR)-based techniques, such as restriction fragment length polymorphism (RFLP) analysis of 16S rDNA (ARDRA), genomovar-specific PCR tests and RFLP analyses based on polymorphisms in the recA gene whole-cell protein electrophoresis. ARDRA analysis allowed us to distinguish between all B. cepacia genomovars except B. cepacia genomovar I, B. cepacia genomovar III and Burkholderia ambifaria (genomovar VII). The latter genomovars were differentiated by means of recA PCR tests and RFLP analyses. Among the rhizospheric isolates of B. cepacia, we found only B. cepacia genomovar I, B. cepacia genomovar III, Burkholderia vietnamiensis (genomovar V) and B. ambifaria. B. cepacia genomovars I and III and B. ambifaria were recovered from all three fields, whereas B. vietnamiensis was detected only in the population isolated from the field located in central Italy. Among strains isolated from northern and southern Italy, the most abundant genomovars were B. ambifaria and B. cepacia genomovar III respectively; in contrast, the population isolated in central Italy showed an even distribution of strains among genomovars. These results indicate that it is not possible to differentiate clinical and environmental strains, or pathogenic and non-pathogenic strains, of the B. cepacia complex simply on the basis of genomovar status, and that the environment may serve as a reservoir for B. cepacia genomovar III infections in vulnerable humans.

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Section: Discussionmentioning

confidence: 99%

Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy

Fiore¹,

Laevens²,

Bevivino³

et al. 2001

Environmental Microbiology

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“…Certain species contribute to soil fertility and crop yield (Leung et al 1994;Borneman et al 1996). Beneficial microorganisms include mycorrhizal fungi, bacteria with biopesticide activity (such as Pseudomonas spp.)…”

Section: Introductionmentioning

confidence: 98%

EvaluatingPseudomonas aeruginosaas plant growth-promoting rhizobacteria in West Africa

Adesemoye

Ugoji

2009

Archives Of Phytopathology And Plant Protection

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Some parameters of growth were examined in three test crops as indices of plant growth-promoting rhizobacteria (PGPR) ability of Pseudomonas aeruginosa. Crops include Abelmoschus esculentus L. (okra), Lycopersicon esculentum L. (tomato) and Amaranthus sp. (African spinach). This study aimed to examine the effectiveness of PGPR in West Africa and determine whether the inoculation method has an impact on PGPR's effectiveness. Bacterium was isolated from topsoil in the Botanical Garden, University of Lagos, Nigeria. Inoculation with bacteria was done by soaking seeds in 10 6 cfu/ml of bacterial suspension and coating was done using 10% starch (w/v) as seed adhesive with 10 6 cfu/ml of bacterial suspension. The third treatment involved soaking seeds in distilled water and later applying NPK 15:15:15 fertilizer. Control seeds were soaked in distilled water. Two methods of bacterial inoculation (soaking and coating) produced statistically similar results to plants grown with fertilizer but performed better than the control, suggesting a high potential of P. aeruginosa as PGPR.

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“…There is thus a broad scope for the introduction of GMMs to contaminated soils but the current knowledge about survival of the released GMMs in such ecosystems and their effect on indigenous microflora is limited [15][16].…”

Section: Introductionmentioning

confidence: 99%

Indigenous microflora and bean responses to introduction of genetically modifiedpseudomonas fluorescensstrains into soil contaminated with copper

Kozdrój

Piotrowska‐Seget

1995

Journal of Environmental Science and Health . Part A: Environme

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Microbial diversity in soil: effect of releasing genetically engineered micro‐organisms

Cited by 24 publications

References 109 publications

Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy

Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy

EvaluatingPseudomonas aeruginosaas plant growth-promoting rhizobacteria in West Africa

Indigenous microflora and bean responses to introduction of genetically modifiedpseudomonas fluorescensstrains into soil contaminated with copper

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