Chemical surface management for micro PCR in silicon chip thermocyclers

Felbel, Jana; Bieber, Ivonne; Koehler, J. Michael

doi:10.1117/12.468657

Cited by 8 publications

(2 citation statements)

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“…Contact angle measurements provide information about the current hydrophobic/hydrophilic state of a surface which seems to be related to the PCR friendliness of a surface [9,10]. For measurement of the water contact angles of the different surfaces the 'Krüss-drop shape analysis system-DSA 10 Mk2' was used.…”

Section: Contact Angle Measurementsmentioning

confidence: 99%

“…Most PCR microsystems have been fabricated in silicon, glass or polymer. Research on silicon/glass PCR microsystems [4,5,[7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] have shown serious interactions between the silicon/glass surface and the PCR ingredients sometimes leading to strong inhibition of the PCR performance. During the last decade, polymer microsystems for PCR have entered this research area [23][24][25][26] and has also shown varying influence on the PCR performance.…”

Section: Introductionmentioning

confidence: 99%

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PCR biocompatibility of lab-on-a-chip and MEMS materials

Christensen

Pedersen

Grøndahl

et al. 2007

J. Micromech. Microeng.

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DNA amplification using the polymerase chain reaction (PCR) is an important tool in biotechnology, pathogen surveillance in food, medical and forensic science etc. The PCR technique is now an important part of the research in and development of miniaturized biochemical analysis systems. However, reduced or no DNA amplification at all is an important challenge for microfabricated PCR devices due to a negative interaction between PCR chemicals and the surrounding environment, i.e. the materials encapsulating the PCR mix. Materials of special interest regarding PCR compatibility are silicon, glass and polymers, which are important in the fabrication of microelectromechanical systems (MEMS), micro total analysis systems (µTAS) and lab-on-a-chip (LOC) systems. The PCR inhibition effect is a particularly important phenomenon in microsystems due to an increased surface-to-volume ratio which enhances the possibility of interaction between the surfaces and ingredients in the PCR mixture. By proper surface treatment the PCR reaction can be facilitated and in this paper we present a systematic and quantitative study of the impact on the PCR compatibility of a chemical and a biological surface treatment. The chemical treatments are based on the silanizing agent dichlordimethylsilane [(CH 3 ) 2 SiCl 2 ]], while the biological treatment is based on the protein bovine serum albumin (BSA). We present a simple model system for the investigation of the PCR compatibility of three widely used materials in microfabrication, namely silicon, glass and SU-8. The impact on PCR performance, measured by means of PCR efficiency, of untreated as well as chemically and biologically treated materials is studied. We show a convenient method of assessing the PCR compatibility of silicon, glass and SU-8 with a degree of information not presented before.

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Section: Contact Angle Measurementsmentioning

confidence: 99%